Somatic cell expression of the c-mos protein

N. K. Herzog, L. S. Ramagli, R. B. Arlinghaus

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The c-mos protein has been found to be enriched in germ cells of male mice, as described in a recent report from this laboratory (Herzog et al., Oncogene 3, 225, 1988). We report on further studies which indicate that the c-mos protein (a 41 to 43 kDa protein termed p43(c-mos)) is expressed in somatic tissues of mice and in cells grown in culture. In testes of mice, germ cell fractions have increased levels of p43(c-mos) relative to other cells of the testes. However, non-germ cells harbor significant levels of p43(c-mos), as judged by comparison of testes from normal mice to those with mutations that affect the germ cell content of the testes. Thus, homozygous S1, at, and the W/W(v) mutant mice are sterile due to severe deficiencies of germ cells. Such mice had only an estimated 50%-60% reduction in p43(c-mos) as judged by western immunoblotting using two different site-directed anti-mos antibodies. Similary, X/X-sex reversed mice in which germ cells die after 10 days of age had only an 85% reduction of p43(c-mos) in mice 35 days of age. Thus, the germ cell content of testes did not correlate with p43(c-mos) levels in this tissue. Direct analyses of non-germ cells derived from mouse testes confirmed these findings, since Sertoli and Leydig cell lines grown in culture expressed p43(c-mos). In addition, tissues such as kidney, liver, spleen and brain were found to contain p43(c-mos). Surprisingly, mouse NIH3T3 cells were found to express significant levels of the c-mos protein based upon immunoblotting and one-dimensional peptide mapping experiments performed with both anti-mos antibodies. The concentration of the c-mos protein was not affected by expression of viral mos proteins. We conclude that the c-mos protein is enriched in male germ cells, but p43(c-mos) is also expressed in significant amounts in somatic tissues and in fibroblastic cells grown in culture.

Original languageEnglish (US)
Pages (from-to)1307-1315
Number of pages9
JournalOncogene
Volume4
Issue number11
StatePublished - 1989
Externally publishedYes

Fingerprint

Proto-Oncogene Proteins c-mos
Germ Cells
Testis
Anti-Idiotypic Antibodies
Peptide Mapping
Leydig Cells
Sertoli Cells
Viral Proteins
Oncogenes
Immunoblotting

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Herzog, N. K., Ramagli, L. S., & Arlinghaus, R. B. (1989). Somatic cell expression of the c-mos protein. Oncogene, 4(11), 1307-1315.

Somatic cell expression of the c-mos protein. / Herzog, N. K.; Ramagli, L. S.; Arlinghaus, R. B.

In: Oncogene, Vol. 4, No. 11, 1989, p. 1307-1315.

Research output: Contribution to journalArticle

Herzog, NK, Ramagli, LS & Arlinghaus, RB 1989, 'Somatic cell expression of the c-mos protein', Oncogene, vol. 4, no. 11, pp. 1307-1315.
Herzog NK, Ramagli LS, Arlinghaus RB. Somatic cell expression of the c-mos protein. Oncogene. 1989;4(11):1307-1315.
Herzog, N. K. ; Ramagli, L. S. ; Arlinghaus, R. B. / Somatic cell expression of the c-mos protein. In: Oncogene. 1989 ; Vol. 4, No. 11. pp. 1307-1315.
@article{87536ed0ba1948c1865939212b6f31c6,
title = "Somatic cell expression of the c-mos protein",
abstract = "The c-mos protein has been found to be enriched in germ cells of male mice, as described in a recent report from this laboratory (Herzog et al., Oncogene 3, 225, 1988). We report on further studies which indicate that the c-mos protein (a 41 to 43 kDa protein termed p43(c-mos)) is expressed in somatic tissues of mice and in cells grown in culture. In testes of mice, germ cell fractions have increased levels of p43(c-mos) relative to other cells of the testes. However, non-germ cells harbor significant levels of p43(c-mos), as judged by comparison of testes from normal mice to those with mutations that affect the germ cell content of the testes. Thus, homozygous S1, at, and the W/W(v) mutant mice are sterile due to severe deficiencies of germ cells. Such mice had only an estimated 50{\%}-60{\%} reduction in p43(c-mos) as judged by western immunoblotting using two different site-directed anti-mos antibodies. Similary, X/X-sex reversed mice in which germ cells die after 10 days of age had only an 85{\%} reduction of p43(c-mos) in mice 35 days of age. Thus, the germ cell content of testes did not correlate with p43(c-mos) levels in this tissue. Direct analyses of non-germ cells derived from mouse testes confirmed these findings, since Sertoli and Leydig cell lines grown in culture expressed p43(c-mos). In addition, tissues such as kidney, liver, spleen and brain were found to contain p43(c-mos). Surprisingly, mouse NIH3T3 cells were found to express significant levels of the c-mos protein based upon immunoblotting and one-dimensional peptide mapping experiments performed with both anti-mos antibodies. The concentration of the c-mos protein was not affected by expression of viral mos proteins. We conclude that the c-mos protein is enriched in male germ cells, but p43(c-mos) is also expressed in significant amounts in somatic tissues and in fibroblastic cells grown in culture.",
author = "Herzog, {N. K.} and Ramagli, {L. S.} and Arlinghaus, {R. B.}",
year = "1989",
language = "English (US)",
volume = "4",
pages = "1307--1315",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "11",

}

TY - JOUR

T1 - Somatic cell expression of the c-mos protein

AU - Herzog, N. K.

AU - Ramagli, L. S.

AU - Arlinghaus, R. B.

PY - 1989

Y1 - 1989

N2 - The c-mos protein has been found to be enriched in germ cells of male mice, as described in a recent report from this laboratory (Herzog et al., Oncogene 3, 225, 1988). We report on further studies which indicate that the c-mos protein (a 41 to 43 kDa protein termed p43(c-mos)) is expressed in somatic tissues of mice and in cells grown in culture. In testes of mice, germ cell fractions have increased levels of p43(c-mos) relative to other cells of the testes. However, non-germ cells harbor significant levels of p43(c-mos), as judged by comparison of testes from normal mice to those with mutations that affect the germ cell content of the testes. Thus, homozygous S1, at, and the W/W(v) mutant mice are sterile due to severe deficiencies of germ cells. Such mice had only an estimated 50%-60% reduction in p43(c-mos) as judged by western immunoblotting using two different site-directed anti-mos antibodies. Similary, X/X-sex reversed mice in which germ cells die after 10 days of age had only an 85% reduction of p43(c-mos) in mice 35 days of age. Thus, the germ cell content of testes did not correlate with p43(c-mos) levels in this tissue. Direct analyses of non-germ cells derived from mouse testes confirmed these findings, since Sertoli and Leydig cell lines grown in culture expressed p43(c-mos). In addition, tissues such as kidney, liver, spleen and brain were found to contain p43(c-mos). Surprisingly, mouse NIH3T3 cells were found to express significant levels of the c-mos protein based upon immunoblotting and one-dimensional peptide mapping experiments performed with both anti-mos antibodies. The concentration of the c-mos protein was not affected by expression of viral mos proteins. We conclude that the c-mos protein is enriched in male germ cells, but p43(c-mos) is also expressed in significant amounts in somatic tissues and in fibroblastic cells grown in culture.

AB - The c-mos protein has been found to be enriched in germ cells of male mice, as described in a recent report from this laboratory (Herzog et al., Oncogene 3, 225, 1988). We report on further studies which indicate that the c-mos protein (a 41 to 43 kDa protein termed p43(c-mos)) is expressed in somatic tissues of mice and in cells grown in culture. In testes of mice, germ cell fractions have increased levels of p43(c-mos) relative to other cells of the testes. However, non-germ cells harbor significant levels of p43(c-mos), as judged by comparison of testes from normal mice to those with mutations that affect the germ cell content of the testes. Thus, homozygous S1, at, and the W/W(v) mutant mice are sterile due to severe deficiencies of germ cells. Such mice had only an estimated 50%-60% reduction in p43(c-mos) as judged by western immunoblotting using two different site-directed anti-mos antibodies. Similary, X/X-sex reversed mice in which germ cells die after 10 days of age had only an 85% reduction of p43(c-mos) in mice 35 days of age. Thus, the germ cell content of testes did not correlate with p43(c-mos) levels in this tissue. Direct analyses of non-germ cells derived from mouse testes confirmed these findings, since Sertoli and Leydig cell lines grown in culture expressed p43(c-mos). In addition, tissues such as kidney, liver, spleen and brain were found to contain p43(c-mos). Surprisingly, mouse NIH3T3 cells were found to express significant levels of the c-mos protein based upon immunoblotting and one-dimensional peptide mapping experiments performed with both anti-mos antibodies. The concentration of the c-mos protein was not affected by expression of viral mos proteins. We conclude that the c-mos protein is enriched in male germ cells, but p43(c-mos) is also expressed in significant amounts in somatic tissues and in fibroblastic cells grown in culture.

UR - http://www.scopus.com/inward/record.url?scp=0024449644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024449644&partnerID=8YFLogxK

M3 - Article

VL - 4

SP - 1307

EP - 1315

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 11

ER -