Stimulation of human dermal fibroblasts with interleukin 2

A. Abdullah, R. L. McCauley, David Herndon

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The role of soluble inflammatory mediators in the wound healing process is under investigation. Interleukin 2 (IL2), an immune modulator produced by T lymphocytes, was tested in vitro for its effects on human diploid fibroblasts. Human diploid fibroblasts (1.0 x 104) were incubated for 24 hours in Alpha minimal essential media (Gibco Laboratories, Gaithersburg, Md.) at 37° C in 5% CO2 in air with 500 U/ml of IL2 (Cetus Corp., Emeryville, Calif.). Cells were then pulsed with 3 μCi/ml of tritiated thymidine overnight. The mean increase in the incorporation of tritiated thymidine of the cells treated with IL2 over control cells was 38% (p value < 0.05). Interleukin 2 has a significant effect on the metabolism of human dermal fibroblasts and may accelerate the wound healing process.

Original languageEnglish (US)
Pages (from-to)23-25
Number of pages3
JournalJournal of Burn Care and Rehabilitation
Volume12
Issue number1
StatePublished - 1991

Fingerprint

Interleukin-2
Fibroblasts
Skin
Diploidy
Wound Healing
Thymidine
Air
T-Lymphocytes

ASJC Scopus subject areas

  • Emergency Medicine
  • Rehabilitation
  • Surgery
  • Nursing(all)
  • Health Professions(all)

Cite this

Abdullah, A., McCauley, R. L., & Herndon, D. (1991). Stimulation of human dermal fibroblasts with interleukin 2. Journal of Burn Care and Rehabilitation, 12(1), 23-25.

Stimulation of human dermal fibroblasts with interleukin 2. / Abdullah, A.; McCauley, R. L.; Herndon, David.

In: Journal of Burn Care and Rehabilitation, Vol. 12, No. 1, 1991, p. 23-25.

Research output: Contribution to journalArticle

Abdullah, A, McCauley, RL & Herndon, D 1991, 'Stimulation of human dermal fibroblasts with interleukin 2', Journal of Burn Care and Rehabilitation, vol. 12, no. 1, pp. 23-25.
Abdullah, A. ; McCauley, R. L. ; Herndon, David. / Stimulation of human dermal fibroblasts with interleukin 2. In: Journal of Burn Care and Rehabilitation. 1991 ; Vol. 12, No. 1. pp. 23-25.
@article{e47d1201bed948d0ae8fb33338009ce9,
title = "Stimulation of human dermal fibroblasts with interleukin 2",
abstract = "The role of soluble inflammatory mediators in the wound healing process is under investigation. Interleukin 2 (IL2), an immune modulator produced by T lymphocytes, was tested in vitro for its effects on human diploid fibroblasts. Human diploid fibroblasts (1.0 x 104) were incubated for 24 hours in Alpha minimal essential media (Gibco Laboratories, Gaithersburg, Md.) at 37° C in 5{\%} CO2 in air with 500 U/ml of IL2 (Cetus Corp., Emeryville, Calif.). Cells were then pulsed with 3 μCi/ml of tritiated thymidine overnight. The mean increase in the incorporation of tritiated thymidine of the cells treated with IL2 over control cells was 38{\%} (p value < 0.05). Interleukin 2 has a significant effect on the metabolism of human dermal fibroblasts and may accelerate the wound healing process.",
author = "A. Abdullah and McCauley, {R. L.} and David Herndon",
year = "1991",
language = "English (US)",
volume = "12",
pages = "23--25",
journal = "Journal of Burn Care and Research",
issn = "1559-047X",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Stimulation of human dermal fibroblasts with interleukin 2

AU - Abdullah, A.

AU - McCauley, R. L.

AU - Herndon, David

PY - 1991

Y1 - 1991

N2 - The role of soluble inflammatory mediators in the wound healing process is under investigation. Interleukin 2 (IL2), an immune modulator produced by T lymphocytes, was tested in vitro for its effects on human diploid fibroblasts. Human diploid fibroblasts (1.0 x 104) were incubated for 24 hours in Alpha minimal essential media (Gibco Laboratories, Gaithersburg, Md.) at 37° C in 5% CO2 in air with 500 U/ml of IL2 (Cetus Corp., Emeryville, Calif.). Cells were then pulsed with 3 μCi/ml of tritiated thymidine overnight. The mean increase in the incorporation of tritiated thymidine of the cells treated with IL2 over control cells was 38% (p value < 0.05). Interleukin 2 has a significant effect on the metabolism of human dermal fibroblasts and may accelerate the wound healing process.

AB - The role of soluble inflammatory mediators in the wound healing process is under investigation. Interleukin 2 (IL2), an immune modulator produced by T lymphocytes, was tested in vitro for its effects on human diploid fibroblasts. Human diploid fibroblasts (1.0 x 104) were incubated for 24 hours in Alpha minimal essential media (Gibco Laboratories, Gaithersburg, Md.) at 37° C in 5% CO2 in air with 500 U/ml of IL2 (Cetus Corp., Emeryville, Calif.). Cells were then pulsed with 3 μCi/ml of tritiated thymidine overnight. The mean increase in the incorporation of tritiated thymidine of the cells treated with IL2 over control cells was 38% (p value < 0.05). Interleukin 2 has a significant effect on the metabolism of human dermal fibroblasts and may accelerate the wound healing process.

UR - http://www.scopus.com/inward/record.url?scp=0026088916&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026088916&partnerID=8YFLogxK

M3 - Article

VL - 12

SP - 23

EP - 25

JO - Journal of Burn Care and Research

JF - Journal of Burn Care and Research

SN - 1559-047X

IS - 1

ER -