Stimulation of prohormone convertase-1 mRNA expression by second messenger signaling systems

Vidyavathi Udupi, Courtney M. Townsend, George H. Greeley

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


The purpose of this study was to investigate the effects of activation of various second messenger signaling systems on gene expression (ie. mRNA levels) of a peptide hormone processing enzyme called prohormone convertase-1 (PC-1, also called PC-3) in a human pancreatic carcinoid cell line (BON) that expresses several endocrine peptides (chromogranin A, pancreastatin, neurotensin). Pharmacologic activation of adenylate cyclase-cAMP, protein kinase-C and Ca2+ mobilization pathways increased PC-1 mRNA levels and neurotensin secretion. Elevations in PC-1 mRNA levels were dose and time-related. Secretagogue-induced cellular depletion of neurotensin was followed by a replenishment of cellular neurotensin stores and an upregulation of PC-1 mRNA levels. Together, these data indicate that PC-1 mRNA expression is increased with peptide secretion and coordinated with maintenance of cellular stores of peptides.

Original languageEnglish (US)
Pages (from-to)463-465
Number of pages3
JournalBiochemical and Biophysical Research Communications
Issue number2
StatePublished - May 19 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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