Stearic Acid Stimulates FA Ethyl Ester Synthesis in HepG2 Cells Exposed to Ethanol

Ali Hasaba, Joanne E. Cluette-Brown, Michael Laposata

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


FA ethyl esters (FAEE) are nonoxidative metabolites of ethanol produced by the esterification of FA and ethanol. FAEE have been implicated as mediators of ethanol-induced organ damage in vivo and in vitro, and are markers of ethanol intake. Upon ethanol intake, FAEE are synthesized in the liver and pancreas in significant quantities. There is limited information on the stimulation of FAEE synthesis upon addition of exogenous FA in vitro. HepG2 cells were incubated with ethanol alone, ethanol with 25 pM linoleate, and ethanol with 25 pM stearate. The amount of FAEE in human hepatoblastoma (HepG2) cells was determined 1-3 h after ethanol and FA addition. Stearate increased the FAEE concentration in HepG2 cells when incubated with the cells for 1 h, whereas linoleate did not increase the cellular FAEE concentration at any time. Ethyl palmitate, ethyl stearate, and ethyl oleate were the predominant FAEE species identified in all cases, independent of the specific supplemental FA added to the medium.

Original languageEnglish (US)
Pages (from-to)1051-1055
Number of pages5
Issue number10
StatePublished - Oct 2003
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Cell Biology


Dive into the research topics of 'Stearic Acid Stimulates FA Ethyl Ester Synthesis in HepG2 Cells Exposed to Ethanol'. Together they form a unique fingerprint.

Cite this