Abstract
Purpose: To investigate the prevalence of spontaneously developed tail swellings (SDTS) in human sperm samples that are commonly encountered in the laboratory, and their influence on the hypo-osmotic swelling test (HOS-test). Methods: Ejaculated, epididymal, and testicular sperm were evaluated for SDTS. Further, HOS-test scores were compared with those of vital stains using column washed sperm maintained in the laboratory. Results: SDTS, at <10%, was present in all types of sperm samples. The highest and lowest occurrences of SDTS were found in cryopreserved sperm, and column-washed sperm respectively. SDTS can inflate the HOS-test score, and so lower the accuracy of the HOS-test. However, the HOS-test efficiency can be improved by assessing SDTS in the sample. Conclusion: HOS-test and vital stain cannot be used interchangeably in all circumstances for sperm viability determination. The accuracy of the HOS-test can be enhanced by incorporating SDTS as a correction factor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 83-86 |
| Number of pages | 4 |
| Journal | Journal of Assisted Reproduction and Genetics |
| Volume | 27 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Feb 2010 |
Keywords
- HOS-Test
- Spontaneously developed tail swellings
- Vital stain
ASJC Scopus subject areas
- Reproductive Medicine
- Genetics
- Obstetrics and Gynecology
- Developmental Biology
- Genetics(clinical)
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