Species distribution and antifungal susceptibility profile off Candida isolates from bloodstream infections in Lima, Peru

B. Bustamante, M. A. Martins, L. X. Bonfietti, M. W. Szeszs, J. Jacobs, C. Garcia, M. S C Melhem

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Yeast identification and in vitro susceptibility testing provide helpful information of appropriate administration of antifungal treatments; however, few reports from the Latin American region have been published. The aim of this study was to identify the species present in isolates from bloodstream infections diagnosed in nine hospitals in Lima, Peru and to determine their in vitro susceptibility to four antifungal drugs. We tested and identified 153 isolates collected between October 2009 and August 2011 using standard methods. PCR and PCR-R LP assays were performed to distinguish Candida albicans from Candida dubliniensis and to identify species of the Candida parapsilosis and Candida glabrata complexes. Antifungal susceptibility testing of fluconazole, anidulafungin and voriconazole was performed using the CSLI M27-A3 method, and amphotericin B susceptibility was determined using the Etest method. The most frequently isolated species were: C. albicans (61; 39.9 %), C. parapsilosis (43; 28.1 %), C. tropicalis (36; 23.5%) and C. glabrata (8; 5.2 %). The overall susceptibility rates were 98.0 %, 98.7 %, 98.0% and 97.4% of amphotericin B, fluconazole, voriconazole and anidulafungin, respectively. No isolate was resistant to more than one drug. These results showed that the rate of resistance to four antifungal drugs was low among Candida bloodstream isolates in Lima, Peru.

Original languageEnglish (US)
Pages (from-to)855-860
Number of pages6
JournalJournal of Medical Microbiology
Volume63
Issue numberPART 6
DOIs
StatePublished - 2014
Externally publishedYes

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anidulafungin
Peru
Candida
Candida glabrata
Fluconazole
Amphotericin B
Candida albicans
Disk Diffusion Antimicrobial Tests
Infection
Pharmaceutical Preparations
Polymerase Chain Reaction
Yeasts
Voriconazole
In Vitro Techniques

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Bustamante, B., Martins, M. A., Bonfietti, L. X., Szeszs, M. W., Jacobs, J., Garcia, C., & Melhem, M. S. C. (2014). Species distribution and antifungal susceptibility profile off Candida isolates from bloodstream infections in Lima, Peru. Journal of Medical Microbiology, 63(PART 6), 855-860. https://doi.org/10.1099/jmm.0.071167-0

Species distribution and antifungal susceptibility profile off Candida isolates from bloodstream infections in Lima, Peru. / Bustamante, B.; Martins, M. A.; Bonfietti, L. X.; Szeszs, M. W.; Jacobs, J.; Garcia, C.; Melhem, M. S C.

In: Journal of Medical Microbiology, Vol. 63, No. PART 6, 2014, p. 855-860.

Research output: Contribution to journalArticle

Bustamante, B, Martins, MA, Bonfietti, LX, Szeszs, MW, Jacobs, J, Garcia, C & Melhem, MSC 2014, 'Species distribution and antifungal susceptibility profile off Candida isolates from bloodstream infections in Lima, Peru', Journal of Medical Microbiology, vol. 63, no. PART 6, pp. 855-860. https://doi.org/10.1099/jmm.0.071167-0
Bustamante, B. ; Martins, M. A. ; Bonfietti, L. X. ; Szeszs, M. W. ; Jacobs, J. ; Garcia, C. ; Melhem, M. S C. / Species distribution and antifungal susceptibility profile off Candida isolates from bloodstream infections in Lima, Peru. In: Journal of Medical Microbiology. 2014 ; Vol. 63, No. PART 6. pp. 855-860.
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