Steroid induction of nerve growth factor synthesis in cell culture

J. R. Perez-Polo, K. Hall, K. Livingston, K. Westlund

    Research output: Contribution to journalArticle

    72 Scopus citations

    Abstract

    Nerve growth factor (NGF) is a peptide hormone which is necessary for the development of sympathetic neurons. Exposing a rat central nervous system glioma cell line (C-6) to the steroid hormone 17β-estradiol increases the amount of NGF secreted by these cells into the surrounding medium. This induction is highly specific to 17β-estradiol in that similar steroids do not increase NGF levels. Both NGF activity and protein levels increase upon estradiol stimulation and there is a parallel increase in NGF de novo synthesis. The estradiol effect can be blocked with actinomycin D but not with puromycin or cycloheximide. This is the first report demonstrating regulation of NGF synthesis by a steroid hormone in a clonal cell line of glial origin. We propose this system as a model system for the study of the regulation of NGF synthesis and the isolation and analysis of putative precursors to the NGF molecule.

    Original languageEnglish (US)
    Pages (from-to)1535-1543
    Number of pages9
    JournalLife Sciences
    Volume21
    Issue number10
    DOIs
    StatePublished - Nov 15 1977

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Pharmacology, Toxicology and Pharmaceutics(all)

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    Perez-Polo, J. R., Hall, K., Livingston, K., & Westlund, K. (1977). Steroid induction of nerve growth factor synthesis in cell culture. Life Sciences, 21(10), 1535-1543. https://doi.org/10.1016/0024-3205(77)90211-9