Stimulation of neutrophil leukocyte chemotaxis by a cloned cytolytic enterotoxin of Aeromonas hydrophila

Gui Fang Jin, Ashok Chopra, Clifford W. Houston

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A cytolytic enterotoxin produced by Aeromonas hydrophila, isolate SSU, has been cloned in our laboratory. This enterotoxin lysed rabbit red blood cells, destroyed Chinese hamster ovary cells, caused fluid secretion in rat ligated ileal loops, inhibited the phagocytic function of mouse phagocytes, and was lethal to mice when injected intravenously. In this study, the effect of this cytolytic enterotoxin on the chemotaxis of human leukocytes (cell line RPMI 1788) was examined,. This toxin, at concentrations from 92.5 to 370 ng/ml, significantly stimulated the chemotactic activity of human leukocytes in a dose-dependent fashion. The stimulation of leukocyte chemotaxis elicited by cytolytic enterotoxin was abolished when the toxin was neutralized, heated, or exposed to low pH values. This stimulatory effect also was inhibited by various concentrations of pertussis toxin. These results demonstrated that cytolytic enterotoxin may stimulate increased chemotaxis of human leukocytes, and suggest that human leukocytes may possess cytolytic enterotoxin receptors which may be coupled to pertussis toxin-sensitive G-protein.

Original languageEnglish (US)
Pages (from-to)285-289
Number of pages5
JournalFEMS Microbiology Letters
Volume98
Issue number1-3
StatePublished - Nov 1 1992

Fingerprint

Leukocyte Chemotaxis
Aeromonas hydrophila
Enterotoxins
Neutrophils
Pertussis Toxin
Leukocytes
Fluids and Secretions
Phagocytes
Cricetulus
GTP-Binding Proteins
Human Activities
Ovary
Erythrocytes
Rabbits
Cell Line

Keywords

  • Aeromonas hydrophila
  • Chemotaxis
  • Cytolytic enterotoxin
  • Leukocyte
  • Pertussis toxin

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Stimulation of neutrophil leukocyte chemotaxis by a cloned cytolytic enterotoxin of Aeromonas hydrophila. / Jin, Gui Fang; Chopra, Ashok; Houston, Clifford W.

In: FEMS Microbiology Letters, Vol. 98, No. 1-3, 01.11.1992, p. 285-289.

Research output: Contribution to journalArticle

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