Abstract
Glycerol alone or in combination with other additives is one of the most widely used and successful cryoprotectants for human sperm. The glycerol method requires rigorous post thaw sample washing for use in ART and this may lead to low sperm yield from oligospermic samples. In this study the feasibility of the use of sucrose in sperm cryopreservation was explored. Sucrose as cryoprotectant was combined with direct plunging of sample into liquid nitrogen (vitrification) as a freezing method. Sucrose treated sperm from normozoospermic and severly oligozoospermic samples underwent rapid freeze and thaw. Motility and viability were evaluated before freezing (after sucrose equilibration) as well as post freezing (after thaw). The 100 mM concentration of sucrose showed better cryoprotectant features compared to that of higher concentrations (200-1000 mM). Sucrose (100 mM)treated sperm maintained low but acceptable motility (30%) and satisfactory viability (60%) after freezing and thawing. The cryoprotectant capacity of sucrose for normozoospermic and oligozoospermic samples were identical. The sucrose method utilizes rapid freezing of a micro volume of sample and thus quickly freezes, thaws, and maximizes recovery of the sperm from the sample.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 99-103 |
| Number of pages | 5 |
| Journal | Systems Biology in Reproductive Medicine |
| Volume | 53 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2007 |
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Keywords
- cryoprotectant
- oligozoospermic sample
- sperm freezing
- sucrose
ASJC Scopus subject areas
- Reproductive Medicine
- Urology
Cite this
Sole use of sucrose in human sperm cryopreservation. / Hossain, Amjad; Osuamkpe, C. O.
In: Systems Biology in Reproductive Medicine, Vol. 53, No. 2, 2007, p. 99-103.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sole use of sucrose in human sperm cryopreservation
AU - Hossain, Amjad
AU - Osuamkpe, C. O.
PY - 2007
Y1 - 2007
N2 - Glycerol alone or in combination with other additives is one of the most widely used and successful cryoprotectants for human sperm. The glycerol method requires rigorous post thaw sample washing for use in ART and this may lead to low sperm yield from oligospermic samples. In this study the feasibility of the use of sucrose in sperm cryopreservation was explored. Sucrose as cryoprotectant was combined with direct plunging of sample into liquid nitrogen (vitrification) as a freezing method. Sucrose treated sperm from normozoospermic and severly oligozoospermic samples underwent rapid freeze and thaw. Motility and viability were evaluated before freezing (after sucrose equilibration) as well as post freezing (after thaw). The 100 mM concentration of sucrose showed better cryoprotectant features compared to that of higher concentrations (200-1000 mM). Sucrose (100 mM)treated sperm maintained low but acceptable motility (30%) and satisfactory viability (60%) after freezing and thawing. The cryoprotectant capacity of sucrose for normozoospermic and oligozoospermic samples were identical. The sucrose method utilizes rapid freezing of a micro volume of sample and thus quickly freezes, thaws, and maximizes recovery of the sperm from the sample.
AB - Glycerol alone or in combination with other additives is one of the most widely used and successful cryoprotectants for human sperm. The glycerol method requires rigorous post thaw sample washing for use in ART and this may lead to low sperm yield from oligospermic samples. In this study the feasibility of the use of sucrose in sperm cryopreservation was explored. Sucrose as cryoprotectant was combined with direct plunging of sample into liquid nitrogen (vitrification) as a freezing method. Sucrose treated sperm from normozoospermic and severly oligozoospermic samples underwent rapid freeze and thaw. Motility and viability were evaluated before freezing (after sucrose equilibration) as well as post freezing (after thaw). The 100 mM concentration of sucrose showed better cryoprotectant features compared to that of higher concentrations (200-1000 mM). Sucrose (100 mM)treated sperm maintained low but acceptable motility (30%) and satisfactory viability (60%) after freezing and thawing. The cryoprotectant capacity of sucrose for normozoospermic and oligozoospermic samples were identical. The sucrose method utilizes rapid freezing of a micro volume of sample and thus quickly freezes, thaws, and maximizes recovery of the sperm from the sample.
KW - cryoprotectant
KW - oligozoospermic sample
KW - sperm freezing
KW - sucrose
UR - http://www.scopus.com/inward/record.url?scp=79961188673&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79961188673&partnerID=8YFLogxK
U2 - 10.1080/01485010701225675
DO - 10.1080/01485010701225675
M3 - Article
C2 - 17453690
AN - SCOPUS:34247341772
VL - 53
SP - 99
EP - 103
JO - Systems Biology in Reproductive Medicine
JF - Systems Biology in Reproductive Medicine
SN - 1939-6368
IS - 2
ER -