Solid phase radioimmunoassay for antibodies to flavivirus structural and nonstructural proteins

D. W. Trent, C. L. Harvey, A. Qureshi, D. LeStourgeon

Research output: Contribution to journalArticle

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Abstract

A micro solid phase radioimmunoassay (SPRIA) is described for quantitation of antibodies to purified flaviviruses as well as to the purified envelope glycoprotein and 80,000 molecular weight viral nonstructural protein. Sera from mice experimentally infected with Saint Louis encephalitis (SLE) virus or from humans after a primary SLE virus infection reacted more specifically with the major viral envelope protein in the SPRIA test than with antigens conventionally used in the complement fixation (CF) and hemagglutination inhibition tests. A high degree of correlation (P <0.05) was observed between SPRIA anti immunoglobulin G binding values with the 80,000 molecular weight nonstructural protein of SLE virus and antibody titers obtained by plaque reduction neutralization and CF with the nonstructural protein. In five of seven human sera in which CF antibody titers to the nonstructural protein were 4 or less, SPRIA testing revealed significant titers of IgG immunoglobulin reactive with this viral protein. The SPRIA test for antibodies reactive with group B togavirus nonstructural protein is as specific and sensitive as the plaque reduction neutralization test for titrating viral antibody in human and animal sera. Antibodies reactive with viral envelope proteins are broadly cross reactive by the SPRIA technique, demonstrating both group and complex reactive antigenic determinants. The SPRIA test, using wells precoated with antigen, can be completed in 1 day, providing a rapid, highly sensitive test which can be adapted to use in testing a large number of sera.

Original languageEnglish (US)
Pages (from-to)1325-1333
Number of pages9
JournalInfection and Immunity
Volume13
Issue number5
StatePublished - 1976

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Flavivirus
Radioimmunoassay
St. Louis Encephalitis Viruses
Antibodies
Proteins
Viral Envelope Proteins
Serum
Viral Nonstructural Proteins
Togaviridae
Immunoglobulin G
Molecular Weight
Viral Antibodies
Hemagglutination Inhibition Tests
Antigens
Neutralization Tests
Viral Proteins
Virus Diseases
Viral Load
Immunoglobulins
Epitopes

ASJC Scopus subject areas

  • Immunology

Cite this

Trent, D. W., Harvey, C. L., Qureshi, A., & LeStourgeon, D. (1976). Solid phase radioimmunoassay for antibodies to flavivirus structural and nonstructural proteins. Infection and Immunity, 13(5), 1325-1333.

Solid phase radioimmunoassay for antibodies to flavivirus structural and nonstructural proteins. / Trent, D. W.; Harvey, C. L.; Qureshi, A.; LeStourgeon, D.

In: Infection and Immunity, Vol. 13, No. 5, 1976, p. 1325-1333.

Research output: Contribution to journalArticle

Trent, DW, Harvey, CL, Qureshi, A & LeStourgeon, D 1976, 'Solid phase radioimmunoassay for antibodies to flavivirus structural and nonstructural proteins', Infection and Immunity, vol. 13, no. 5, pp. 1325-1333.
Trent, D. W. ; Harvey, C. L. ; Qureshi, A. ; LeStourgeon, D. / Solid phase radioimmunoassay for antibodies to flavivirus structural and nonstructural proteins. In: Infection and Immunity. 1976 ; Vol. 13, No. 5. pp. 1325-1333.
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