Standardization of Bovine Macrophage Monolayers and Isolation and Culture of Trypanosomes

Omar A. Saldarriaga, Jaime I. Velásquez, Jorge E. Ossa, Maria T. Rugeles

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.

Original languageEnglish (US)
Pages (from-to)269-271
Number of pages3
JournalMemorias do Instituto Oswaldo Cruz
Volume98
Issue number2
StatePublished - Mar 2003
Externally publishedYes

Fingerprint

Trypanosomiasis
Macrophages
Cell culture
Standardization
Monolayers
Esterases
Trypanosoma
Blood
Cultured Cells
Blood Cells
Cell Count
Research

Keywords

  • Bovine
  • Macrophages
  • Trypanosomes

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology
  • Immunology
  • Microbiology (medical)
  • Clinical Biochemistry

Cite this

Saldarriaga, O. A., Velásquez, J. I., Ossa, J. E., & Rugeles, M. T. (2003). Standardization of Bovine Macrophage Monolayers and Isolation and Culture of Trypanosomes. Memorias do Instituto Oswaldo Cruz, 98(2), 269-271.

Standardization of Bovine Macrophage Monolayers and Isolation and Culture of Trypanosomes. / Saldarriaga, Omar A.; Velásquez, Jaime I.; Ossa, Jorge E.; Rugeles, Maria T.

In: Memorias do Instituto Oswaldo Cruz, Vol. 98, No. 2, 03.2003, p. 269-271.

Research output: Contribution to journalArticle

Saldarriaga, OA, Velásquez, JI, Ossa, JE & Rugeles, MT 2003, 'Standardization of Bovine Macrophage Monolayers and Isolation and Culture of Trypanosomes', Memorias do Instituto Oswaldo Cruz, vol. 98, no. 2, pp. 269-271.
Saldarriaga, Omar A. ; Velásquez, Jaime I. ; Ossa, Jorge E. ; Rugeles, Maria T. / Standardization of Bovine Macrophage Monolayers and Isolation and Culture of Trypanosomes. In: Memorias do Instituto Oswaldo Cruz. 2003 ; Vol. 98, No. 2. pp. 269-271.
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