Steroid induction of nerve growth factor synthesis in cell culture

J. R. Perez-Polo, K. Hall, K. Livingston, K. Westlund

    Research output: Contribution to journalArticle

    72 Citations (Scopus)

    Abstract

    Nerve growth factor (NGF) is a peptide hormone which is necessary for the development of sympathetic neurons. Exposing a rat central nervous system glioma cell line (C-6) to the steroid hormone 17β-estradiol increases the amount of NGF secreted by these cells into the surrounding medium. This induction is highly specific to 17β-estradiol in that similar steroids do not increase NGF levels. Both NGF activity and protein levels increase upon estradiol stimulation and there is a parallel increase in NGF de novo synthesis. The estradiol effect can be blocked with actinomycin D but not with puromycin or cycloheximide. This is the first report demonstrating regulation of NGF synthesis by a steroid hormone in a clonal cell line of glial origin. We propose this system as a model system for the study of the regulation of NGF synthesis and the isolation and analysis of putative precursors to the NGF molecule.

    Original languageEnglish (US)
    Pages (from-to)1535-1543
    Number of pages9
    JournalLife Sciences
    Volume21
    Issue number10
    DOIs
    StatePublished - Nov 15 1977

    Fingerprint

    Nerve Growth Factor
    Cell culture
    Cell Culture Techniques
    Steroids
    Estradiol
    Steroid hormones
    Cells
    Hormones
    Cell Line
    Puromycin
    Peptide Hormones
    Neurology
    Dactinomycin
    Cycloheximide
    Glioma
    Neuroglia
    Neurons
    Rats
    Central Nervous System
    Molecules

    ASJC Scopus subject areas

    • Pharmacology

    Cite this

    Perez-Polo, J. R., Hall, K., Livingston, K., & Westlund, K. (1977). Steroid induction of nerve growth factor synthesis in cell culture. Life Sciences, 21(10), 1535-1543. https://doi.org/10.1016/0024-3205(77)90211-9

    Steroid induction of nerve growth factor synthesis in cell culture. / Perez-Polo, J. R.; Hall, K.; Livingston, K.; Westlund, K.

    In: Life Sciences, Vol. 21, No. 10, 15.11.1977, p. 1535-1543.

    Research output: Contribution to journalArticle

    Perez-Polo, JR, Hall, K, Livingston, K & Westlund, K 1977, 'Steroid induction of nerve growth factor synthesis in cell culture', Life Sciences, vol. 21, no. 10, pp. 1535-1543. https://doi.org/10.1016/0024-3205(77)90211-9
    Perez-Polo JR, Hall K, Livingston K, Westlund K. Steroid induction of nerve growth factor synthesis in cell culture. Life Sciences. 1977 Nov 15;21(10):1535-1543. https://doi.org/10.1016/0024-3205(77)90211-9
    Perez-Polo, J. R. ; Hall, K. ; Livingston, K. ; Westlund, K. / Steroid induction of nerve growth factor synthesis in cell culture. In: Life Sciences. 1977 ; Vol. 21, No. 10. pp. 1535-1543.
    @article{ef7de6216c5f4f788d1ec2d03164e3ec,
    title = "Steroid induction of nerve growth factor synthesis in cell culture",
    abstract = "Nerve growth factor (NGF) is a peptide hormone which is necessary for the development of sympathetic neurons. Exposing a rat central nervous system glioma cell line (C-6) to the steroid hormone 17β-estradiol increases the amount of NGF secreted by these cells into the surrounding medium. This induction is highly specific to 17β-estradiol in that similar steroids do not increase NGF levels. Both NGF activity and protein levels increase upon estradiol stimulation and there is a parallel increase in NGF de novo synthesis. The estradiol effect can be blocked with actinomycin D but not with puromycin or cycloheximide. This is the first report demonstrating regulation of NGF synthesis by a steroid hormone in a clonal cell line of glial origin. We propose this system as a model system for the study of the regulation of NGF synthesis and the isolation and analysis of putative precursors to the NGF molecule.",
    author = "Perez-Polo, {J. R.} and K. Hall and K. Livingston and K. Westlund",
    year = "1977",
    month = "11",
    day = "15",
    doi = "10.1016/0024-3205(77)90211-9",
    language = "English (US)",
    volume = "21",
    pages = "1535--1543",
    journal = "Life Sciences",
    issn = "0024-3205",
    publisher = "Elsevier Inc.",
    number = "10",

    }

    TY - JOUR

    T1 - Steroid induction of nerve growth factor synthesis in cell culture

    AU - Perez-Polo, J. R.

    AU - Hall, K.

    AU - Livingston, K.

    AU - Westlund, K.

    PY - 1977/11/15

    Y1 - 1977/11/15

    N2 - Nerve growth factor (NGF) is a peptide hormone which is necessary for the development of sympathetic neurons. Exposing a rat central nervous system glioma cell line (C-6) to the steroid hormone 17β-estradiol increases the amount of NGF secreted by these cells into the surrounding medium. This induction is highly specific to 17β-estradiol in that similar steroids do not increase NGF levels. Both NGF activity and protein levels increase upon estradiol stimulation and there is a parallel increase in NGF de novo synthesis. The estradiol effect can be blocked with actinomycin D but not with puromycin or cycloheximide. This is the first report demonstrating regulation of NGF synthesis by a steroid hormone in a clonal cell line of glial origin. We propose this system as a model system for the study of the regulation of NGF synthesis and the isolation and analysis of putative precursors to the NGF molecule.

    AB - Nerve growth factor (NGF) is a peptide hormone which is necessary for the development of sympathetic neurons. Exposing a rat central nervous system glioma cell line (C-6) to the steroid hormone 17β-estradiol increases the amount of NGF secreted by these cells into the surrounding medium. This induction is highly specific to 17β-estradiol in that similar steroids do not increase NGF levels. Both NGF activity and protein levels increase upon estradiol stimulation and there is a parallel increase in NGF de novo synthesis. The estradiol effect can be blocked with actinomycin D but not with puromycin or cycloheximide. This is the first report demonstrating regulation of NGF synthesis by a steroid hormone in a clonal cell line of glial origin. We propose this system as a model system for the study of the regulation of NGF synthesis and the isolation and analysis of putative precursors to the NGF molecule.

    UR - http://www.scopus.com/inward/record.url?scp=0017564977&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0017564977&partnerID=8YFLogxK

    U2 - 10.1016/0024-3205(77)90211-9

    DO - 10.1016/0024-3205(77)90211-9

    M3 - Article

    VL - 21

    SP - 1535

    EP - 1543

    JO - Life Sciences

    JF - Life Sciences

    SN - 0024-3205

    IS - 10

    ER -