Slow recovery of body fat lost during adenovirus-induced hyperleptinemia

Moritake Higa, Tetsuya Kakuma, Wentong Pan, Zhuo Wei Wang, Evelyn Babcock, Kay McCorkle, Young Lee, Roger Unger

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In normal rats, adenovirus-induced hyperleptinemia causes disappearance of visible body fat, down-regulation of lipogenic enzymes, and upregulation of oxidative enzymes and thermogenic proteins. In addition, preadipocyte markers replace mature adipocyte markers, suggesting dedifferentiation. In weight loss induced by caloric restriction, by contrast, the lipogenic machinery is essentially intact. To determine if the radical changes induced by leptin would slow the reappearance of body fat, we compared normal lean rats made hyperleptinemic by infusing an adenovirusleptin construct with diet-matched littermates. Initially, in plasma leptin the hyperleptinemic rats averaged @O50x the controls and, although it declined progressively, it was still slightly elevated at 150 days (P 〈 0.05). In the hyperleptinemics, body fat mass, quantified by magnetic resonance spectroscopy, remained below the pretreatment value for 60 days, while in diet-matched controls it exceeded the pretreatment value. Epididymal fat pad weight in hyperleptinemics was still 28% below paired controls at 150 days posttreatment. Histologic examination revealed adipocytes of hyperleptinemic animals to be smaller 60 days after treatment. At 60 days, adipose tissue UCP-2 gene expression in hyperleptinemics was still above controls, but expression of other lipogenic and oxidative enzymes had returned to baseline expression levels. We conclude that in normal rats recovery of body fat following adenovirus-induced hyperleptinemia is much slower than after caloric restriction, possibly because of persistent upregulation of adipocyte UCP-2.

Original languageEnglish (US)
Pages (from-to)786-791
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume279
Issue number3
DOIs
StatePublished - Dec 29 2000
Externally publishedYes

Fingerprint

Adenoviridae
Adipose Tissue
Fats
Rats
Recovery
Adipocytes
Caloric Restriction
Nutrition
Leptin
Enzymes
Magnetic resonance spectroscopy
Up-Regulation
Diet
Gene expression
Machinery
Animals
Weight Loss
Tissue
Magnetic Resonance Spectroscopy
Plasmas

Keywords

  • Caloric restriction
  • Hyperleptinemia
  • UCP-2

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Slow recovery of body fat lost during adenovirus-induced hyperleptinemia. / Higa, Moritake; Kakuma, Tetsuya; Pan, Wentong; Wang, Zhuo Wei; Babcock, Evelyn; McCorkle, Kay; Lee, Young; Unger, Roger.

In: Biochemical and Biophysical Research Communications, Vol. 279, No. 3, 29.12.2000, p. 786-791.

Research output: Contribution to journalArticle

Higa, M, Kakuma, T, Pan, W, Wang, ZW, Babcock, E, McCorkle, K, Lee, Y & Unger, R 2000, 'Slow recovery of body fat lost during adenovirus-induced hyperleptinemia', Biochemical and Biophysical Research Communications, vol. 279, no. 3, pp. 786-791. https://doi.org/10.1006/bbrc.2000.4025
Higa, Moritake ; Kakuma, Tetsuya ; Pan, Wentong ; Wang, Zhuo Wei ; Babcock, Evelyn ; McCorkle, Kay ; Lee, Young ; Unger, Roger. / Slow recovery of body fat lost during adenovirus-induced hyperleptinemia. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 279, No. 3. pp. 786-791.
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