TY - JOUR
T1 - Squalane-based emulsion vaccine delivery system
T2 - composition with murabutide activate Th1 response
AU - Kantipakala, Ravi
AU - Bonam, Srinivasa Reddy
AU - Vemireddy, Sravanthi
AU - Miryala, Sreekanth
AU - Halmuthur M, Sampath Kumar
N1 - Publisher Copyright:
© 2018, © 2018 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2019/3/16
Y1 - 2019/3/16
N2 - Emulsions play an important role in present-day subunit vaccine delivery. Squalane-based emulsion was formulated using surfactants viz., Pluronic F68, Span 85 along with Murabutide (MB) as immunepotentiator. Particle size and zetapotential of the final optimized emulsion was found to be 134 nm and −13 mV, respectively. The in vitro cellular uptake studies performed using fluorescein isothiocyanate (FITC)-labeled ovalbumin (OVA) clearly revealed the rapid uptake of antigen in the presence of emulsion. The in vivo subcutaneous studies involving measurement of OVA-specific IgG antibody titers, Th1/Th2 cytokines were performed and a marked up regulation in IL-2, IL-12 and IFN-γ cytokines indicate Th1 immune response. Results supported that the squalane-based delivery system enhanced the uptake of the antigen by immune cells and elicited humoral as well as cell-mediated immune response in mice. These results indicate the promising application of the new squalane based oil-in-water (O/W) emulsion as capable vaccine delivery system useful for vaccine development.
AB - Emulsions play an important role in present-day subunit vaccine delivery. Squalane-based emulsion was formulated using surfactants viz., Pluronic F68, Span 85 along with Murabutide (MB) as immunepotentiator. Particle size and zetapotential of the final optimized emulsion was found to be 134 nm and −13 mV, respectively. The in vitro cellular uptake studies performed using fluorescein isothiocyanate (FITC)-labeled ovalbumin (OVA) clearly revealed the rapid uptake of antigen in the presence of emulsion. The in vivo subcutaneous studies involving measurement of OVA-specific IgG antibody titers, Th1/Th2 cytokines were performed and a marked up regulation in IL-2, IL-12 and IFN-γ cytokines indicate Th1 immune response. Results supported that the squalane-based delivery system enhanced the uptake of the antigen by immune cells and elicited humoral as well as cell-mediated immune response in mice. These results indicate the promising application of the new squalane based oil-in-water (O/W) emulsion as capable vaccine delivery system useful for vaccine development.
KW - IgG
KW - Squalane
KW - Th1/Th2 cytokines
KW - antigen uptake
KW - vaccine delivery
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U2 - 10.1080/10837450.2018.1469150
DO - 10.1080/10837450.2018.1469150
M3 - Article
C2 - 29688119
AN - SCOPUS:85049555069
SN - 1083-7450
VL - 24
SP - 269
EP - 275
JO - Pharmaceutical Development and Technology
JF - Pharmaceutical Development and Technology
IS - 3
ER -