SLIP1, a factor required for activation of histone mRNA translation by the stem-loop binding protein

Nihal G. Cakmakci, Rachel S. Lerner, Eric Wagner, Lianxing Zheng, William F. Marzluff

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Replication-dependent histone mRNAs are the only eukaryotic cellular mRNAs that are not polyadenylated, ending instead in a conserved stem-loop. The 3′ end of histone mRNA is required for histone mRNA translation, as is the stem-loop binding protein (SLBP), which binds the 3′ end of histone mRNA. We have identified five conserved residues in a 15-amino-acid region in the amino-terminal portion of SLBP, each of which is required for translation. Using a yeast two-hybrid screen, we identified a novel protein, SLBP-interacting protein 1 (SLIP1), that specifically interacts with this region. Mutations in any of the residues required for translation reduces SLIP1 binding to SLBP. The expression of SLIP1 in Xenopus oocytes together with human SLBP stimulates translation of a reporter mRNA ending in the stem-loop but not a reporter with a poly (A) tail. The expression of SLIP1 in HeLa cells also stimulates the expression of a green fluorescent protein reporter mRNA ending in a stem-loop. RNA interference-mediated downregulation of endogenous SLIP1 reduces the rate of translation of endogenous histone mRNA and also reduces cell viability. SLIP1 may function by bridging the 3′ end of the histone mRNA with the 5′ end of the mRNA, similar to the mechanism of translation of polyadenylated mRNAs.

Original languageEnglish (US)
Pages (from-to)1182-1194
Number of pages13
JournalMolecular and Cellular Biology
Volume28
Issue number3
DOIs
StatePublished - Feb 2008
Externally publishedYes

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Protein Biosynthesis
Histones
Carrier Proteins
Messenger RNA
Proteins
Xenopus
RNA Interference
Green Fluorescent Proteins
HeLa Cells
Protein Binding
Oocytes
Cell Survival
Down-Regulation
Yeasts
Amino Acids
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

SLIP1, a factor required for activation of histone mRNA translation by the stem-loop binding protein. / Cakmakci, Nihal G.; Lerner, Rachel S.; Wagner, Eric; Zheng, Lianxing; Marzluff, William F.

In: Molecular and Cellular Biology, Vol. 28, No. 3, 02.2008, p. 1182-1194.

Research output: Contribution to journalArticle

Cakmakci, Nihal G. ; Lerner, Rachel S. ; Wagner, Eric ; Zheng, Lianxing ; Marzluff, William F. / SLIP1, a factor required for activation of histone mRNA translation by the stem-loop binding protein. In: Molecular and Cellular Biology. 2008 ; Vol. 28, No. 3. pp. 1182-1194.
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