Standardization of Quantitative Plaque-Based Viral Assays for Orthoflavivirus Cacipacoré

Cacipacoré virus (CPCV) is an understudied orthoflavivirus with significant gaps in research that hinders our understanding of its ecology, host range, and potential public health impact. A notable barrier to filling this gap is the absence of standardized methodologies for viral quantification, such as plaque-forming and focus-forming assays. This short communication outlines the development of optimized protocols for CPCV titration in two cell lines, Vero CCL-81 and BHK CCL-10, using several overlay and time point conditions. These methodologies address the need for robust quantitative tools to advance research on CPCV and its implications for human and animal health, fostering progress in the surveillance and understanding of this underexplored arbovirus.