Sodium hypochlorite decontamination of split-thickness cadaveric skin infected with bacteria and yeast with subsequent isolation and growth of basal cells to confluency in tissue culture

R. C. Fader, A. Maurer, M. D. Stein, S. Abston, David Herndon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The ability of sodium hypochlorite to decontaminate skin while leaving sufficient epidermal cell viability for growth in tissue culture was investigated with an in vitro system. Split-thickness cadaveric skin was infected with Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans and subsequently treated with various concentrations of sodium hypochlorite for various time intervals. Exposure to a 0.5% solution of sodium hypochlorite for 6 min effectively decontaminated the skin while leaving 66% of the basal cells viable. The basal cells were subsequently grown to confluency in tissue culture. This study demonstrates that microbial colonization of skin can be eliminated by exposure to dilute hypochlorite. This procedure, while decontaminating the skin, leaves sufficient viability of epidermal cells for subsequent growth and expansion in tissue culture, elements essential for grafting over wounds.

Original languageEnglish (US)
Pages (from-to)181-185
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume24
Issue number2
StatePublished - 1983
Externally publishedYes

Fingerprint

Sodium Hypochlorite
Decontamination
Yeasts
Bacteria
Skin
Growth
Cell Survival
Tissue Expansion
Hypochlorous Acid
Candida albicans
Pseudomonas aeruginosa
Staphylococcus aureus
Wounds and Injuries

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Sodium hypochlorite decontamination of split-thickness cadaveric skin infected with bacteria and yeast with subsequent isolation and growth of basal cells to confluency in tissue culture. / Fader, R. C.; Maurer, A.; Stein, M. D.; Abston, S.; Herndon, David.

In: Antimicrobial Agents and Chemotherapy, Vol. 24, No. 2, 1983, p. 181-185.

Research output: Contribution to journalArticle

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