Source and amount of dietary nonspecific nitrogen in relation to whole-body leucine, phenylalanine, and tyrosine kinetics in young men

Takeyuki Hiramatsu, Joaquin Cortiella, J. Sérgio Marchini, Thomas E. Chapman, Vernon R. Young

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We studied the effects of amount and source of nonspecific nitrogen (NSN) on the oxidation of leucine and hydroxylation of phenylalanine. In phase 1, seven adult males received for 6 d diets providing indispensable amino acid intakes to meet the 1985 FAO/WHO/UNU (FAO) requirements or our proposed requirement values (MIT). During one diet period with each diet, the NSN of the basal diets (total nitrogen intake: 107 mg N-kg-1·d-1) was increased to a total of 160 mg N·kg-1·d-1. On the morning of day 7, an 8-h constant intravenous tracer-infusion protocol (3-h fast; 5-h fed state) was conducted with L-[1-13C]leucine, L-[ring-2H5]phenylalanine, and L-[3,3,2H2]-tyrosine as tracers. In phase 2, six subjects were given three diets for 6 d, supplying 107 mg N·kg-1·d-1; NSN was a mixture of dispensable amino acids in which glutamine accounted for 0%, 12.5%, and 100% of total NSN. Leucine oxidation and phenylalanine hydroxylation rates and whole-body leucine and phenylalanine balances were unaffected by addition of supplemental NSN to the diets in phase 1 or by amino acid source of NSN in phase 2. Leucine and phenylalanine balances were lower (P < 0.05) for FAO compared with MIT diets.

Original languageEnglish (US)
Pages (from-to)1347-1355
Number of pages9
JournalAmerican Journal of Clinical Nutrition
Volume59
Issue number6
StatePublished - Jun 1994
Externally publishedYes

Fingerprint

Phenylalanine
phenylalanine
Leucine
leucine
Tyrosine
tyrosine
Nitrogen
Diet
kinetics
nitrogen
diet
hydroxylation
Hydroxylation
Amino Acids
amino acids
tracer techniques
oxidation
Glutamine
Intravenous Infusions
glutamine

Keywords

  • Amino acids
  • Kinetics
  • Leucine
  • Phenylalanine
  • Requirements

ASJC Scopus subject areas

  • Food Science
  • Medicine (miscellaneous)

Cite this

Source and amount of dietary nonspecific nitrogen in relation to whole-body leucine, phenylalanine, and tyrosine kinetics in young men. / Hiramatsu, Takeyuki; Cortiella, Joaquin; Marchini, J. Sérgio; Chapman, Thomas E.; Young, Vernon R.

In: American Journal of Clinical Nutrition, Vol. 59, No. 6, 06.1994, p. 1347-1355.

Research output: Contribution to journalArticle

Hiramatsu, Takeyuki ; Cortiella, Joaquin ; Marchini, J. Sérgio ; Chapman, Thomas E. ; Young, Vernon R. / Source and amount of dietary nonspecific nitrogen in relation to whole-body leucine, phenylalanine, and tyrosine kinetics in young men. In: American Journal of Clinical Nutrition. 1994 ; Vol. 59, No. 6. pp. 1347-1355.
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