Stimulation of prohormone convertase-1 mRNA expression by second messenger signaling systems

Vidyavathi Udupi, Courtney M. Townsend, George H. Greeley

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The purpose of this study was to investigate the effects of activation of various second messenger signaling systems on gene expression (ie. mRNA levels) of a peptide hormone processing enzyme called prohormone convertase-1 (PC-1, also called PC-3) in a human pancreatic carcinoid cell line (BON) that expresses several endocrine peptides (chromogranin A, pancreastatin, neurotensin). Pharmacologic activation of adenylate cyclase-cAMP, protein kinase-C and Ca2+ mobilization pathways increased PC-1 mRNA levels and neurotensin secretion. Elevations in PC-1 mRNA levels were dose and time-related. Secretagogue-induced cellular depletion of neurotensin was followed by a replenishment of cellular neurotensin stores and an upregulation of PC-1 mRNA levels. Together, these data indicate that PC-1 mRNA expression is increased with peptide secretion and coordinated with maintenance of cellular stores of peptides.

Original languageEnglish (US)
Pages (from-to)463-465
Number of pages3
JournalBiochemical and Biophysical Research Communications
Volume246
Issue number2
DOIs
StatePublished - May 19 1998

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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