Smooth muscle uses another promoter to express primarily a form of human CaV1.2 L-type calcium channel different from the principal heart form

Nehad Saada, Bosong Dai, Clement Echetebu, Sushil K. Sarna, Philip Palade

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Several different first exons and amino termini have been reported for the cardiac Ca channel known as α1C or CaV1.2. The aim of this study was to investigate whether the expression of this channel is regulated by different promoters in smooth muscle cells and in heart in humans. Ribonuclease protection assay (RPA) indicates that the longer first exon 1a is found in certain human smooth muscle-containing tissues, notably bladder and fetal aorta, but that it is not expressed to any significant degree in lung or intestine. On the other hand, all four smooth muscle-containing tissues examined strongly express transcripts containing exon 1b, first reported cloned from human fibroblast cells [1]. In addition, primary cultures of human colonic myocytes and coronary artery smooth muscle cells express predominantly transcripts containing exon 1b. The promoter immediately upstream of exon 1b was cloned, and it displays functional promoter activity when luciferase-expressing constructs were transfected into three different cultured smooth muscle cells: primary human coronary artery smooth muscles cells, primary human colonocytes, and the fetal rat aorta-derived A7r5 cell line. These results indicate that expression in smooth muscle is primarily driven by a promoter different from that which drives expression in cardiac myocytes.

Original languageEnglish (US)
Pages (from-to)23-28
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume302
Issue number1
DOIs
StatePublished - Feb 28 2003

Keywords

  • Human heart
  • Human smooth muscle
  • L-type calcium channel
  • Promoter

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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