Solution structure of the envelope protein domain III of dengue-4 virus

David E. Volk, Yi Chien Lee, Xin Li, Varatharasa Thiviyanathan, Gregory D. Gromowski, Li Li, Ashley R. Lamb, David Beasley, Alan Barrett, David G. Gorenstein

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The disease dengue (DEN) is caused by four serologically related viruses termed DEN1, DEN2, DEN3 and DEN4. The structure of the ectodomain of the envelope protein has been determined previously for DEN2 and DEN3 viruses. Using NMR spectroscopic methods, we solved the solution structure of domain III (ED3), the receptor-binding domain, of the envelope protein of DEN4 virus, human strain 703-4. The structure shows that the nine amino acid changes in ED3 that separate the sylvatic and human DEN4 strains are surface exposed. Important structural differences between DEN4-rED3 and ED3 domains of DEN2, DEN3 and other flaviviruses are discussed.

Original languageEnglish (US)
Pages (from-to)147-154
Number of pages8
JournalVirology
Volume364
Issue number1
DOIs
StatePublished - Jul 20 2007

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Dengue Virus
Viral Envelope Proteins
Viruses
Flavivirus
Dengue
Amino Acids
Proteins
Protein Domains

Keywords

  • Dengue
  • Dengue-4 virus
  • Envelope protein domain III
  • Flavivirus
  • Nuclear magnetic resonance
  • Structure

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Volk, D. E., Lee, Y. C., Li, X., Thiviyanathan, V., Gromowski, G. D., Li, L., ... Gorenstein, D. G. (2007). Solution structure of the envelope protein domain III of dengue-4 virus. Virology, 364(1), 147-154. https://doi.org/10.1016/j.virol.2007.02.023

Solution structure of the envelope protein domain III of dengue-4 virus. / Volk, David E.; Lee, Yi Chien; Li, Xin; Thiviyanathan, Varatharasa; Gromowski, Gregory D.; Li, Li; Lamb, Ashley R.; Beasley, David; Barrett, Alan; Gorenstein, David G.

In: Virology, Vol. 364, No. 1, 20.07.2007, p. 147-154.

Research output: Contribution to journalArticle

Volk, DE, Lee, YC, Li, X, Thiviyanathan, V, Gromowski, GD, Li, L, Lamb, AR, Beasley, D, Barrett, A & Gorenstein, DG 2007, 'Solution structure of the envelope protein domain III of dengue-4 virus', Virology, vol. 364, no. 1, pp. 147-154. https://doi.org/10.1016/j.virol.2007.02.023
Volk DE, Lee YC, Li X, Thiviyanathan V, Gromowski GD, Li L et al. Solution structure of the envelope protein domain III of dengue-4 virus. Virology. 2007 Jul 20;364(1):147-154. https://doi.org/10.1016/j.virol.2007.02.023
Volk, David E. ; Lee, Yi Chien ; Li, Xin ; Thiviyanathan, Varatharasa ; Gromowski, Gregory D. ; Li, Li ; Lamb, Ashley R. ; Beasley, David ; Barrett, Alan ; Gorenstein, David G. / Solution structure of the envelope protein domain III of dengue-4 virus. In: Virology. 2007 ; Vol. 364, No. 1. pp. 147-154.
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