SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation

Xin Jie Zhuang, Yu Qiang Shi, Bo Xu, Lei Chen, Wen Hao Tang, Jin Huang, Ying Lian, Ping Liu, Jie Qiao

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Gametogenesis is a complex biological process of producing cells for sexual reproduction. Xlr super family members containing a conserved COR1 domain play essential roles in gametogenesis. In the present study, we identified that Slx2, a novel member of Xlr super family, is specifically expressed in the meiotic oocytes, which is demonstrated by western blotting and immunohistochemistry studies. In the first meiotic prophase, SLX2 is unevenly distributed in the nuclei of oocytes, during which phase SLX2 is partly co-localized with SYCP3 in synaptonemal complex and ã H2AX in the nucleus of oocytes. Interestingly, the localization of SLX2 was found to be switched into the cytoplasm of oocytes after prometaphase I during oocyte maturation. Furthermore, yeast two-hybrid and coimmunoprecipitation studies demonstrated that SLX2 interacts with BLOS2, which is a novel centrosome-associated protein, and co-localized with γ-Tubulin, which is a protein marker of chromosome segregation in meiosis. These results indicated that SLX2 might get involved in chromosomes segregation during meiosis by interaction with BLOS2. In conclusion, SLX2 might be a novel gametogenesis-related protein that could play multiple roles in regulation of meiotic processes including synaptonemal complex assembly and chromosome segregation.

Original languageEnglish (US)
Pages (from-to)2231-2237
Number of pages7
JournalCell Cycle
Volume13
Issue number14
DOIs
StatePublished - Jul 15 2014
Externally publishedYes

Fingerprint

Oocytes
Gametogenesis
Chromosome Segregation
Synaptonemal Complex
Meiosis
Prometaphase
Biological Phenomena
Prophase
Centrosome
Proteins
Tubulin
Reproduction
Cytoplasm
Yeasts
Western Blotting
Immunohistochemistry

Keywords

  • BLOS2
  • Meiosis
  • Oocyte
  • SLX2
  • Spindle

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology
  • Medicine(all)

Cite this

Zhuang, X. J., Shi, Y. Q., Xu, B., Chen, L., Tang, W. H., Huang, J., ... Qiao, J. (2014). SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation. Cell Cycle, 13(14), 2231-2237. https://doi.org/10.4161/cc.29265

SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation. / Zhuang, Xin Jie; Shi, Yu Qiang; Xu, Bo; Chen, Lei; Tang, Wen Hao; Huang, Jin; Lian, Ying; Liu, Ping; Qiao, Jie.

In: Cell Cycle, Vol. 13, No. 14, 15.07.2014, p. 2231-2237.

Research output: Contribution to journalArticle

Zhuang, XJ, Shi, YQ, Xu, B, Chen, L, Tang, WH, Huang, J, Lian, Y, Liu, P & Qiao, J 2014, 'SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation', Cell Cycle, vol. 13, no. 14, pp. 2231-2237. https://doi.org/10.4161/cc.29265
Zhuang XJ, Shi YQ, Xu B, Chen L, Tang WH, Huang J et al. SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation. Cell Cycle. 2014 Jul 15;13(14):2231-2237. https://doi.org/10.4161/cc.29265
Zhuang, Xin Jie ; Shi, Yu Qiang ; Xu, Bo ; Chen, Lei ; Tang, Wen Hao ; Huang, Jin ; Lian, Ying ; Liu, Ping ; Qiao, Jie. / SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation. In: Cell Cycle. 2014 ; Vol. 13, No. 14. pp. 2231-2237.
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