Stem cell factor (SCF) can regulate the activation and expansion of murine intraepithelial lymphocytes

Tian Wang, Keith E. Langley, William K. Gourley, Gary R. Klimpel

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Murine intraepithelial lymphocytes (IEL) express c-kit, the receptor for stem cell factor (SCF). SCF induced a low but significant proliferative response in IEL, but not in splenic T cells. SCF stimulation of IEL resulted in an expansion of the c-kit+, TCRγδ+ cell population. SCF-induced proliferation was dependent upon SCF-c-kit interactions, since antibody to c-kit blocked this response, and TEL obtained from c-kit mutant (W/W(v)) mice failed to respond to SCF. SCF acted synergistically with anti-TCRγδ and with concavalin A (Con A) to induce proliferation and interferon γ (IFN-γ) production in IEL. Finally, mice injected with SCF had a significant increase in the number of IEL in the small intestine. SCF-treated mice had increased numbers of TCRαβ+ and TCRγδ+ cell populations, as well as increased numbers of c-kit+ and c-kit- IEL. These data suggest that SCF-c-kit interactions play an important role in regulating IEL expansion and activation. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)272-280
Number of pages9
JournalCytokine
Volume12
Issue number3
DOIs
StatePublished - Mar 2000

Fingerprint

Stem Cell Factor
Lymphocytes
Chemical activation
Lymphocyte Activation
Cells
Proto-Oncogene Proteins c-kit
T-cells
Lymphocyte Count
Interferons
Population
Small Intestine
T-Lymphocytes

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Stem cell factor (SCF) can regulate the activation and expansion of murine intraepithelial lymphocytes. / Wang, Tian; Langley, Keith E.; Gourley, William K.; Klimpel, Gary R.

In: Cytokine, Vol. 12, No. 3, 03.2000, p. 272-280.

Research output: Contribution to journalArticle

Wang, Tian ; Langley, Keith E. ; Gourley, William K. ; Klimpel, Gary R. / Stem cell factor (SCF) can regulate the activation and expansion of murine intraepithelial lymphocytes. In: Cytokine. 2000 ; Vol. 12, No. 3. pp. 272-280.
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