Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions

Ok Ho Shin, Jeong Seop Rhee, Jiong Tang, Shuzo Sugita, Christian Rosenmund, Thomas C. Südhof

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Sr2+ triggers neurotransmitter release similar to Ca2+, but less efficiently. We now show that in synaptotagmin 1 knockout mice, the fast component of both Ca2+- and Sr2+-induced release is selectively impaired, suggesting that both cations partly act by binding to synaptotagmin 1. Both the C2A and the C2B domain of synaptotagmin 1 bind Ca2+ in phospholipid complexes, but only the C2B domain forms Sr2+/phospholipid complexes; therefore, Sr2+ binding to the C2B domain is sufficient to trigger fast release, although with decreased efficacy. Ca2+ induces binding of the synaptotagmin C2 domains to SNARE proteins, whereas Sr2+ even at high concentrations does not. Thus, triggering of the fast component of release by Sr2+ as a Ca2+ agonist involves the formation of synaptotagmin/phospholipid complexes, but does not require stimulated SNARE binding.

Original languageEnglish
Pages (from-to)99-108
Number of pages10
JournalNeuron
Volume37
Issue number1
DOIs
StatePublished - Jan 9 2003
Externally publishedYes

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Synaptotagmin I
SNARE Proteins
Exocytosis
Synaptotagmins
Phospholipids
Binding Sites
Knockout Mice
Neurotransmitter Agents
Cations

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions. / Shin, Ok Ho; Rhee, Jeong Seop; Tang, Jiong; Sugita, Shuzo; Rosenmund, Christian; Südhof, Thomas C.

In: Neuron, Vol. 37, No. 1, 09.01.2003, p. 99-108.

Research output: Contribution to journalArticle

Shin, Ok Ho ; Rhee, Jeong Seop ; Tang, Jiong ; Sugita, Shuzo ; Rosenmund, Christian ; Südhof, Thomas C. / Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions. In: Neuron. 2003 ; Vol. 37, No. 1. pp. 99-108.
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