Soluble guanylyl cyclase is a target of angiotensin II-induced nitrosative stress in a hypertensive rat model

Pierre Antoine Crassous, Samba Couloubaly, Can Huang, Zongmin Zhou, Padmamalini Baskaran, David D. Kim, Andreas Papapetropoulos, Xavier Fioramonti, Walter N. Durán, Annie Beuve

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Nitric oxide (NO) by activating soluble guanylyl cyclase (sGC) is involved in vascular homeostasis via induction of smooth muscle relaxation. In cardiovascular diseases (CVDs), endothelial dysfunction with altered vascular reactivity is mostly attributed to decreased NO bioavailability via oxidative stress. However, in several studies, relaxation to NO is only partially restored by exogenous NO donors, suggesting sGC impairment. Conflicting results have been reported regarding the nature of this impairment, ranging from decreased expression of one or both subunits of sGC to heme oxidation. We showed that sGC activity is impaired by thiol S-nitrosation. Recently, angiotensin II (ANG II) chronic treatment, which induces hypertension, was shown to generate nitrosative stress in addition to oxidative stress. We hypothesized that S-nitrosation of sGC occurs in ANG II-induced hypertension, thereby leading to desensitization of sGC to NO hence vascular dysfunction. As expected, ANG II infusion increases blood pressure, aorta remodeling, and protein S-nitrosation. Intravital microscopy indicated that cremaster arterioles are resistant to NO-induced vasodilation in vivo in anesthetized ANG II-treated rats. Concomitantly, NO-induced cGMP production decreases, which correlated with S-nitrosation of sGC in hypertensive rats. This study suggests that S-nitrosation of sGC by ANG II contributes to vascular dysfunction. This was confirmed in vitro by using A7r5 smooth muscle cells infected with adenoviruses expressing sGC or cysteine mutants: ANG II decreases NO-stimulated activity in the wild-type but not in one mutant, C516A. This result indicates that cysteine 516 of sGC mediates ANG II-induced desensitization to NO in cells.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume303
Issue number5
DOIs
StatePublished - Sep 1 2012
Externally publishedYes

Fingerprint

Angiotensin II
Nitrosation
Nitric Oxide
Blood Vessels
Cysteine
Oxidative Stress
Soluble Guanylyl Cyclase
Hypertension
Muscle Relaxation
Nitric Oxide Donors
Protein S
Arterioles
Heme
Sulfhydryl Compounds
Adenoviridae
Vasodilation
Biological Availability
Smooth Muscle Myocytes
Smooth Muscle
Aorta

Keywords

  • Hypertension
  • Nitric oxide resistance
  • Oxidative stress
  • Vascular dysfunction

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Soluble guanylyl cyclase is a target of angiotensin II-induced nitrosative stress in a hypertensive rat model. / Crassous, Pierre Antoine; Couloubaly, Samba; Huang, Can; Zhou, Zongmin; Baskaran, Padmamalini; Kim, David D.; Papapetropoulos, Andreas; Fioramonti, Xavier; Durán, Walter N.; Beuve, Annie.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 303, No. 5, 01.09.2012.

Research output: Contribution to journalArticle

Crassous, Pierre Antoine ; Couloubaly, Samba ; Huang, Can ; Zhou, Zongmin ; Baskaran, Padmamalini ; Kim, David D. ; Papapetropoulos, Andreas ; Fioramonti, Xavier ; Durán, Walter N. ; Beuve, Annie. / Soluble guanylyl cyclase is a target of angiotensin II-induced nitrosative stress in a hypertensive rat model. In: American Journal of Physiology - Heart and Circulatory Physiology. 2012 ; Vol. 303, No. 5.
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AU - Zhou, Zongmin

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AU - Kim, David D.

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