SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation

Xin Jie Zhuang, Yu Qiang Shi, Bo Xu, Lei Chen, Wen Hao Tang, Jin Huang, Ying Lian, Ping Liu, Jie Qiao

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Gametogenesis is a complex biological process of producing cells for sexual reproduction. Xlr super family members containing a conserved COR1 domain play essential roles in gametogenesis. In the present study, we identified that Slx2, a novel member of Xlr super family, is specifically expressed in the meiotic oocytes, which is demonstrated by western blotting and immunohistochemistry studies. In the first meiotic prophase, SLX2 is unevenly distributed in the nuclei of oocytes, during which phase SLX2 is partly co-localized with SYCP3 in synaptonemal complex and ã H2AX in the nucleus of oocytes. Interestingly, the localization of SLX2 was found to be switched into the cytoplasm of oocytes after prometaphase I during oocyte maturation. Furthermore, yeast two-hybrid and coimmunoprecipitation studies demonstrated that SLX2 interacts with BLOS2, which is a novel centrosome-associated protein, and co-localized with γ-Tubulin, which is a protein marker of chromosome segregation in meiosis. These results indicated that SLX2 might get involved in chromosomes segregation during meiosis by interaction with BLOS2. In conclusion, SLX2 might be a novel gametogenesis-related protein that could play multiple roles in regulation of meiotic processes including synaptonemal complex assembly and chromosome segregation.

Original languageEnglish (US)
Pages (from-to)2230-2236
Number of pages7
JournalCell Cycle
Volume13
Issue number14
DOIs
StatePublished - Jul 15 2014

Keywords

  • BLOS2
  • Meiosis
  • Oocyte
  • SLX2
  • Spindle

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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    Zhuang, X. J., Shi, Y. Q., Xu, B., Chen, L., Tang, W. H., Huang, J., Lian, Y., Liu, P., & Qiao, J. (2014). SLX2 interacting with BLOS2 is differentially expressed during mouse oocyte meiotic maturation. Cell Cycle, 13(14), 2230-2236. https://doi.org/10.4161/cc.29265