Spectroscopic studies of the effect of the metal ions on the structure of mucin

Yunlan Su, Yizhuang Xu, Limin Yang, Shifu Weng, R. D. Soloway, Dujin Wang, Jinguang Wu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In this study the binding characteristics of mucin to calcium ion was examined by Fourier Transform Infrared (FT-IR), FT-Raman, Transmission Electron Microscope (TEM), and Dynamic Light Scattering (DLS) methods. The binding site of the interaction between mucin and calcium ions could be confirmed by FT-IR and FT-Raman techniques. When the concentration of Ca2+ is relatively low, Ca2+ prefers to coordinate with the carbohydrate moiety of mucins. When the concentration of Ca2+ is high, Ca2+ will also interact with the protein moiety of mucins. The morphology and the size of CaCl2-mucin solution could be obtained by TEM and DLS methods, respectively. The hydrodynamic radius of CaCl2-mucin mixture decreases compared with pure mucin solution, which may result from that Ca2+ induces a contraction or folding of mucin chains to form a more compact configuration. The activity of the cations in modifying the structure of mucin may be of great importance for the biological function in normal and disease states.

Original languageEnglish (US)
Pages (from-to)8-13
Number of pages6
JournalJournal of Molecular Structure
Volume920
Issue number1-3
DOIs
StatePublished - Feb 28 2009

Fingerprint

Mucins
Metal ions
Dynamic light scattering
Fourier transforms
Electron microscopes
Ions
Calcium
Infrared radiation
Cations
Hydrodynamics
Binding Sites
Carbohydrates

Keywords

  • Binding
  • Metal ion
  • Mucin
  • Spectroscopic study

ASJC Scopus subject areas

  • Spectroscopy
  • Analytical Chemistry
  • Inorganic Chemistry
  • Organic Chemistry

Cite this

Spectroscopic studies of the effect of the metal ions on the structure of mucin. / Su, Yunlan; Xu, Yizhuang; Yang, Limin; Weng, Shifu; Soloway, R. D.; Wang, Dujin; Wu, Jinguang.

In: Journal of Molecular Structure, Vol. 920, No. 1-3, 28.02.2009, p. 8-13.

Research output: Contribution to journalArticle

Su, Yunlan ; Xu, Yizhuang ; Yang, Limin ; Weng, Shifu ; Soloway, R. D. ; Wang, Dujin ; Wu, Jinguang. / Spectroscopic studies of the effect of the metal ions on the structure of mucin. In: Journal of Molecular Structure. 2009 ; Vol. 920, No. 1-3. pp. 8-13.
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