Soluble glucan reverses burn-induced suppression of interleukin-12 and interferon gamma secretion in thermally injured mice

Aristides Koutrouvelis, Cheng Lin, Edward Sherwood

Research output: Contribution to journalArticle

Abstract

Introduction: Burn injury often induces immunosuppression, which is characterized, in part, by impairment of interleukin-12 (IL-12) and Interferon gamma (IFN-γ) secretion. Soluble β-glucans enhance host resistance to a broad spectrum of microbial pathogens. The present study was designed to determine whether glucan would reverse burn-induced suppression of IL-12 and IFN-γ secretion in vivo. Methods: Balb/C mice (4 mice/group) received 25% full-thickness flame burns under general anesthesia. Sham-burned mice served as controls. All mice were immediately resuscitated with normal saline (4 ml/ mouse IP) or saline containing glucan (40 mg/kg). On days 2 and 5 after burns, mice were challenged with E. coli endotoxin (LPS, 4 mg/kg IP) and serum samples were harvested for analysis of IL-12 and IFNγ levels using ELISA. Results: Priming of sham mice with glucan increased IL-12 secretion by 30-50% compared to saline-treated sham mice on days 2 and 5 after burn injury (see figure). Burn injury suppressed IL-12 secretion by 50-70%, yet glucan therapy restored serum IL-12 levels to those observed in non-burned control mice. Glucan priming enhanced IFNγ secretion in sham mice by 50-90% compared to saline-treated controls 6 hours after LPS challenge. Thermal injury suppressed LPS-induced IFNγ secretion by 50-60%. Treatment of burned mice with glucan restored LPS-induced IFNγ levels to those observed in non-burned controls. Conclusions: Thermal injury results in suppression of both IL-12 and IFNγ secretion. Treatment of thermally injured mice with glucan restores IL-12 and IFNγ levels to those observed in non-burned mice. Glucan may serve as an adjunctive immunomodulator to prevent burn-induced immunosuppression.

Original languageEnglish (US)
JournalCritical Care Medicine
Volume27
Issue number12 SUPPL.
StatePublished - 1999

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Glucans
Interleukin-12
Burns
Interferon-gamma
Wounds and Injuries
Immunosuppression
Hot Temperature
Immunologic Factors
Serum
Endotoxins
General Anesthesia
Therapeutics

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

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Soluble glucan reverses burn-induced suppression of interleukin-12 and interferon gamma secretion in thermally injured mice. / Koutrouvelis, Aristides; Lin, Cheng; Sherwood, Edward.

In: Critical Care Medicine, Vol. 27, No. 12 SUPPL., 1999.

Research output: Contribution to journalArticle

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title = "Soluble glucan reverses burn-induced suppression of interleukin-12 and interferon gamma secretion in thermally injured mice",
abstract = "Introduction: Burn injury often induces immunosuppression, which is characterized, in part, by impairment of interleukin-12 (IL-12) and Interferon gamma (IFN-γ) secretion. Soluble β-glucans enhance host resistance to a broad spectrum of microbial pathogens. The present study was designed to determine whether glucan would reverse burn-induced suppression of IL-12 and IFN-γ secretion in vivo. Methods: Balb/C mice (4 mice/group) received 25{\%} full-thickness flame burns under general anesthesia. Sham-burned mice served as controls. All mice were immediately resuscitated with normal saline (4 ml/ mouse IP) or saline containing glucan (40 mg/kg). On days 2 and 5 after burns, mice were challenged with E. coli endotoxin (LPS, 4 mg/kg IP) and serum samples were harvested for analysis of IL-12 and IFNγ levels using ELISA. Results: Priming of sham mice with glucan increased IL-12 secretion by 30-50{\%} compared to saline-treated sham mice on days 2 and 5 after burn injury (see figure). Burn injury suppressed IL-12 secretion by 50-70{\%}, yet glucan therapy restored serum IL-12 levels to those observed in non-burned control mice. Glucan priming enhanced IFNγ secretion in sham mice by 50-90{\%} compared to saline-treated controls 6 hours after LPS challenge. Thermal injury suppressed LPS-induced IFNγ secretion by 50-60{\%}. Treatment of burned mice with glucan restored LPS-induced IFNγ levels to those observed in non-burned controls. Conclusions: Thermal injury results in suppression of both IL-12 and IFNγ secretion. Treatment of thermally injured mice with glucan restores IL-12 and IFNγ levels to those observed in non-burned mice. Glucan may serve as an adjunctive immunomodulator to prevent burn-induced immunosuppression.",
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T1 - Soluble glucan reverses burn-induced suppression of interleukin-12 and interferon gamma secretion in thermally injured mice

AU - Koutrouvelis, Aristides

AU - Lin, Cheng

AU - Sherwood, Edward

PY - 1999

Y1 - 1999

N2 - Introduction: Burn injury often induces immunosuppression, which is characterized, in part, by impairment of interleukin-12 (IL-12) and Interferon gamma (IFN-γ) secretion. Soluble β-glucans enhance host resistance to a broad spectrum of microbial pathogens. The present study was designed to determine whether glucan would reverse burn-induced suppression of IL-12 and IFN-γ secretion in vivo. Methods: Balb/C mice (4 mice/group) received 25% full-thickness flame burns under general anesthesia. Sham-burned mice served as controls. All mice were immediately resuscitated with normal saline (4 ml/ mouse IP) or saline containing glucan (40 mg/kg). On days 2 and 5 after burns, mice were challenged with E. coli endotoxin (LPS, 4 mg/kg IP) and serum samples were harvested for analysis of IL-12 and IFNγ levels using ELISA. Results: Priming of sham mice with glucan increased IL-12 secretion by 30-50% compared to saline-treated sham mice on days 2 and 5 after burn injury (see figure). Burn injury suppressed IL-12 secretion by 50-70%, yet glucan therapy restored serum IL-12 levels to those observed in non-burned control mice. Glucan priming enhanced IFNγ secretion in sham mice by 50-90% compared to saline-treated controls 6 hours after LPS challenge. Thermal injury suppressed LPS-induced IFNγ secretion by 50-60%. Treatment of burned mice with glucan restored LPS-induced IFNγ levels to those observed in non-burned controls. Conclusions: Thermal injury results in suppression of both IL-12 and IFNγ secretion. Treatment of thermally injured mice with glucan restores IL-12 and IFNγ levels to those observed in non-burned mice. Glucan may serve as an adjunctive immunomodulator to prevent burn-induced immunosuppression.

AB - Introduction: Burn injury often induces immunosuppression, which is characterized, in part, by impairment of interleukin-12 (IL-12) and Interferon gamma (IFN-γ) secretion. Soluble β-glucans enhance host resistance to a broad spectrum of microbial pathogens. The present study was designed to determine whether glucan would reverse burn-induced suppression of IL-12 and IFN-γ secretion in vivo. Methods: Balb/C mice (4 mice/group) received 25% full-thickness flame burns under general anesthesia. Sham-burned mice served as controls. All mice were immediately resuscitated with normal saline (4 ml/ mouse IP) or saline containing glucan (40 mg/kg). On days 2 and 5 after burns, mice were challenged with E. coli endotoxin (LPS, 4 mg/kg IP) and serum samples were harvested for analysis of IL-12 and IFNγ levels using ELISA. Results: Priming of sham mice with glucan increased IL-12 secretion by 30-50% compared to saline-treated sham mice on days 2 and 5 after burn injury (see figure). Burn injury suppressed IL-12 secretion by 50-70%, yet glucan therapy restored serum IL-12 levels to those observed in non-burned control mice. Glucan priming enhanced IFNγ secretion in sham mice by 50-90% compared to saline-treated controls 6 hours after LPS challenge. Thermal injury suppressed LPS-induced IFNγ secretion by 50-60%. Treatment of burned mice with glucan restored LPS-induced IFNγ levels to those observed in non-burned controls. Conclusions: Thermal injury results in suppression of both IL-12 and IFNγ secretion. Treatment of thermally injured mice with glucan restores IL-12 and IFNγ levels to those observed in non-burned mice. Glucan may serve as an adjunctive immunomodulator to prevent burn-induced immunosuppression.

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