Skeletal muscle protein anabolic response to increased energy and insulin is preserved in poorly controlled type 2 diabetes

Jill A. Bell, Elena Volpi, Satoshi Fujita, Jerson G. Cadenas, Melinda Sheffield-Moore, Blake Rasmussen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Type 2 diabetes (T2DM) subjects failing diet treatment are characterized by hyperinsulinemia and insulin resistance leading to fasting and postprandial hyperglycemia and hyperlipidemia. Energy is essential for allowing the process of protein synthesis to proceed. Additionally, insulin can stimulate protein synthesis in human muscle. The aims of this study were to determine if poorly controlled T2DM affects postabsorptive muscle protein anabolism, and if the muscle anabolic response to hyperinsulinemia with high energy availability is maintained. Control (n = 6) and T2DM subjects (n = 6) were studied in the postabsorptive state and during an isoenergetic high nutritional energy clamp (relative to postabsorptive state). Muscle protein synthesis and breakdown (nmol·min-1·100 g leg muscle-1) were assessed using stable isotope methodology, femoral arterio-venous sampling, muscle biopsies, and a three-pool model to calculate protein turnover. Postabsorptive phenylalanine net balance and whole body rate of appearance (Ra) were not different between groups; however, basal muscle protein breakdown was higher in T2DM (94 ± 9) than in controls (58 ± 12) (P < 0.05) and muscle protein synthesis tended (P = 0.07) to be elevated in T2DM (66 ± 14) compared with controls (39 ± 6). During the clamp, net balance increased, whole body Ra and muscle protein breakdown decreased (P < 0.05), and muscle protein synthesis tended to decrease (P = 0.08) to a similar extent in both groups. We conclude that postabsorptive muscle protein turnover is elevated in poorly controlled T2DM, however, there is no excessive loss of muscle protein because net balance is not different from controls. Moreover, the anabolic response to increased insulin and energy availability is maintained in T2DM.

Original languageEnglish (US)
Pages (from-to)1249-1255
Number of pages7
JournalJournal of Nutrition
Volume136
Issue number5
StatePublished - May 2006
Externally publishedYes

Fingerprint

Muscle Proteins
muscle protein
noninsulin-dependent diabetes mellitus
Type 2 Diabetes Mellitus
skeletal muscle
Skeletal Muscle
insulin
Insulin
protein synthesis
energy
Muscles
muscles
hyperinsulinemia
Hyperinsulinism
protein metabolism
Proteins
hyperlipidemia
hyperglycemia
thighs
Thigh

Keywords

  • Muscle protein synthesis
  • Protein metabolism
  • Protein turnover
  • Type 2 diabetes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Skeletal muscle protein anabolic response to increased energy and insulin is preserved in poorly controlled type 2 diabetes. / Bell, Jill A.; Volpi, Elena; Fujita, Satoshi; Cadenas, Jerson G.; Sheffield-Moore, Melinda; Rasmussen, Blake.

In: Journal of Nutrition, Vol. 136, No. 5, 05.2006, p. 1249-1255.

Research output: Contribution to journalArticle

Bell, Jill A. ; Volpi, Elena ; Fujita, Satoshi ; Cadenas, Jerson G. ; Sheffield-Moore, Melinda ; Rasmussen, Blake. / Skeletal muscle protein anabolic response to increased energy and insulin is preserved in poorly controlled type 2 diabetes. In: Journal of Nutrition. 2006 ; Vol. 136, No. 5. pp. 1249-1255.
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