Spinal cord ventral horns and lymphoid organ involvement in powassan virus infection in a mouse model

Rodrigo I. Santos, Meghan Hermance, Benjamin Gelman, Saravanan Thangamani

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Powassan virus (POWV) belongs to the family Flaviviridae and is a member of the tick-borne encephalitis serogroup. Transmission of POWV from infected ticks to humans has been documented in the USA, Canada, and Russia, causing fatal encephalitis in 10% of human cases and significant neurological sequelae in survivors. We used C57BL/6 mice to investigate POWV infection and pathogenesis. After footpad inoculation, infected animals exhibited rapid disease progression and 100% mortality. Immunohistochemistry and immunofluorescence revealed a very strong neuronal tropism of POWV infection. The central nervous system infection appeared as a meningoencephalitis with perivascular mononuclear infiltration and microglial activation in the brain, and a poliomyelitis-like syndrome with high level of POWV antigen at the ventral horn of the spinal cord. Pathological studies also revealed substantial infection of splenic macrophages by POWV, which suggests that the spleen plays a more important role in pathogenesis than previously realized. This report provides a detailed description of the neuroanatomical distribution of the lesions produced by POWV infection in C57BL/6 mice.

Original languageEnglish (US)
Article number220
JournalViruses
Volume8
Issue number8
DOIs
StatePublished - Aug 12 2016

Fingerprint

Tick-Borne Encephalitis Viruses
Virus Diseases
Inbred C57BL Mouse
Flaviviridae
Tick-Borne Encephalitis
Central Nervous System Infections
Meningoencephalitis
Tropism
Russia
Poliomyelitis
Ticks
Encephalitis
Spinal Cord Ventral Horn
Canada
Fluorescent Antibody Technique
Survivors
Disease Progression
Spleen
Immunohistochemistry
Macrophages

Keywords

  • Lymphoid organs infection
  • Poliomyelitis-like syndrome
  • Powassan virus

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Spinal cord ventral horns and lymphoid organ involvement in powassan virus infection in a mouse model. / Santos, Rodrigo I.; Hermance, Meghan; Gelman, Benjamin; Thangamani, Saravanan.

In: Viruses, Vol. 8, No. 8, 220, 12.08.2016.

Research output: Contribution to journalArticle

Santos, Rodrigo I. ; Hermance, Meghan ; Gelman, Benjamin ; Thangamani, Saravanan. / Spinal cord ventral horns and lymphoid organ involvement in powassan virus infection in a mouse model. In: Viruses. 2016 ; Vol. 8, No. 8.
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