TY - JOUR
T1 - Stimulation of prohormone convertase-1 mRNA expression by second messenger signaling systems
AU - Udupi, Vidyavathi
AU - Townsend, Courtney M.
AU - Greeley, George H.
N1 - Funding Information:
This work was supported by a grant from the National Institutes of Health (P01 DK35608).
PY - 1998/5/19
Y1 - 1998/5/19
N2 - The purpose of this study was to investigate the effects of activation of various second messenger signaling systems on gene expression (ie. mRNA levels) of a peptide hormone processing enzyme called prohormone convertase-1 (PC-1, also called PC-3) in a human pancreatic carcinoid cell line (BON) that expresses several endocrine peptides (chromogranin A, pancreastatin, neurotensin). Pharmacologic activation of adenylate cyclase-cAMP, protein kinase-C and Ca2+ mobilization pathways increased PC-1 mRNA levels and neurotensin secretion. Elevations in PC-1 mRNA levels were dose and time-related. Secretagogue-induced cellular depletion of neurotensin was followed by a replenishment of cellular neurotensin stores and an upregulation of PC-1 mRNA levels. Together, these data indicate that PC-1 mRNA expression is increased with peptide secretion and coordinated with maintenance of cellular stores of peptides.
AB - The purpose of this study was to investigate the effects of activation of various second messenger signaling systems on gene expression (ie. mRNA levels) of a peptide hormone processing enzyme called prohormone convertase-1 (PC-1, also called PC-3) in a human pancreatic carcinoid cell line (BON) that expresses several endocrine peptides (chromogranin A, pancreastatin, neurotensin). Pharmacologic activation of adenylate cyclase-cAMP, protein kinase-C and Ca2+ mobilization pathways increased PC-1 mRNA levels and neurotensin secretion. Elevations in PC-1 mRNA levels were dose and time-related. Secretagogue-induced cellular depletion of neurotensin was followed by a replenishment of cellular neurotensin stores and an upregulation of PC-1 mRNA levels. Together, these data indicate that PC-1 mRNA expression is increased with peptide secretion and coordinated with maintenance of cellular stores of peptides.
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U2 - 10.1006/bbrc.1998.8643
DO - 10.1006/bbrc.1998.8643
M3 - Article
C2 - 9610384
AN - SCOPUS:0032546494
SN - 0006-291X
VL - 246
SP - 463
EP - 465
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -