Starvation-iInduced proximal gut mucosal atrophy diminished with aging

Juquan Song, Steven Wolf, Xiao Wu Wu, Celeste Finnerty, Gerd G. Gauglitz, David Herndon, Marc G. Jeschke

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background: Starvation induces small bowel atrophy with increased intestinal epithelial apoptosis and decreased proliferation. The authors examined these parameters after starvation in aged animals. Methods: Sixty-four 6-week-old and 26-month-old C57BL/6 mice were randomly assigned to either an ad libitum fed or fasted group. The small bowel was harvested at 12, 48, and 72 hours following starvation. Proximal gut mucosal height was measured and epithelial cells counted. Apoptosis was identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Proliferation was determined by immunohistochemical staining for proliferating cell nuclear antigen. Comparison of fed vs fasted and adult vs old groups was done by one-way ANOVA with Tukey's test and unpaired Student's t test. Significance was accepted at P <.05. Results: Aged mice had higher proximal gut weights, mucosal heights, and cell numbers at baseline compared with the adult group (P <.05). The rate of apoptosis was lower in the aged (P <.05), but proliferation was not different between groups before starvation. After starvation, proximal gut wet weight decreased only in adult mice (P <.05). Gut mucosal height and mucosal cell number decreased more in adult than in aged mice (P <.05). This was related to decreased proliferation only in the adult group (P <.05). The fold of epithelial apoptosis that increased was higher in the aged group than in the adult group after starvation (P <.05). Conclusions: Gut mucosal kinetics change with age and have lower rates of apoptosis and greater mucosal mass; the character of starvation-induced atrophy is diminished with aging.

Original languageEnglish (US)
Pages (from-to)411-416
Number of pages6
JournalJournal of Parenteral and Enteral Nutrition
Volume33
Issue number4
DOIs
StatePublished - Jul 2009

Fingerprint

Starvation
Atrophy
Apoptosis
Cell Count
Staining and Labeling
Weights and Measures
DNA Nucleotidylexotransferase
Proliferating Cell Nuclear Antigen
Inbred C57BL Mouse
Analysis of Variance
Epithelial Cells
Students

Keywords

  • Apoptosis
  • Fasting
  • Mucosal homeostasis
  • PCNA staining
  • Proliferation
  • Senescent
  • TUNEL staining

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Starvation-iInduced proximal gut mucosal atrophy diminished with aging. / Song, Juquan; Wolf, Steven; Wu, Xiao Wu; Finnerty, Celeste; Gauglitz, Gerd G.; Herndon, David; Jeschke, Marc G.

In: Journal of Parenteral and Enteral Nutrition, Vol. 33, No. 4, 07.2009, p. 411-416.

Research output: Contribution to journalArticle

Song, Juquan ; Wolf, Steven ; Wu, Xiao Wu ; Finnerty, Celeste ; Gauglitz, Gerd G. ; Herndon, David ; Jeschke, Marc G. / Starvation-iInduced proximal gut mucosal atrophy diminished with aging. In: Journal of Parenteral and Enteral Nutrition. 2009 ; Vol. 33, No. 4. pp. 411-416.
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abstract = "Background: Starvation induces small bowel atrophy with increased intestinal epithelial apoptosis and decreased proliferation. The authors examined these parameters after starvation in aged animals. Methods: Sixty-four 6-week-old and 26-month-old C57BL/6 mice were randomly assigned to either an ad libitum fed or fasted group. The small bowel was harvested at 12, 48, and 72 hours following starvation. Proximal gut mucosal height was measured and epithelial cells counted. Apoptosis was identified by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining. Proliferation was determined by immunohistochemical staining for proliferating cell nuclear antigen. Comparison of fed vs fasted and adult vs old groups was done by one-way ANOVA with Tukey's test and unpaired Student's t test. Significance was accepted at P <.05. Results: Aged mice had higher proximal gut weights, mucosal heights, and cell numbers at baseline compared with the adult group (P <.05). The rate of apoptosis was lower in the aged (P <.05), but proliferation was not different between groups before starvation. After starvation, proximal gut wet weight decreased only in adult mice (P <.05). Gut mucosal height and mucosal cell number decreased more in adult than in aged mice (P <.05). This was related to decreased proliferation only in the adult group (P <.05). The fold of epithelial apoptosis that increased was higher in the aged group than in the adult group after starvation (P <.05). Conclusions: Gut mucosal kinetics change with age and have lower rates of apoptosis and greater mucosal mass; the character of starvation-induced atrophy is diminished with aging.",
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