Stability of RNA virus attenuation approaches

Joan L. Kenney, Sara M. Volk, Jyotsna Pandya, Eryu Wang, Xiaodong Liang, Scott C. Weaver

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


The greatest risk from live-attenuated vaccines is reversion to virulence. Particular concerns arise for RNA viruses, which exhibit high mutation frequencies. We examined the stability of 3 attenuation strategies for the alphavirus, Venezuelan equine encephalitis virus (VEEV): a traditional, point mutation-dependent attenuation approach exemplified by TC-83; a rationally designed, targeted-mutation approach represented by V3526; and a chimeric vaccine, SIN/TC/ZPC. Our findings suggest that the chimeric strain combines the initial attenuation of TC-83 with the greater phenotypic stability of V3526, highlighting the importance of the both initial attenuation and stability for live-attenuated vaccines.

Original languageEnglish (US)
Pages (from-to)2230-2234
Number of pages5
Issue number12
StatePublished - 2011


  • Alphavirus
  • RNA viruses
  • Vaccine stability
  • Venezuelan equine encephalitis virus

ASJC Scopus subject areas

  • Molecular Medicine
  • General Immunology and Microbiology
  • General Veterinary
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases


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