Species and stage specificity of Leishmania donovani antigens

A. Clinton White, C. Hanham

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Monoclonal antibodies D2 and D13 were produced in mice using Leishmania donovani promastigote membrane fractions. To study the species and stage specificity of the antigens recognized by these antibodies, we examined amastigotes prepared in vitro and cultured promastigotes by indirect immunofluorescence with monoclonal antibodies D2 and D13. Monoclonal antibody D2 showed weak reactivity for 9 of 9 strains of L. donovani complex promastigotes and 8 of 9 amastigotes. In contrast, only 2 of 22 strains from other complexes yielded equivocal reactions. Monoclonal antibody D13, however, had much broader reactivity. D13 reacted with all the promastigotes and amastigotes of L. donovani complex isolates as well as with 10 of 22 promastigotes and 8 of 13 amastigotes from other complexes. The high degree of species specificity seen with monoclonal antibody D2 provides a rationale for further study of this antibody and its purified antigen for parasite identification and serodiagnosis of visceral leishmaniasis. The strong fluorescent signal noted with D13 and the presence of the D13 epitope on all L. donovani complex parasites supports studies on its role as an antigen in immunoprophylaxis of visceral leishmaniasis.

Original languageEnglish (US)
Pages (from-to)142-150
Number of pages9
JournalJournal of Parasitology
Volume77
Issue number1
StatePublished - 1991
Externally publishedYes

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Species Specificity
Leishmania donovani
promastigotes
amastigotes
antigen
antibody
monoclonal antibodies
Monoclonal Antibodies
antigens
Antigens
visceral leishmaniasis
Visceral Leishmaniasis
leishmaniasis
Parasites
parasites
antibodies
parasite
Antibodies
serodiagnosis
Serologic Tests

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Species and stage specificity of Leishmania donovani antigens. / White, A. Clinton; Hanham, C.

In: Journal of Parasitology, Vol. 77, No. 1, 1991, p. 142-150.

Research output: Contribution to journalArticle

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