Solid-phase immunosorbent technique for rapid detection of Rift Valley fever virus immunoglobulin M by hemagglutination inhibition

A. K. Soliman, B. A M Botros, J. C. Morrill

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A solid-phase immunosorbent technique (SPIT) was adapted to detect Rift Valley fever (RVF) virus-specific immunoglobulin M (IgM) in serum samples from humans vaccinated with Formalin-inactivated RVF vaccine. Microdilution plates coated with goat anti-human IgM were successively incubated with serum samples from human vaccinees, RVF virus hemagglutinating antigen, and goose erythrocytes. The RVF virus-specific IgM in the serum samples from vaccinees bound to the RVF virus antigen and inhibited hemagglutination of goose erythrocytes. SPIT was compared to the IGM capture enzyme linked immunosorbent assay (ELISA) and the indirect immunofluorescent-antibody (IFA) assay and was found to be sensitive in detecting RVF virus-specific IgM antibody, with high correlations between SPIT and the other two tests (Pearson's correlation coefficient [r] = 0.9 and 0.6, respectively). Results fo SPIT were obtained within 5 h, offering speed over ELISA (8 h). In addition, SPIT does not requrie sophisticated equipment or expensive reagents. Serum theumatoid factor did not produce false-positive reactions in SPIT as in the indirect immunofluorescent-antibody assay and IgM capture ELISA.

Original languageEnglish (US)
Pages (from-to)1913-1915
Number of pages3
JournalJournal of Clinical Microbiology
Volume26
Issue number9
StatePublished - 1988
Externally publishedYes

Fingerprint

Rift Valley fever virus
Immunosorbent Techniques
Hemagglutination
Immunoglobulin M
Geese
Enzyme-Linked Immunosorbent Assay
Serum
Antibodies
Rift Valley Fever
Erythrocytes
False Positive Reactions
Antigens
Goats
Formaldehyde
Vaccines
Equipment and Supplies

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Solid-phase immunosorbent technique for rapid detection of Rift Valley fever virus immunoglobulin M by hemagglutination inhibition. / Soliman, A. K.; Botros, B. A M; Morrill, J. C.

In: Journal of Clinical Microbiology, Vol. 26, No. 9, 1988, p. 1913-1915.

Research output: Contribution to journalArticle

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