Stem cell factor and IL-2 act synergistically in inducing intraepithelial lymphocyte proliferation and cytokine production

Upregulation of the IL-2 receptor γ-chain and signaling via JAK-3

Tian Wang, R. Alam, K. E. Langley, G. R. Klimpel

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Murine intraepithelial lymphocytes (IEL) that express the gamma/delta form of the T cell receptor for antigen (TCRγδ) also express c-kit, the receptor for stem cell factor (SCF). We show here that SCF upregulates the expression of γδ TCR on IEL. More importantly, SCF induces upregulation in the expression of the common γ-chain (γc), which is a shared subunit of the receptor complexes for IL-2, -4, -7, -9, and -15. SCF was shown to act synergistically with IL-2 in inducing IEL proliferation, IFNγ production, non-MHC-restricted cytotoxic activity, and upregulation of the expression of the γc. SCF also acted synergistically with IL-7 and IL-15 in inducing IEL proliferation. IEL exposed to SCF were shown to have enhanced phosphorylation of JAK-3, and when SCF was combined with IL-2, there was an enhancement in the phosphorylation of JAK-3. These results suggest that SCF may play a more important role in regulating mucosal immune responses than previously appreciated. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)62-71
Number of pages10
JournalCellular Immunology
Volume205
Issue number1
DOIs
StatePublished - Oct 10 2000

Fingerprint

Stem Cell Factor
Interleukin-2 Receptors
Interleukin-2
Up-Regulation
Lymphocytes
Cytokines
Antigen Receptors, T-Cell, gamma-delta
Phosphorylation
Proto-Oncogene Proteins c-kit
Interleukin-15
Interleukin-7
Mucosal Immunity
T-Cell Antigen Receptor
Interleukin-4

Keywords

  • Cytokines
  • IEL
  • Stem cell factor

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

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title = "Stem cell factor and IL-2 act synergistically in inducing intraepithelial lymphocyte proliferation and cytokine production: Upregulation of the IL-2 receptor γ-chain and signaling via JAK-3",
abstract = "Murine intraepithelial lymphocytes (IEL) that express the gamma/delta form of the T cell receptor for antigen (TCRγδ) also express c-kit, the receptor for stem cell factor (SCF). We show here that SCF upregulates the expression of γδ TCR on IEL. More importantly, SCF induces upregulation in the expression of the common γ-chain (γc), which is a shared subunit of the receptor complexes for IL-2, -4, -7, -9, and -15. SCF was shown to act synergistically with IL-2 in inducing IEL proliferation, IFNγ production, non-MHC-restricted cytotoxic activity, and upregulation of the expression of the γc. SCF also acted synergistically with IL-7 and IL-15 in inducing IEL proliferation. IEL exposed to SCF were shown to have enhanced phosphorylation of JAK-3, and when SCF was combined with IL-2, there was an enhancement in the phosphorylation of JAK-3. These results suggest that SCF may play a more important role in regulating mucosal immune responses than previously appreciated. (C) 2000 Academic Press.",
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T2 - Upregulation of the IL-2 receptor γ-chain and signaling via JAK-3

AU - Wang, Tian

AU - Alam, R.

AU - Langley, K. E.

AU - Klimpel, G. R.

PY - 2000/10/10

Y1 - 2000/10/10

N2 - Murine intraepithelial lymphocytes (IEL) that express the gamma/delta form of the T cell receptor for antigen (TCRγδ) also express c-kit, the receptor for stem cell factor (SCF). We show here that SCF upregulates the expression of γδ TCR on IEL. More importantly, SCF induces upregulation in the expression of the common γ-chain (γc), which is a shared subunit of the receptor complexes for IL-2, -4, -7, -9, and -15. SCF was shown to act synergistically with IL-2 in inducing IEL proliferation, IFNγ production, non-MHC-restricted cytotoxic activity, and upregulation of the expression of the γc. SCF also acted synergistically with IL-7 and IL-15 in inducing IEL proliferation. IEL exposed to SCF were shown to have enhanced phosphorylation of JAK-3, and when SCF was combined with IL-2, there was an enhancement in the phosphorylation of JAK-3. These results suggest that SCF may play a more important role in regulating mucosal immune responses than previously appreciated. (C) 2000 Academic Press.

AB - Murine intraepithelial lymphocytes (IEL) that express the gamma/delta form of the T cell receptor for antigen (TCRγδ) also express c-kit, the receptor for stem cell factor (SCF). We show here that SCF upregulates the expression of γδ TCR on IEL. More importantly, SCF induces upregulation in the expression of the common γ-chain (γc), which is a shared subunit of the receptor complexes for IL-2, -4, -7, -9, and -15. SCF was shown to act synergistically with IL-2 in inducing IEL proliferation, IFNγ production, non-MHC-restricted cytotoxic activity, and upregulation of the expression of the γc. SCF also acted synergistically with IL-7 and IL-15 in inducing IEL proliferation. IEL exposed to SCF were shown to have enhanced phosphorylation of JAK-3, and when SCF was combined with IL-2, there was an enhancement in the phosphorylation of JAK-3. These results suggest that SCF may play a more important role in regulating mucosal immune responses than previously appreciated. (C) 2000 Academic Press.

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