Abstract
Nerve growth factor (NGF) is a peptide hormone which is necessary for the development of sympathetic neurons. Exposing a rat central nervous system glioma cell line (C-6) to the steroid hormone 17β-estradiol increases the amount of NGF secreted by these cells into the surrounding medium. This induction is highly specific to 17β-estradiol in that similar steroids do not increase NGF levels. Both NGF activity and protein levels increase upon estradiol stimulation and there is a parallel increase in NGF de novo synthesis. The estradiol effect can be blocked with actinomycin D but not with puromycin or cycloheximide. This is the first report demonstrating regulation of NGF synthesis by a steroid hormone in a clonal cell line of glial origin. We propose this system as a model system for the study of the regulation of NGF synthesis and the isolation and analysis of putative precursors to the NGF molecule.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1535-1543 |
| Number of pages | 9 |
| Journal | Life Sciences |
| Volume | 21 |
| Issue number | 10 |
| DOIs | |
| State | Published - Nov 15 1977 |
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ASJC Scopus subject areas
- Pharmacology
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Steroid induction of nerve growth factor synthesis in cell culture. / Perez-Polo, J. R.; Hall, K.; Livingston, K.; Westlund, K.
In: Life Sciences, Vol. 21, No. 10, 15.11.1977, p. 1535-1543.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Steroid induction of nerve growth factor synthesis in cell culture
AU - Perez-Polo, J. R.
AU - Hall, K.
AU - Livingston, K.
AU - Westlund, K.
PY - 1977/11/15
Y1 - 1977/11/15
N2 - Nerve growth factor (NGF) is a peptide hormone which is necessary for the development of sympathetic neurons. Exposing a rat central nervous system glioma cell line (C-6) to the steroid hormone 17β-estradiol increases the amount of NGF secreted by these cells into the surrounding medium. This induction is highly specific to 17β-estradiol in that similar steroids do not increase NGF levels. Both NGF activity and protein levels increase upon estradiol stimulation and there is a parallel increase in NGF de novo synthesis. The estradiol effect can be blocked with actinomycin D but not with puromycin or cycloheximide. This is the first report demonstrating regulation of NGF synthesis by a steroid hormone in a clonal cell line of glial origin. We propose this system as a model system for the study of the regulation of NGF synthesis and the isolation and analysis of putative precursors to the NGF molecule.
AB - Nerve growth factor (NGF) is a peptide hormone which is necessary for the development of sympathetic neurons. Exposing a rat central nervous system glioma cell line (C-6) to the steroid hormone 17β-estradiol increases the amount of NGF secreted by these cells into the surrounding medium. This induction is highly specific to 17β-estradiol in that similar steroids do not increase NGF levels. Both NGF activity and protein levels increase upon estradiol stimulation and there is a parallel increase in NGF de novo synthesis. The estradiol effect can be blocked with actinomycin D but not with puromycin or cycloheximide. This is the first report demonstrating regulation of NGF synthesis by a steroid hormone in a clonal cell line of glial origin. We propose this system as a model system for the study of the regulation of NGF synthesis and the isolation and analysis of putative precursors to the NGF molecule.
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U2 - 10.1016/0024-3205(77)90211-9
DO - 10.1016/0024-3205(77)90211-9
M3 - Article
VL - 21
SP - 1535
EP - 1543
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 10
ER -