Spot-scan imaging of ice-embedded catalase crystal on a slow-scan CCD camera in a 400-kV electron cryomicroscope

Wah Chiu, Michael Sherman, Jaap Brink

Research output: Contribution to conferencePaper

Abstract

In protein electron crystallography, both low dose electron diffraction patterns and images are needed to provide accurate amplitudes and phases respectively for a 3-dimensional reconstruction. A limitation of the slow scan CCD camera for recording electron images of protein crystals arises from the relatively large pixel size. The modulation transfer function of the camera with a P43 scintillator has been determined for 400 keV electrons and show an amplitude fall-off. In order to evaluate the structural resolution that the camera can deliver in imaging protein crystals, the ice-embedded catalase crystal was used as a test specimen.

Original languageEnglish (US)
Pages98-99
Number of pages2
StatePublished - Dec 1 1994
Externally publishedYes
EventProceedings of the 52nd Annual Meeting of the Microscopy Society of America - New Orleans, LA, USA
Duration: Jul 31 1994Aug 5 1994

Other

OtherProceedings of the 52nd Annual Meeting of the Microscopy Society of America
CityNew Orleans, LA, USA
Period7/31/948/5/94

ASJC Scopus subject areas

  • Engineering(all)

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    Chiu, W., Sherman, M., & Brink, J. (1994). Spot-scan imaging of ice-embedded catalase crystal on a slow-scan CCD camera in a 400-kV electron cryomicroscope. 98-99. Paper presented at Proceedings of the 52nd Annual Meeting of the Microscopy Society of America, New Orleans, LA, USA, .