Stability of RNA virus attenuation approaches

Joan L. Kenney, Sara M. Volk, Jyotsna Pandya, Eryu Wang, Xiaodong Liang, Scott Weaver

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The greatest risk from live-attenuated vaccines is reversion to virulence. Particular concerns arise for RNA viruses, which exhibit high mutation frequencies. We examined the stability of 3 attenuation strategies for the alphavirus, Venezuelan equine encephalitis virus (VEEV): a traditional, point mutation-dependent attenuation approach exemplified by TC-83; a rationally designed, targeted-mutation approach represented by V3526; and a chimeric vaccine, SIN/TC/ZPC. Our findings suggest that the chimeric strain combines the initial attenuation of TC-83 with the greater phenotypic stability of V3526, highlighting the importance of the both initial attenuation and stability for live-attenuated vaccines.

Original languageEnglish (US)
Pages (from-to)2230-2234
Number of pages5
JournalVaccine
Volume29
Issue number12
DOIs
StatePublished - 2011

Fingerprint

Attenuated Vaccines
RNA Viruses
live vaccines
Venezuelan Equine Encephalitis Viruses
Alphavirus
Mutation Rate
Point Mutation
Virulence
Vaccines
Venezuelan equine encephalitis virus
mutation
Mutation
point mutation
virulence
vaccines
RNA viruses

Keywords

  • Alphavirus
  • RNA viruses
  • Vaccine stability
  • Venezuelan equine encephalitis virus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

Cite this

Kenney, J. L., Volk, S. M., Pandya, J., Wang, E., Liang, X., & Weaver, S. (2011). Stability of RNA virus attenuation approaches. Vaccine, 29(12), 2230-2234. https://doi.org/10.1016/j.vaccine.2011.01.055

Stability of RNA virus attenuation approaches. / Kenney, Joan L.; Volk, Sara M.; Pandya, Jyotsna; Wang, Eryu; Liang, Xiaodong; Weaver, Scott.

In: Vaccine, Vol. 29, No. 12, 2011, p. 2230-2234.

Research output: Contribution to journalArticle

Kenney, JL, Volk, SM, Pandya, J, Wang, E, Liang, X & Weaver, S 2011, 'Stability of RNA virus attenuation approaches', Vaccine, vol. 29, no. 12, pp. 2230-2234. https://doi.org/10.1016/j.vaccine.2011.01.055
Kenney JL, Volk SM, Pandya J, Wang E, Liang X, Weaver S. Stability of RNA virus attenuation approaches. Vaccine. 2011;29(12):2230-2234. https://doi.org/10.1016/j.vaccine.2011.01.055
Kenney, Joan L. ; Volk, Sara M. ; Pandya, Jyotsna ; Wang, Eryu ; Liang, Xiaodong ; Weaver, Scott. / Stability of RNA virus attenuation approaches. In: Vaccine. 2011 ; Vol. 29, No. 12. pp. 2230-2234.
@article{c7b4a174a5594b41adddcdbd14cebeb3,
title = "Stability of RNA virus attenuation approaches",
abstract = "The greatest risk from live-attenuated vaccines is reversion to virulence. Particular concerns arise for RNA viruses, which exhibit high mutation frequencies. We examined the stability of 3 attenuation strategies for the alphavirus, Venezuelan equine encephalitis virus (VEEV): a traditional, point mutation-dependent attenuation approach exemplified by TC-83; a rationally designed, targeted-mutation approach represented by V3526; and a chimeric vaccine, SIN/TC/ZPC. Our findings suggest that the chimeric strain combines the initial attenuation of TC-83 with the greater phenotypic stability of V3526, highlighting the importance of the both initial attenuation and stability for live-attenuated vaccines.",
keywords = "Alphavirus, RNA viruses, Vaccine stability, Venezuelan equine encephalitis virus",
author = "Kenney, {Joan L.} and Volk, {Sara M.} and Jyotsna Pandya and Eryu Wang and Xiaodong Liang and Scott Weaver",
year = "2011",
doi = "10.1016/j.vaccine.2011.01.055",
language = "English (US)",
volume = "29",
pages = "2230--2234",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "12",

}

TY - JOUR

T1 - Stability of RNA virus attenuation approaches

AU - Kenney, Joan L.

AU - Volk, Sara M.

AU - Pandya, Jyotsna

AU - Wang, Eryu

AU - Liang, Xiaodong

AU - Weaver, Scott

PY - 2011

Y1 - 2011

N2 - The greatest risk from live-attenuated vaccines is reversion to virulence. Particular concerns arise for RNA viruses, which exhibit high mutation frequencies. We examined the stability of 3 attenuation strategies for the alphavirus, Venezuelan equine encephalitis virus (VEEV): a traditional, point mutation-dependent attenuation approach exemplified by TC-83; a rationally designed, targeted-mutation approach represented by V3526; and a chimeric vaccine, SIN/TC/ZPC. Our findings suggest that the chimeric strain combines the initial attenuation of TC-83 with the greater phenotypic stability of V3526, highlighting the importance of the both initial attenuation and stability for live-attenuated vaccines.

AB - The greatest risk from live-attenuated vaccines is reversion to virulence. Particular concerns arise for RNA viruses, which exhibit high mutation frequencies. We examined the stability of 3 attenuation strategies for the alphavirus, Venezuelan equine encephalitis virus (VEEV): a traditional, point mutation-dependent attenuation approach exemplified by TC-83; a rationally designed, targeted-mutation approach represented by V3526; and a chimeric vaccine, SIN/TC/ZPC. Our findings suggest that the chimeric strain combines the initial attenuation of TC-83 with the greater phenotypic stability of V3526, highlighting the importance of the both initial attenuation and stability for live-attenuated vaccines.

KW - Alphavirus

KW - RNA viruses

KW - Vaccine stability

KW - Venezuelan equine encephalitis virus

UR - http://www.scopus.com/inward/record.url?scp=79951809232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79951809232&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2011.01.055

DO - 10.1016/j.vaccine.2011.01.055

M3 - Article

VL - 29

SP - 2230

EP - 2234

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 12

ER -