STAT3 deletion during hematopoiesis causes Crohn's disease-like pathogenesis and lethality

A critical role of STAT3 in innate immunity

Thomas Welte, Samuel S M Zhang, Tian Wang, Zhiyuan Zhang, David G T Hesslein, Zhinan Yin, Arihiro Kano, Yoshiki Iwamoto, En Li, Joseph E. Craft, Alfred L M Bothwell, Erol Fikrig, Pandelakis A. Koni, Richard A. Flavell, Xin Yuan Fu

Research output: Contribution to journalArticle

299 Citations (Scopus)

Abstract

Signal transducer and activator of transcription 3 (STAT3) is a key transcriptional mediator for many cytokines and is essential for normal embryonic development. We have generated a unique strain of mice with tissue-specific disruption of STAT3 in bone marrow cells during hematopoiesis. This specific STAT3 deletion causes death of these mice within 4-6 weeks after birth with Crohn's disease-like pathogenesis in both the small and large intestine, including segmental inflammatory cell infiltration, ulceration, bowel wall thickening, and granuloma formation. Deletion of STAT3 causes significantly increased cell autonomous proliferation of cells of the myeloid lineage, both in vivo and in vitro. Most importantly, Stat3 deletion during hematopoiesis causes overly pseudoactivated innate immune responses. Although inflammatory cytokines, including tumor necrosis factor α and IFN-γ, are overly produced in these mice, the NAPDH oxidase activity, which is involved in antimicrobial and innate immune responses, is inhibited. The signaling responses to lipopolysaccharide are changed in the absence of STAT3, leading to enhanced NF-κB activation. Our results suggest a model in which STAT3 has critical roles in the development and regulation of innate immunity, and deletion of STAT3 during hematopoiesis results in abnormalities in myeloid cells and causes Crohn's disease-like pathogenesis.

Original languageEnglish (US)
Pages (from-to)1879-1884
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number4
DOIs
StatePublished - Feb 18 2003
Externally publishedYes

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STAT3 Transcription Factor
Hematopoiesis
Innate Immunity
Crohn Disease
Cytokines
Large Intestine
Cell Lineage
Myeloid Cells
Granuloma
Bone Marrow Cells
Small Intestine
Embryonic Development
Lipopolysaccharides
Cause of Death
Oxidoreductases
Tumor Necrosis Factor-alpha
Cell Proliferation
Parturition

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

STAT3 deletion during hematopoiesis causes Crohn's disease-like pathogenesis and lethality : A critical role of STAT3 in innate immunity. / Welte, Thomas; Zhang, Samuel S M; Wang, Tian; Zhang, Zhiyuan; Hesslein, David G T; Yin, Zhinan; Kano, Arihiro; Iwamoto, Yoshiki; Li, En; Craft, Joseph E.; Bothwell, Alfred L M; Fikrig, Erol; Koni, Pandelakis A.; Flavell, Richard A.; Fu, Xin Yuan.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 4, 18.02.2003, p. 1879-1884.

Research output: Contribution to journalArticle

Welte, T, Zhang, SSM, Wang, T, Zhang, Z, Hesslein, DGT, Yin, Z, Kano, A, Iwamoto, Y, Li, E, Craft, JE, Bothwell, ALM, Fikrig, E, Koni, PA, Flavell, RA & Fu, XY 2003, 'STAT3 deletion during hematopoiesis causes Crohn's disease-like pathogenesis and lethality: A critical role of STAT3 in innate immunity', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 4, pp. 1879-1884. https://doi.org/10.1073/pnas.0237137100
Welte, Thomas ; Zhang, Samuel S M ; Wang, Tian ; Zhang, Zhiyuan ; Hesslein, David G T ; Yin, Zhinan ; Kano, Arihiro ; Iwamoto, Yoshiki ; Li, En ; Craft, Joseph E. ; Bothwell, Alfred L M ; Fikrig, Erol ; Koni, Pandelakis A. ; Flavell, Richard A. ; Fu, Xin Yuan. / STAT3 deletion during hematopoiesis causes Crohn's disease-like pathogenesis and lethality : A critical role of STAT3 in innate immunity. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 4. pp. 1879-1884.
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