Small Molecule Compounds That Inhibit Antioxidant Response Gene Expression in an Inducer-Dependent Manner

Megan R. Edwards, Gai Liu, Sampriti De, Julien Sourimant, Colette Pietzsch, Britney Johnson, Gaya K. Amarasinghe, Daisy W. Leung, Alexander Bukreyev, Richard K. Plemper, Zachary Aron, Terry L. Bowlin, Donald T. Moir, Christopher F. Basler

    Research output: Contribution to journalArticle

    Abstract

    Marburg virus (MARV) causes severe disease in humans and is known to activate nuclear factor erythroid 2-related factor 2 (Nrf2), the major transcription factor of the antioxidant response. Canonical activation of Nrf2 involves oxidative or electrophilic stress that prevents Kelch-like ECH-associated protein 1 (Keap1) targeted degradation of Nrf2, leading to Nrf2 stabilization and activation of the antioxidant response. MARV activation of Nrf2 is noncanonical with the MARV VP24 protein (mVP24) interacting with Keap1, freeing Nrf2 from degradation. A high-throughput screening (HTS) assay was developed to identify inhibitors of mVP24-induced Nrf2 activity and used to screen more than 55,000 compounds. Hit compounds were further screened against secondary HTS assays for the inhibition of antioxidant activity induced by additional canonical and noncanonical mechanisms. This pipeline identified 14 compounds that suppress the response, dependent on the inducer, with 50% inhibitory concentrations below 5 μM and selectivity index values greater than 10. Notably, several of the identified compounds specifically inhibit mVP24-induced Nrf2 activity.

    Original languageEnglish (US)
    Pages (from-to)489-502
    Number of pages14
    JournalACS Infectious Diseases
    Volume6
    Issue number3
    DOIs
    StatePublished - Mar 13 2020

    Keywords

    • antioxidant response
    • high-throughput screen
    • Marburg virus
    • Nrf2

    ASJC Scopus subject areas

    • Infectious Diseases

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  • Cite this

    Edwards, M. R., Liu, G., De, S., Sourimant, J., Pietzsch, C., Johnson, B., Amarasinghe, G. K., Leung, D. W., Bukreyev, A., Plemper, R. K., Aron, Z., Bowlin, T. L., Moir, D. T., & Basler, C. F. (2020). Small Molecule Compounds That Inhibit Antioxidant Response Gene Expression in an Inducer-Dependent Manner. ACS Infectious Diseases, 6(3), 489-502. https://doi.org/10.1021/acsinfecdis.9b00416