Stimulation of muscle protein synthesis by long-term insulin infusion in severely burned patients

Y. Sakurai, A. Aarsland, David Herndon, D. L. Chinkes, E. Pierre, T. T. Nguyen, B. W. Patterson, R. R. Wolfe, M. F. Brennan, B. A. Pruitt, N. N. Abumrad, J. E. Fischer, P. Bessey, W. Cioffi

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Abstract

Objective: To determine if long-term (7 days) infusion of insulin can ameliorate altered protein kinetics in skeletal muscle of severely burned patients and to investigate the hypothesis that changes in protein kinetics during insulin infusion are associated with an increased rate of transmembrane amino acid transport from plasma into the intracellular free amino acid pool. Summary Background Data: In critically ill patients, vigorous nutritional support alone may often fail to entirely curtail muscle catabolism; insulin stimulates muscle protein synthesis in normal volunteers. Methods: Nine patients with severe burns were studied once during enteral feeding alone (control period), and once after 7 days of high-dose insulin. The order of treatment with insulin was randomized. Data were derived from a model based on a primed continuous infusion of L-[15N]phenylalanine, sampling of blood from the femoral artery and vein, and biopsies of the vastus lateralis muscle. Results: Net leg muscle protein balance was significantly (p < 0.05) negative during the control period. Exogenous insulin eliminated this negative balance by stimulating protein synthesis approximately 350% (p < 0.01). This was made possible in part by a sixfold increase in the inward transport of amino acids from blood (p < 0.01). There was also a significant increase in leg muscle protein breakdown. The new rates of synthesis, breakdown, and inward transport during insulin were in balance, such that there was no difference in the intracellular phenylalanine concentration from the control period. The fractional synthetic rate of protein in the wound was also stimulated by insulin by approximately 50%, but the response was variable and did not reach significance. Conclusions: Exogenous insulin may be useful in promoting muscle protein synthesis in severely catabolic patients.

Original languageEnglish (US)
Pages (from-to)283-297
Number of pages15
JournalAnnals of Surgery
Volume222
Issue number3
StatePublished - 1995

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Muscle Proteins
Insulin
Phenylalanine
Amino Acids
Leg
Proteins
Muscles
Femoral Vein
Nutritional Support
Quadriceps Muscle
Enteral Nutrition
Femoral Artery
Burns
Critical Illness
Healthy Volunteers
Skeletal Muscle
Biopsy
Wounds and Injuries

ASJC Scopus subject areas

  • Surgery

Cite this

Sakurai, Y., Aarsland, A., Herndon, D., Chinkes, D. L., Pierre, E., Nguyen, T. T., ... Cioffi, W. (1995). Stimulation of muscle protein synthesis by long-term insulin infusion in severely burned patients. Annals of Surgery, 222(3), 283-297.

Stimulation of muscle protein synthesis by long-term insulin infusion in severely burned patients. / Sakurai, Y.; Aarsland, A.; Herndon, David; Chinkes, D. L.; Pierre, E.; Nguyen, T. T.; Patterson, B. W.; Wolfe, R. R.; Brennan, M. F.; Pruitt, B. A.; Abumrad, N. N.; Fischer, J. E.; Bessey, P.; Cioffi, W.

In: Annals of Surgery, Vol. 222, No. 3, 1995, p. 283-297.

Research output: Contribution to journalArticle

Sakurai, Y, Aarsland, A, Herndon, D, Chinkes, DL, Pierre, E, Nguyen, TT, Patterson, BW, Wolfe, RR, Brennan, MF, Pruitt, BA, Abumrad, NN, Fischer, JE, Bessey, P & Cioffi, W 1995, 'Stimulation of muscle protein synthesis by long-term insulin infusion in severely burned patients', Annals of Surgery, vol. 222, no. 3, pp. 283-297.
Sakurai Y, Aarsland A, Herndon D, Chinkes DL, Pierre E, Nguyen TT et al. Stimulation of muscle protein synthesis by long-term insulin infusion in severely burned patients. Annals of Surgery. 1995;222(3):283-297.
Sakurai, Y. ; Aarsland, A. ; Herndon, David ; Chinkes, D. L. ; Pierre, E. ; Nguyen, T. T. ; Patterson, B. W. ; Wolfe, R. R. ; Brennan, M. F. ; Pruitt, B. A. ; Abumrad, N. N. ; Fischer, J. E. ; Bessey, P. ; Cioffi, W. / Stimulation of muscle protein synthesis by long-term insulin infusion in severely burned patients. In: Annals of Surgery. 1995 ; Vol. 222, No. 3. pp. 283-297.
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abstract = "Objective: To determine if long-term (7 days) infusion of insulin can ameliorate altered protein kinetics in skeletal muscle of severely burned patients and to investigate the hypothesis that changes in protein kinetics during insulin infusion are associated with an increased rate of transmembrane amino acid transport from plasma into the intracellular free amino acid pool. Summary Background Data: In critically ill patients, vigorous nutritional support alone may often fail to entirely curtail muscle catabolism; insulin stimulates muscle protein synthesis in normal volunteers. Methods: Nine patients with severe burns were studied once during enteral feeding alone (control period), and once after 7 days of high-dose insulin. The order of treatment with insulin was randomized. Data were derived from a model based on a primed continuous infusion of L-[15N]phenylalanine, sampling of blood from the femoral artery and vein, and biopsies of the vastus lateralis muscle. Results: Net leg muscle protein balance was significantly (p < 0.05) negative during the control period. Exogenous insulin eliminated this negative balance by stimulating protein synthesis approximately 350{\%} (p < 0.01). This was made possible in part by a sixfold increase in the inward transport of amino acids from blood (p < 0.01). There was also a significant increase in leg muscle protein breakdown. The new rates of synthesis, breakdown, and inward transport during insulin were in balance, such that there was no difference in the intracellular phenylalanine concentration from the control period. The fractional synthetic rate of protein in the wound was also stimulated by insulin by approximately 50{\%}, but the response was variable and did not reach significance. Conclusions: Exogenous insulin may be useful in promoting muscle protein synthesis in severely catabolic patients.",
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T1 - Stimulation of muscle protein synthesis by long-term insulin infusion in severely burned patients

AU - Sakurai, Y.

AU - Aarsland, A.

AU - Herndon, David

AU - Chinkes, D. L.

AU - Pierre, E.

AU - Nguyen, T. T.

AU - Patterson, B. W.

AU - Wolfe, R. R.

AU - Brennan, M. F.

AU - Pruitt, B. A.

AU - Abumrad, N. N.

AU - Fischer, J. E.

AU - Bessey, P.

AU - Cioffi, W.

PY - 1995

Y1 - 1995

N2 - Objective: To determine if long-term (7 days) infusion of insulin can ameliorate altered protein kinetics in skeletal muscle of severely burned patients and to investigate the hypothesis that changes in protein kinetics during insulin infusion are associated with an increased rate of transmembrane amino acid transport from plasma into the intracellular free amino acid pool. Summary Background Data: In critically ill patients, vigorous nutritional support alone may often fail to entirely curtail muscle catabolism; insulin stimulates muscle protein synthesis in normal volunteers. Methods: Nine patients with severe burns were studied once during enteral feeding alone (control period), and once after 7 days of high-dose insulin. The order of treatment with insulin was randomized. Data were derived from a model based on a primed continuous infusion of L-[15N]phenylalanine, sampling of blood from the femoral artery and vein, and biopsies of the vastus lateralis muscle. Results: Net leg muscle protein balance was significantly (p < 0.05) negative during the control period. Exogenous insulin eliminated this negative balance by stimulating protein synthesis approximately 350% (p < 0.01). This was made possible in part by a sixfold increase in the inward transport of amino acids from blood (p < 0.01). There was also a significant increase in leg muscle protein breakdown. The new rates of synthesis, breakdown, and inward transport during insulin were in balance, such that there was no difference in the intracellular phenylalanine concentration from the control period. The fractional synthetic rate of protein in the wound was also stimulated by insulin by approximately 50%, but the response was variable and did not reach significance. Conclusions: Exogenous insulin may be useful in promoting muscle protein synthesis in severely catabolic patients.

AB - Objective: To determine if long-term (7 days) infusion of insulin can ameliorate altered protein kinetics in skeletal muscle of severely burned patients and to investigate the hypothesis that changes in protein kinetics during insulin infusion are associated with an increased rate of transmembrane amino acid transport from plasma into the intracellular free amino acid pool. Summary Background Data: In critically ill patients, vigorous nutritional support alone may often fail to entirely curtail muscle catabolism; insulin stimulates muscle protein synthesis in normal volunteers. Methods: Nine patients with severe burns were studied once during enteral feeding alone (control period), and once after 7 days of high-dose insulin. The order of treatment with insulin was randomized. Data were derived from a model based on a primed continuous infusion of L-[15N]phenylalanine, sampling of blood from the femoral artery and vein, and biopsies of the vastus lateralis muscle. Results: Net leg muscle protein balance was significantly (p < 0.05) negative during the control period. Exogenous insulin eliminated this negative balance by stimulating protein synthesis approximately 350% (p < 0.01). This was made possible in part by a sixfold increase in the inward transport of amino acids from blood (p < 0.01). There was also a significant increase in leg muscle protein breakdown. The new rates of synthesis, breakdown, and inward transport during insulin were in balance, such that there was no difference in the intracellular phenylalanine concentration from the control period. The fractional synthetic rate of protein in the wound was also stimulated by insulin by approximately 50%, but the response was variable and did not reach significance. Conclusions: Exogenous insulin may be useful in promoting muscle protein synthesis in severely catabolic patients.

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