Stearic Acid Stimulates FA Ethyl Ester Synthesis in HepG2 Cells Exposed to Ethanol

Ali Hasaba, Joanne E. Cluette-Brown, Michael Laposata

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

FA ethyl esters (FAEE) are nonoxidative metabolites of ethanol produced by the esterification of FA and ethanol. FAEE have been implicated as mediators of ethanol-induced organ damage in vivo and in vitro, and are markers of ethanol intake. Upon ethanol intake, FAEE are synthesized in the liver and pancreas in significant quantities. There is limited information on the stimulation of FAEE synthesis upon addition of exogenous FA in vitro. HepG2 cells were incubated with ethanol alone, ethanol with 25 pM linoleate, and ethanol with 25 pM stearate. The amount of FAEE in human hepatoblastoma (HepG2) cells was determined 1-3 h after ethanol and FA addition. Stearate increased the FAEE concentration in HepG2 cells when incubated with the cells for 1 h, whereas linoleate did not increase the cellular FAEE concentration at any time. Ethyl palmitate, ethyl stearate, and ethyl oleate were the predominant FAEE species identified in all cases, independent of the specific supplemental FA added to the medium.

Original languageEnglish (US)
Pages (from-to)1051-1055
Number of pages5
JournalLipids
Volume38
Issue number10
StatePublished - Oct 2003
Externally publishedYes

Fingerprint

Hep G2 Cells
stearic acid
Esters
Ethanol
esters
ethanol
synthesis
cells
Stearates
Linoleic Acid
Hepatoblastoma
Esterification
palmitates
esterification
Metabolites
pancreas
Liver
oleic acid
Pancreas
metabolites

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Biochemistry

Cite this

Stearic Acid Stimulates FA Ethyl Ester Synthesis in HepG2 Cells Exposed to Ethanol. / Hasaba, Ali; Cluette-Brown, Joanne E.; Laposata, Michael.

In: Lipids, Vol. 38, No. 10, 10.2003, p. 1051-1055.

Research output: Contribution to journalArticle

Hasaba, A, Cluette-Brown, JE & Laposata, M 2003, 'Stearic Acid Stimulates FA Ethyl Ester Synthesis in HepG2 Cells Exposed to Ethanol', Lipids, vol. 38, no. 10, pp. 1051-1055.
Hasaba, Ali ; Cluette-Brown, Joanne E. ; Laposata, Michael. / Stearic Acid Stimulates FA Ethyl Ester Synthesis in HepG2 Cells Exposed to Ethanol. In: Lipids. 2003 ; Vol. 38, No. 10. pp. 1051-1055.
@article{4647d3a329e048ffa4a052eedf5b1f29,
title = "Stearic Acid Stimulates FA Ethyl Ester Synthesis in HepG2 Cells Exposed to Ethanol",
abstract = "FA ethyl esters (FAEE) are nonoxidative metabolites of ethanol produced by the esterification of FA and ethanol. FAEE have been implicated as mediators of ethanol-induced organ damage in vivo and in vitro, and are markers of ethanol intake. Upon ethanol intake, FAEE are synthesized in the liver and pancreas in significant quantities. There is limited information on the stimulation of FAEE synthesis upon addition of exogenous FA in vitro. HepG2 cells were incubated with ethanol alone, ethanol with 25 pM linoleate, and ethanol with 25 pM stearate. The amount of FAEE in human hepatoblastoma (HepG2) cells was determined 1-3 h after ethanol and FA addition. Stearate increased the FAEE concentration in HepG2 cells when incubated with the cells for 1 h, whereas linoleate did not increase the cellular FAEE concentration at any time. Ethyl palmitate, ethyl stearate, and ethyl oleate were the predominant FAEE species identified in all cases, independent of the specific supplemental FA added to the medium.",
author = "Ali Hasaba and Cluette-Brown, {Joanne E.} and Michael Laposata",
year = "2003",
month = "10",
language = "English (US)",
volume = "38",
pages = "1051--1055",
journal = "Lipids",
issn = "0024-4201",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - Stearic Acid Stimulates FA Ethyl Ester Synthesis in HepG2 Cells Exposed to Ethanol

AU - Hasaba, Ali

AU - Cluette-Brown, Joanne E.

AU - Laposata, Michael

PY - 2003/10

Y1 - 2003/10

N2 - FA ethyl esters (FAEE) are nonoxidative metabolites of ethanol produced by the esterification of FA and ethanol. FAEE have been implicated as mediators of ethanol-induced organ damage in vivo and in vitro, and are markers of ethanol intake. Upon ethanol intake, FAEE are synthesized in the liver and pancreas in significant quantities. There is limited information on the stimulation of FAEE synthesis upon addition of exogenous FA in vitro. HepG2 cells were incubated with ethanol alone, ethanol with 25 pM linoleate, and ethanol with 25 pM stearate. The amount of FAEE in human hepatoblastoma (HepG2) cells was determined 1-3 h after ethanol and FA addition. Stearate increased the FAEE concentration in HepG2 cells when incubated with the cells for 1 h, whereas linoleate did not increase the cellular FAEE concentration at any time. Ethyl palmitate, ethyl stearate, and ethyl oleate were the predominant FAEE species identified in all cases, independent of the specific supplemental FA added to the medium.

AB - FA ethyl esters (FAEE) are nonoxidative metabolites of ethanol produced by the esterification of FA and ethanol. FAEE have been implicated as mediators of ethanol-induced organ damage in vivo and in vitro, and are markers of ethanol intake. Upon ethanol intake, FAEE are synthesized in the liver and pancreas in significant quantities. There is limited information on the stimulation of FAEE synthesis upon addition of exogenous FA in vitro. HepG2 cells were incubated with ethanol alone, ethanol with 25 pM linoleate, and ethanol with 25 pM stearate. The amount of FAEE in human hepatoblastoma (HepG2) cells was determined 1-3 h after ethanol and FA addition. Stearate increased the FAEE concentration in HepG2 cells when incubated with the cells for 1 h, whereas linoleate did not increase the cellular FAEE concentration at any time. Ethyl palmitate, ethyl stearate, and ethyl oleate were the predominant FAEE species identified in all cases, independent of the specific supplemental FA added to the medium.

UR - http://www.scopus.com/inward/record.url?scp=0344392971&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344392971&partnerID=8YFLogxK

M3 - Article

C2 - 14669970

AN - SCOPUS:0344392971

VL - 38

SP - 1051

EP - 1055

JO - Lipids

JF - Lipids

SN - 0024-4201

IS - 10

ER -