Skeletal muscle is anabolically unresponsive to an amino acid infusion in pediatric burn patients 6 months postinjury

Demidmaa Tuvdendorj, David L. Chinkes, Xiao Jun Zhang, Melinda Sheffield-Moore, David Herndon

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective: To evaluate leg muscle, whole-body muscle, and whole-body nonmuscle protein response to anabolic signaling of amino acids in pediatric burn patients at 6 months after injury. Background: Burn injury is associated with a catabolic state persisting years after the injury. The tissue response to nutritional signaling (eg, amino acids) plays a critical role in tissue protein net balance via coordination of protein synthesis and breakdown mechanisms. Methods: A total of 10 patients (7.4 ± 3.8 years; 27.4 ± 14.7 kg) and 5 healthy young males (22 ± 3 years; 76 ± 15 kg) underwent an 8-hour stable isotope infusion study. During the last 3 hours, an amino acid solution (10% Travasol, Clintec Nutrition, Deerfield, IL) was infused. Femoral arterial and venous blood samples and muscle biopsy samples were collected throughout the study. A P value of less than 0.05 was considered statistically different. Results:: During amino acid infusion, leg muscle protein synthesis rate significantly increased (P < 0.05) in both groups, however, in the burn group, protein breakdown also increased, although nonsignificantly. As a result, protein net balance remained negative. In the control group, breakdown nonsignificantly decreased resulting in a significant increase (P < 0.05) in muscle protein net balance. Whole-body protein breakdown was significantly higher in the burn patients. Conclusion:: In pediatric burn patients at 6 months postinjury, leg muscle protein net deposition is unresponsive to amino acid infusion; and whole-body protein breakdown is significantly higher than in the control group.

Original languageEnglish (US)
Pages (from-to)592-597
Number of pages6
JournalAnnals of Surgery
Volume253
Issue number3
DOIs
StatePublished - Mar 2011

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Skeletal Muscle
Pediatrics
Amino Acids
Muscle Proteins
Proteins
Leg
Muscles
Wounds and Injuries
Control Groups
Thigh
Isotopes
Biopsy

ASJC Scopus subject areas

  • Surgery

Cite this

Skeletal muscle is anabolically unresponsive to an amino acid infusion in pediatric burn patients 6 months postinjury. / Tuvdendorj, Demidmaa; Chinkes, David L.; Zhang, Xiao Jun; Sheffield-Moore, Melinda; Herndon, David.

In: Annals of Surgery, Vol. 253, No. 3, 03.2011, p. 592-597.

Research output: Contribution to journalArticle

Tuvdendorj, Demidmaa ; Chinkes, David L. ; Zhang, Xiao Jun ; Sheffield-Moore, Melinda ; Herndon, David. / Skeletal muscle is anabolically unresponsive to an amino acid infusion in pediatric burn patients 6 months postinjury. In: Annals of Surgery. 2011 ; Vol. 253, No. 3. pp. 592-597.
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abstract = "Objective: To evaluate leg muscle, whole-body muscle, and whole-body nonmuscle protein response to anabolic signaling of amino acids in pediatric burn patients at 6 months after injury. Background: Burn injury is associated with a catabolic state persisting years after the injury. The tissue response to nutritional signaling (eg, amino acids) plays a critical role in tissue protein net balance via coordination of protein synthesis and breakdown mechanisms. Methods: A total of 10 patients (7.4 ± 3.8 years; 27.4 ± 14.7 kg) and 5 healthy young males (22 ± 3 years; 76 ± 15 kg) underwent an 8-hour stable isotope infusion study. During the last 3 hours, an amino acid solution (10{\%} Travasol, Clintec Nutrition, Deerfield, IL) was infused. Femoral arterial and venous blood samples and muscle biopsy samples were collected throughout the study. A P value of less than 0.05 was considered statistically different. Results:: During amino acid infusion, leg muscle protein synthesis rate significantly increased (P < 0.05) in both groups, however, in the burn group, protein breakdown also increased, although nonsignificantly. As a result, protein net balance remained negative. In the control group, breakdown nonsignificantly decreased resulting in a significant increase (P < 0.05) in muscle protein net balance. Whole-body protein breakdown was significantly higher in the burn patients. Conclusion:: In pediatric burn patients at 6 months postinjury, leg muscle protein net deposition is unresponsive to amino acid infusion; and whole-body protein breakdown is significantly higher than in the control group.",
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