Soy-dairy protein blend or whey protein isolate ingestion induces similar postexercise muscle mechanistic target of rapamycin complex 1 signaling and protein synthesis responses in older men

Michael S. Borack, Paul T. Reidy, Syed H. Husaini, Melissa M. Markofski, Rachel Deer, Abigail B. Richison, Bradley S. Lambert, Mark B. Cope, Ratna Mukherjea, Kristofer Jennings, Elena Volpi, Blake Rasmussen

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Previous work demonstrated that a soy-dairy protein blend (PB) prolongs hyperaminoacidemia and muscle protein synthesis in young adults after resistance exercise. Objective: We investigated the effect of PB in older adults. We hypothesized that PB would prolong hyperaminoacidemia, enhancing mechanistic target of rapamycin complex 1 (mTORC1) signaling and muscle protein anabolism compared with a whey protein isolate (WPI). Methods: This double-blind, randomized controlled trial studied men 55-75 y of age. Subjects consumed 30 g protein from WPI or PB (25% soy, 25% whey, and 50% casein) 1 h after leg extension exercise (8 sets of 10 repetitions at 70% one-repetition maximum). Blood and muscle amino acid concentrations and basal and postexercise muscle protein turnover were measured by using stable isotopic methods. Muscle mTORC1 signaling was assessed by immunoblotting. Results: Both groups increased amino acid concentrations (P < 0.05) and mTORC1 signaling after protein ingestion (P < 0.05). Postexercise fractional synthesis rate (FSR; P ≥ 0.05), fractional breakdown rate (FBR; P ≥ 0.05), and net balance (P = 0.08) did not differ between groups. WPI increased FSR by 67% (mean ± SEM: rest: 0.05% ± 0.01%; postexercise: 0.09% ± 0.01%; P < 0.05), decreased FBR by 46% (rest: 0.17% ± 0.01%; postexercise: 0.09% ± 0.03%; P < 0.05), andmade net balance less negative (P < 0.05). PB ingestion did not increase FSR (rest: 0.07%±0.03%; postexercise: 0.09% ± 0.01%; P ≥ 0.05), tended to decrease FBR by 42% (rest: 0.25% ± 0.08%; postexercise: 0.15% ± 0.08%; P = 0.08), and made net balance less negative (P < 0.05). Within-group percentage of change differences were not different between groups for FSR, FBR, or net balance (P ≥ 0.05). Conclusions: WPI and PB ingestion after exercise in older men induced similar responses in hyperaminoacidemia, mTORC1 signaling, muscle protein synthesis, and breakdown. These data add new evidence for the use of whey or soydairy PBs as targeted nutritional interventions to counteract sarcopenia.

Original languageEnglish (US)
Pages (from-to)2468-2475
Number of pages8
JournalJournal of Nutrition
Volume146
Issue number12
DOIs
StatePublished - Dec 1 2016

Fingerprint

Soybean Proteins
Eating
Muscle Proteins
Muscles
Proteins
Exercise
Sarcopenia
Amino Acids
Caseins
mechanistic target of rapamycin complex 1
Whey Proteins
Double-Blind Method
Immunoblotting
Young Adult
Leg
Randomized Controlled Trials

Keywords

  • Aging
  • Leucine
  • Muscle protein turnover
  • Protein supplementation
  • Sarcopenia

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Soy-dairy protein blend or whey protein isolate ingestion induces similar postexercise muscle mechanistic target of rapamycin complex 1 signaling and protein synthesis responses in older men. / Borack, Michael S.; Reidy, Paul T.; Husaini, Syed H.; Markofski, Melissa M.; Deer, Rachel; Richison, Abigail B.; Lambert, Bradley S.; Cope, Mark B.; Mukherjea, Ratna; Jennings, Kristofer; Volpi, Elena; Rasmussen, Blake.

In: Journal of Nutrition, Vol. 146, No. 12, 01.12.2016, p. 2468-2475.

Research output: Contribution to journalArticle

Borack, Michael S. ; Reidy, Paul T. ; Husaini, Syed H. ; Markofski, Melissa M. ; Deer, Rachel ; Richison, Abigail B. ; Lambert, Bradley S. ; Cope, Mark B. ; Mukherjea, Ratna ; Jennings, Kristofer ; Volpi, Elena ; Rasmussen, Blake. / Soy-dairy protein blend or whey protein isolate ingestion induces similar postexercise muscle mechanistic target of rapamycin complex 1 signaling and protein synthesis responses in older men. In: Journal of Nutrition. 2016 ; Vol. 146, No. 12. pp. 2468-2475.
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title = "Soy-dairy protein blend or whey protein isolate ingestion induces similar postexercise muscle mechanistic target of rapamycin complex 1 signaling and protein synthesis responses in older men",
abstract = "Background: Previous work demonstrated that a soy-dairy protein blend (PB) prolongs hyperaminoacidemia and muscle protein synthesis in young adults after resistance exercise. Objective: We investigated the effect of PB in older adults. We hypothesized that PB would prolong hyperaminoacidemia, enhancing mechanistic target of rapamycin complex 1 (mTORC1) signaling and muscle protein anabolism compared with a whey protein isolate (WPI). Methods: This double-blind, randomized controlled trial studied men 55-75 y of age. Subjects consumed 30 g protein from WPI or PB (25{\%} soy, 25{\%} whey, and 50{\%} casein) 1 h after leg extension exercise (8 sets of 10 repetitions at 70{\%} one-repetition maximum). Blood and muscle amino acid concentrations and basal and postexercise muscle protein turnover were measured by using stable isotopic methods. Muscle mTORC1 signaling was assessed by immunoblotting. Results: Both groups increased amino acid concentrations (P < 0.05) and mTORC1 signaling after protein ingestion (P < 0.05). Postexercise fractional synthesis rate (FSR; P ≥ 0.05), fractional breakdown rate (FBR; P ≥ 0.05), and net balance (P = 0.08) did not differ between groups. WPI increased FSR by 67{\%} (mean ± SEM: rest: 0.05{\%} ± 0.01{\%}; postexercise: 0.09{\%} ± 0.01{\%}; P < 0.05), decreased FBR by 46{\%} (rest: 0.17{\%} ± 0.01{\%}; postexercise: 0.09{\%} ± 0.03{\%}; P < 0.05), andmade net balance less negative (P < 0.05). PB ingestion did not increase FSR (rest: 0.07{\%}±0.03{\%}; postexercise: 0.09{\%} ± 0.01{\%}; P ≥ 0.05), tended to decrease FBR by 42{\%} (rest: 0.25{\%} ± 0.08{\%}; postexercise: 0.15{\%} ± 0.08{\%}; P = 0.08), and made net balance less negative (P < 0.05). Within-group percentage of change differences were not different between groups for FSR, FBR, or net balance (P ≥ 0.05). Conclusions: WPI and PB ingestion after exercise in older men induced similar responses in hyperaminoacidemia, mTORC1 signaling, muscle protein synthesis, and breakdown. These data add new evidence for the use of whey or soydairy PBs as targeted nutritional interventions to counteract sarcopenia.",
keywords = "Aging, Leucine, Muscle protein turnover, Protein supplementation, Sarcopenia",
author = "Borack, {Michael S.} and Reidy, {Paul T.} and Husaini, {Syed H.} and Markofski, {Melissa M.} and Rachel Deer and Richison, {Abigail B.} and Lambert, {Bradley S.} and Cope, {Mark B.} and Ratna Mukherjea and Kristofer Jennings and Elena Volpi and Blake Rasmussen",
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pages = "2468--2475",
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TY - JOUR

T1 - Soy-dairy protein blend or whey protein isolate ingestion induces similar postexercise muscle mechanistic target of rapamycin complex 1 signaling and protein synthesis responses in older men

AU - Borack, Michael S.

AU - Reidy, Paul T.

AU - Husaini, Syed H.

AU - Markofski, Melissa M.

AU - Deer, Rachel

AU - Richison, Abigail B.

AU - Lambert, Bradley S.

AU - Cope, Mark B.

AU - Mukherjea, Ratna

AU - Jennings, Kristofer

AU - Volpi, Elena

AU - Rasmussen, Blake

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Background: Previous work demonstrated that a soy-dairy protein blend (PB) prolongs hyperaminoacidemia and muscle protein synthesis in young adults after resistance exercise. Objective: We investigated the effect of PB in older adults. We hypothesized that PB would prolong hyperaminoacidemia, enhancing mechanistic target of rapamycin complex 1 (mTORC1) signaling and muscle protein anabolism compared with a whey protein isolate (WPI). Methods: This double-blind, randomized controlled trial studied men 55-75 y of age. Subjects consumed 30 g protein from WPI or PB (25% soy, 25% whey, and 50% casein) 1 h after leg extension exercise (8 sets of 10 repetitions at 70% one-repetition maximum). Blood and muscle amino acid concentrations and basal and postexercise muscle protein turnover were measured by using stable isotopic methods. Muscle mTORC1 signaling was assessed by immunoblotting. Results: Both groups increased amino acid concentrations (P < 0.05) and mTORC1 signaling after protein ingestion (P < 0.05). Postexercise fractional synthesis rate (FSR; P ≥ 0.05), fractional breakdown rate (FBR; P ≥ 0.05), and net balance (P = 0.08) did not differ between groups. WPI increased FSR by 67% (mean ± SEM: rest: 0.05% ± 0.01%; postexercise: 0.09% ± 0.01%; P < 0.05), decreased FBR by 46% (rest: 0.17% ± 0.01%; postexercise: 0.09% ± 0.03%; P < 0.05), andmade net balance less negative (P < 0.05). PB ingestion did not increase FSR (rest: 0.07%±0.03%; postexercise: 0.09% ± 0.01%; P ≥ 0.05), tended to decrease FBR by 42% (rest: 0.25% ± 0.08%; postexercise: 0.15% ± 0.08%; P = 0.08), and made net balance less negative (P < 0.05). Within-group percentage of change differences were not different between groups for FSR, FBR, or net balance (P ≥ 0.05). Conclusions: WPI and PB ingestion after exercise in older men induced similar responses in hyperaminoacidemia, mTORC1 signaling, muscle protein synthesis, and breakdown. These data add new evidence for the use of whey or soydairy PBs as targeted nutritional interventions to counteract sarcopenia.

AB - Background: Previous work demonstrated that a soy-dairy protein blend (PB) prolongs hyperaminoacidemia and muscle protein synthesis in young adults after resistance exercise. Objective: We investigated the effect of PB in older adults. We hypothesized that PB would prolong hyperaminoacidemia, enhancing mechanistic target of rapamycin complex 1 (mTORC1) signaling and muscle protein anabolism compared with a whey protein isolate (WPI). Methods: This double-blind, randomized controlled trial studied men 55-75 y of age. Subjects consumed 30 g protein from WPI or PB (25% soy, 25% whey, and 50% casein) 1 h after leg extension exercise (8 sets of 10 repetitions at 70% one-repetition maximum). Blood and muscle amino acid concentrations and basal and postexercise muscle protein turnover were measured by using stable isotopic methods. Muscle mTORC1 signaling was assessed by immunoblotting. Results: Both groups increased amino acid concentrations (P < 0.05) and mTORC1 signaling after protein ingestion (P < 0.05). Postexercise fractional synthesis rate (FSR; P ≥ 0.05), fractional breakdown rate (FBR; P ≥ 0.05), and net balance (P = 0.08) did not differ between groups. WPI increased FSR by 67% (mean ± SEM: rest: 0.05% ± 0.01%; postexercise: 0.09% ± 0.01%; P < 0.05), decreased FBR by 46% (rest: 0.17% ± 0.01%; postexercise: 0.09% ± 0.03%; P < 0.05), andmade net balance less negative (P < 0.05). PB ingestion did not increase FSR (rest: 0.07%±0.03%; postexercise: 0.09% ± 0.01%; P ≥ 0.05), tended to decrease FBR by 42% (rest: 0.25% ± 0.08%; postexercise: 0.15% ± 0.08%; P = 0.08), and made net balance less negative (P < 0.05). Within-group percentage of change differences were not different between groups for FSR, FBR, or net balance (P ≥ 0.05). Conclusions: WPI and PB ingestion after exercise in older men induced similar responses in hyperaminoacidemia, mTORC1 signaling, muscle protein synthesis, and breakdown. These data add new evidence for the use of whey or soydairy PBs as targeted nutritional interventions to counteract sarcopenia.

KW - Aging

KW - Leucine

KW - Muscle protein turnover

KW - Protein supplementation

KW - Sarcopenia

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