Sterile α motif domain containing 9 is a novel cellular interacting partner to low-risk type human papillomavirus E6 proteins

Jia Wang, Crystal Dupuis, Stephen K. Tyring, Michael Underbrink

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Low-risk type human papillomavirus (HPV) 6 and 11 infection causes recurrent respiratory papillomatosis (RRP) and genital warts. RRP is the most common benign tumor of the larynx in children with frequent relapses. Repeated surgeries are often needed to improve vocal function and prevent life-threatening respiratory obstruction. Currently, there are no effective treatments available to completely eliminate these diseases, largely due to limited knowledge regarding their viral molecular pathogenesis. HPV E6 proteins contribute to cell immortalization by interacting with a variety of cellular proteins, which have been well studied for the high-risk type HPVs related to cancer progression. However, the functions of low-risk HPV E6 proteins are largely unknown. In this study, we report GST-pulldown coupled mass spectrometry analysis with low-risk HPV E6 proteins that identified sterile alpha motif domain containing 9 (SAMD9) as a novel interacting partner. We then confirmed the interaction between HPV-E6 and SAMD9 using co-immunoprecipitation, proximity ligation assay, and confocal immunofluorescence staining. The SAMD9 gene is down-regulated in a variety of neoplasms and deleteriously mutated in normophosphatemic familial tumoral calcinosis. Interestingly, SAMD9 also has antiviral functions against poxvirus. Our study adds to the limited knowledge of the molecular properties of low-risk HPVs and describes new potential functions for the low-risk HPV E6 protein.

Original languageEnglish (US)
Article numbere0149859
JournalPLoS One
Volume11
Issue number2
DOIs
StatePublished - Feb 1 2016

Fingerprint

Papillomaviridae
Human papillomavirus 6
Proteins
papilloma
proteins
neoplasms
Poxviridae
calcinosis
Human papillomavirus 11
Condylomata Acuminata
warts
Neoplasms
larynx
relapse
Larynx
Immunoprecipitation
Surgery
fluorescent antibody technique
genitalia
Antiviral Agents

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sterile α motif domain containing 9 is a novel cellular interacting partner to low-risk type human papillomavirus E6 proteins. / Wang, Jia; Dupuis, Crystal; Tyring, Stephen K.; Underbrink, Michael.

In: PLoS One, Vol. 11, No. 2, e0149859, 01.02.2016.

Research output: Contribution to journalArticle

Wang, Jia ; Dupuis, Crystal ; Tyring, Stephen K. ; Underbrink, Michael. / Sterile α motif domain containing 9 is a novel cellular interacting partner to low-risk type human papillomavirus E6 proteins. In: PLoS One. 2016 ; Vol. 11, No. 2.
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