Solution structure of a cis-opened (10R)-N6-deoxyadenosine adduct of (9S,10R)-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene in a DNA duplex

David E. Volk, Varatharasa Thiviyanathan, Jeffrey S. Rice, Bruce A. Luxon, Jamshed H. Shah, Haruhiko Yagi, Jane M. Sayer, Herman J C Yeh, Donald M. Jerina, David G. Gorenstein

    Research output: Contribution to journalArticle

    34 Citations (Scopus)

    Abstract

    The solution structure of an 11-mer DNA duplex, d(CGGTCA*CGAGG)·d(CCTCGTGACCG), containing a 10R adduct at dA* that corresponds to the cis addition of the N6-amino group of dA6 to (+)-(9S,10R)-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene was studied by 2D NMR methods. The NOESY cross-peak patterns indicate that the hydrocarbon is intercalated on the 5′-side of the modified base. This observation is the same as that observed for other oligonucleotides containing (10R)-dA adducts but opposite to that observed for the corresponding (10S)-dA adducts which are intercalated on the 3′-side of the modified base. The hydrocarbon is intercalated from the major groove without significant disruption of either the anti glycosidic torsion angle of the modified residue or the base pairing of the modified residue with the complementary residue on the opposite strand. The ensemble of 10 structures determined exhibits relatively small variations (6-15°) in the characteristic hydrocarbon-base dihedral angles (α′ and β′) as well as the glycosidic torsion angle Z. These angles are similar to those in a previously determined cis-opened benzo[a]pyrene diol epoxide-(10R)-dA adduct structure. Comparison of the present structure with the cis-opened diol epoxide adduct suggests that the absence of the 7- and 8-hydroxyl groups results in more efficient stacking of the aromatic moiety with the flanking base pairs and deeper insertion of the hydrocarbon into the helix. Relative to normal B-DNA, the duplex containing the present tetrahydroepoxide adduct is unwound at the lesion site, whereas the diol epoxide adduct structure is more tightly wound than normal B-DNA. Buckling of the adducted base pair as well as the C5-G18 base pair that lies immediately above the hydrocarbon is much less severe in the present adducted structure than its cis-opened diol epoxide counterpart.

    Original languageEnglish (US)
    Pages (from-to)1410-1420
    Number of pages11
    JournalBiochemistry
    Volume42
    Issue number6
    DOIs
    StatePublished - Feb 18 2003

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    Hydrocarbons
    Epoxy Compounds
    Base Pairing
    DNA
    Torsional stress
    B-Form DNA
    Benzo(a)pyrene
    Dihedral angle
    Oligonucleotides
    Hydroxyl Radical
    Buckling
    Nuclear magnetic resonance
    2'-deoxyadenosine
    9,10-epoxy-7,8,9,10-tetrahydrobenzo(a)pyrene
    Wounds and Injuries

    ASJC Scopus subject areas

    • Biochemistry

    Cite this

    Volk, D. E., Thiviyanathan, V., Rice, J. S., Luxon, B. A., Shah, J. H., Yagi, H., ... Gorenstein, D. G. (2003). Solution structure of a cis-opened (10R)-N6-deoxyadenosine adduct of (9S,10R)-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene in a DNA duplex. Biochemistry, 42(6), 1410-1420. https://doi.org/10.1021/bi026745u

    Solution structure of a cis-opened (10R)-N6-deoxyadenosine adduct of (9S,10R)-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene in a DNA duplex. / Volk, David E.; Thiviyanathan, Varatharasa; Rice, Jeffrey S.; Luxon, Bruce A.; Shah, Jamshed H.; Yagi, Haruhiko; Sayer, Jane M.; Yeh, Herman J C; Jerina, Donald M.; Gorenstein, David G.

    In: Biochemistry, Vol. 42, No. 6, 18.02.2003, p. 1410-1420.

    Research output: Contribution to journalArticle

    Volk, DE, Thiviyanathan, V, Rice, JS, Luxon, BA, Shah, JH, Yagi, H, Sayer, JM, Yeh, HJC, Jerina, DM & Gorenstein, DG 2003, 'Solution structure of a cis-opened (10R)-N6-deoxyadenosine adduct of (9S,10R)-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene in a DNA duplex', Biochemistry, vol. 42, no. 6, pp. 1410-1420. https://doi.org/10.1021/bi026745u
    Volk, David E. ; Thiviyanathan, Varatharasa ; Rice, Jeffrey S. ; Luxon, Bruce A. ; Shah, Jamshed H. ; Yagi, Haruhiko ; Sayer, Jane M. ; Yeh, Herman J C ; Jerina, Donald M. ; Gorenstein, David G. / Solution structure of a cis-opened (10R)-N6-deoxyadenosine adduct of (9S,10R)-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene in a DNA duplex. In: Biochemistry. 2003 ; Vol. 42, No. 6. pp. 1410-1420.
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    abstract = "The solution structure of an 11-mer DNA duplex, d(CGGTCA*CGAGG)·d(CCTCGTGACCG), containing a 10R adduct at dA* that corresponds to the cis addition of the N6-amino group of dA6 to (+)-(9S,10R)-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene was studied by 2D NMR methods. The NOESY cross-peak patterns indicate that the hydrocarbon is intercalated on the 5′-side of the modified base. This observation is the same as that observed for other oligonucleotides containing (10R)-dA adducts but opposite to that observed for the corresponding (10S)-dA adducts which are intercalated on the 3′-side of the modified base. The hydrocarbon is intercalated from the major groove without significant disruption of either the anti glycosidic torsion angle of the modified residue or the base pairing of the modified residue with the complementary residue on the opposite strand. The ensemble of 10 structures determined exhibits relatively small variations (6-15°) in the characteristic hydrocarbon-base dihedral angles (α′ and β′) as well as the glycosidic torsion angle Z. These angles are similar to those in a previously determined cis-opened benzo[a]pyrene diol epoxide-(10R)-dA adduct structure. Comparison of the present structure with the cis-opened diol epoxide adduct suggests that the absence of the 7- and 8-hydroxyl groups results in more efficient stacking of the aromatic moiety with the flanking base pairs and deeper insertion of the hydrocarbon into the helix. Relative to normal B-DNA, the duplex containing the present tetrahydroepoxide adduct is unwound at the lesion site, whereas the diol epoxide adduct structure is more tightly wound than normal B-DNA. Buckling of the adducted base pair as well as the C5-G18 base pair that lies immediately above the hydrocarbon is much less severe in the present adducted structure than its cis-opened diol epoxide counterpart.",
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    AU - Volk, David E.

    AU - Thiviyanathan, Varatharasa

    AU - Rice, Jeffrey S.

    AU - Luxon, Bruce A.

    AU - Shah, Jamshed H.

    AU - Yagi, Haruhiko

    AU - Sayer, Jane M.

    AU - Yeh, Herman J C

    AU - Jerina, Donald M.

    AU - Gorenstein, David G.

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