Spot-scan imaging of ice-embedded catalase crystal on a slow-scan CCD camera in a 400-kV electron cryomicroscope

Wah Chiu, Michael Sherman, Jaap Brink

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In protein electron crystallography, both low dose electron diffraction patterns and images are needed to provide accurate amplitudes and phases respectively for a 3-dimensional reconstruction. A limitation of the slow scan CCD camera for recording electron images of protein crystals arises from the relatively large pixel size. The modulation transfer function of the camera with a P43 scintillator has been determined for 400 keV electrons and show an amplitude fall-off. In order to evaluate the structural resolution that the camera can deliver in imaging protein crystals, the ice-embedded catalase crystal was used as a test specimen.

Original languageEnglish (US)
Title of host publicationProceedings - Annual Meeting, Microscopy Society of America
EditorsG.W. Bailey, A.J. Garratt-Reed
Pages98-99
Number of pages2
StatePublished - 1994
Externally publishedYes
EventProceedings of the 52nd Annual Meeting of the Microscopy Society of America - New Orleans, LA, USA
Duration: Jul 31 1994Aug 5 1994

Other

OtherProceedings of the 52nd Annual Meeting of the Microscopy Society of America
CityNew Orleans, LA, USA
Period7/31/948/5/94

Fingerprint

CCD cameras
Ice
Proteins
Imaging techniques
Crystals
Electrons
Cameras
Crystallography
Optical transfer function
Electron diffraction
Phosphors
Diffraction patterns
Pixels

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Chiu, W., Sherman, M., & Brink, J. (1994). Spot-scan imaging of ice-embedded catalase crystal on a slow-scan CCD camera in a 400-kV electron cryomicroscope. In G. W. Bailey, & A. J. Garratt-Reed (Eds.), Proceedings - Annual Meeting, Microscopy Society of America (pp. 98-99)

Spot-scan imaging of ice-embedded catalase crystal on a slow-scan CCD camera in a 400-kV electron cryomicroscope. / Chiu, Wah; Sherman, Michael; Brink, Jaap.

Proceedings - Annual Meeting, Microscopy Society of America. ed. / G.W. Bailey; A.J. Garratt-Reed. 1994. p. 98-99.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Chiu, W, Sherman, M & Brink, J 1994, Spot-scan imaging of ice-embedded catalase crystal on a slow-scan CCD camera in a 400-kV electron cryomicroscope. in GW Bailey & AJ Garratt-Reed (eds), Proceedings - Annual Meeting, Microscopy Society of America. pp. 98-99, Proceedings of the 52nd Annual Meeting of the Microscopy Society of America, New Orleans, LA, USA, 7/31/94.
Chiu W, Sherman M, Brink J. Spot-scan imaging of ice-embedded catalase crystal on a slow-scan CCD camera in a 400-kV electron cryomicroscope. In Bailey GW, Garratt-Reed AJ, editors, Proceedings - Annual Meeting, Microscopy Society of America. 1994. p. 98-99
Chiu, Wah ; Sherman, Michael ; Brink, Jaap. / Spot-scan imaging of ice-embedded catalase crystal on a slow-scan CCD camera in a 400-kV electron cryomicroscope. Proceedings - Annual Meeting, Microscopy Society of America. editor / G.W. Bailey ; A.J. Garratt-Reed. 1994. pp. 98-99
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