Standardization of Bovine Macrophage Monolayers and Isolation and Culture of Trypanosomes

Omar A. Saldarriaga, Jaime I. Velásquez, Jorge E. Ossa, Maria T. Rugeles

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We describe a method for culturing over 90% pure bovine macrophages from peripheral blood mononuclear cells separated with Nycoprep. The cells were cultured for 12 days and then stained with esterase and with anti CD14 to test for purity. The method is reproducible and ensures an adequate number of cells for immunological research. Additionally, we report the unexpected finding of Trypanosoma trypomastigotes in our macrophage cultures from bovines belonging to a geographic area from which no bovine trypanosomes had been reported before.

Original languageEnglish (US)
Pages (from-to)269-271
Number of pages3
JournalMemorias do Instituto Oswaldo Cruz
Volume98
Issue number2
DOIs
StatePublished - Mar 2003

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Keywords

  • Bovine
  • Macrophages
  • Trypanosomes

ASJC Scopus subject areas

  • Microbiology (medical)

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