Skeletal muscle loss is associated with TNF mediated insufficient skeletal myogenic activation after burn

Juquan Song, Melody R. Saeman, Jana De Libero, Steven Wolf

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Muscle loss accompanies severe burn; in this hyper-catabolic state, muscle undergoes atrophy through protein degradation and disuse. Muscle volume is related to the relative rates of cellular degradation and myogenesis. We hypothesize that muscle atrophy after injury is in part because of insufficient myogenesis associated with the hyperinflammatory response. The aim of this study was to investigate the role of skeletalmyogenesis and muscle cell homeostasis in response to severe burn. Twenty-eight male C57BL6 mice received 25% TBSA scald. Gluteus muscle from these animals was analyzed at days 1, 3, 7, and 14 after injury. Six additional animals without burn served as controls.We showed muscle wet weight and protein content decreased at days 3 and 7 after burn, with elevated tumor necrosis factor (TNF) mRNA expression (P<0.05). Increased cell death was observed through TUNEL staining, and cleaved caspase-3 levels reached a peak in muscle lysate at day 3 (P<0.05). The cell proliferation marker proliferating cell nuclear antigen (PCNA) significantly increased after burn, associated with increased gene and protein expression of myogenesis markers Pax7 and myogenin. Desmin mRNA expression and the ratio of desmin to PCNA protein expression, however, significantly decreased at day 7 (P<0.05). In vitro, the ratio of desmin to PCNA protein expression significantly decreased in C2C12 murine myoblasts after TNF-a stimulation for 24 h. We showed that severe burn induces both increased cell death and proliferation. Myogenesis, however, does not counterbalance increased cell death after burn. Data suggest insufficient myogenesis might be associated with pro-inflammatory mediator TNF activity.

Original languageEnglish (US)
Pages (from-to)479-486
Number of pages8
JournalShock
Volume44
Issue number5
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Fingerprint

Muscle Development
Skeletal Muscle
Tumor Necrosis Factor-alpha
Desmin
Proliferating Cell Nuclear Antigen
Muscles
Cell Death
Muscular Atrophy
Nuclear Proteins
Cell Proliferation
Myogenin
Messenger RNA
Myoblasts
In Situ Nick-End Labeling
Wounds and Injuries
Caspase 3
Muscle Cells
Proteolysis
Proteins
Homeostasis

Keywords

  • Burn
  • Gluteus muscles
  • Mouse
  • Myogenic regulatory factors (MRFs)
  • satellite cell

ASJC Scopus subject areas

  • Emergency Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Skeletal muscle loss is associated with TNF mediated insufficient skeletal myogenic activation after burn. / Song, Juquan; Saeman, Melody R.; De Libero, Jana; Wolf, Steven.

In: Shock, Vol. 44, No. 5, 01.01.2015, p. 479-486.

Research output: Contribution to journalArticle

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