Sr2+ binding to the Ca2+ binding site of the synaptotagmin 1 C2B domain triggers fast exocytosis without stimulating SNARE interactions

Ok Ho Shin, Jeong Seop Rhee, Jiong Tang, Shuzo Sugita, Christian Rosenmund, Thomas C. Südhof

Research output: Contribution to journalArticlepeer-review

104 Scopus citations


Sr2+ triggers neurotransmitter release similar to Ca2+, but less efficiently. We now show that in synaptotagmin 1 knockout mice, the fast component of both Ca2+- and Sr2+-induced release is selectively impaired, suggesting that both cations partly act by binding to synaptotagmin 1. Both the C2A and the C2B domain of synaptotagmin 1 bind Ca2+ in phospholipid complexes, but only the C2B domain forms Sr2+/phospholipid complexes; therefore, Sr2+ binding to the C2B domain is sufficient to trigger fast release, although with decreased efficacy. Ca2+ induces binding of the synaptotagmin C2 domains to SNARE proteins, whereas Sr2+ even at high concentrations does not. Thus, triggering of the fast component of release by Sr2+ as a Ca2+ agonist involves the formation of synaptotagmin/phospholipid complexes, but does not require stimulated SNARE binding.

Original languageEnglish (US)
Pages (from-to)99-108
Number of pages10
Issue number1
StatePublished - Jan 9 2003

ASJC Scopus subject areas

  • Neuroscience(all)


Dive into the research topics of 'Sr<sup>2+</sup> binding to the Ca<sup>2+</sup> binding site of the synaptotagmin 1 C<sub>2</sub>B domain triggers fast exocytosis without stimulating SNARE interactions'. Together they form a unique fingerprint.

Cite this