Stimulation of prohormone convertase-1 mRNA expression by second messenger signaling systems

Vidyavathi Udupi; Courtney M. Townsend; George H. Greeley

doi:10.1006/bbrc.1998.8643

Stimulation of prohormone convertase-1 mRNA expression by second messenger signaling systems

Vidyavathi Udupi
, Courtney M. Townsend
, George H. Greeley

Surgery

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

The purpose of this study was to investigate the effects of activation of various second messenger signaling systems on gene expression (ie. mRNA levels) of a peptide hormone processing enzyme called prohormone convertase-1 (PC-1, also called PC-3) in a human pancreatic carcinoid cell line (BON) that expresses several endocrine peptides (chromogranin A, pancreastatin, neurotensin). Pharmacologic activation of adenylate cyclase-cAMP, protein kinase-C and Ca²⁺ mobilization pathways increased PC-1 mRNA levels and neurotensin secretion. Elevations in PC-1 mRNA levels were dose and time-related. Secretagogue-induced cellular depletion of neurotensin was followed by a replenishment of cellular neurotensin stores and an upregulation of PC-1 mRNA levels. Together, these data indicate that PC-1 mRNA expression is increased with peptide secretion and coordinated with maintenance of cellular stores of peptides.

Original language	English (US)
Pages (from-to)	463-465
Number of pages	3
Journal	Biochemical and Biophysical Research Communications
Volume	246
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1998.8643
State	Published - May 19 1998

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Stimulation of prohormone convertase-1 mRNA expression by second messenger signaling systems",

abstract = "The purpose of this study was to investigate the effects of activation of various second messenger signaling systems on gene expression (ie. mRNA levels) of a peptide hormone processing enzyme called prohormone convertase-1 (PC-1, also called PC-3) in a human pancreatic carcinoid cell line (BON) that expresses several endocrine peptides (chromogranin A, pancreastatin, neurotensin). Pharmacologic activation of adenylate cyclase-cAMP, protein kinase-C and Ca2+ mobilization pathways increased PC-1 mRNA levels and neurotensin secretion. Elevations in PC-1 mRNA levels were dose and time-related. Secretagogue-induced cellular depletion of neurotensin was followed by a replenishment of cellular neurotensin stores and an upregulation of PC-1 mRNA levels. Together, these data indicate that PC-1 mRNA expression is increased with peptide secretion and coordinated with maintenance of cellular stores of peptides.",

author = "Vidyavathi Udupi and Townsend, \{Courtney M.\} and Greeley, \{George H.\}",

note = "Funding Information: This work was supported by a grant from the National Institutes of Health (P01 DK35608).",

year = "1998",

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doi = "10.1006/bbrc.1998.8643",

language = "English (US)",

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T1 - Stimulation of prohormone convertase-1 mRNA expression by second messenger signaling systems

AU - Udupi, Vidyavathi

AU - Townsend, Courtney M.

AU - Greeley, George H.

N1 - Funding Information: This work was supported by a grant from the National Institutes of Health (P01 DK35608).

PY - 1998/5/19

Y1 - 1998/5/19

N2 - The purpose of this study was to investigate the effects of activation of various second messenger signaling systems on gene expression (ie. mRNA levels) of a peptide hormone processing enzyme called prohormone convertase-1 (PC-1, also called PC-3) in a human pancreatic carcinoid cell line (BON) that expresses several endocrine peptides (chromogranin A, pancreastatin, neurotensin). Pharmacologic activation of adenylate cyclase-cAMP, protein kinase-C and Ca2+ mobilization pathways increased PC-1 mRNA levels and neurotensin secretion. Elevations in PC-1 mRNA levels were dose and time-related. Secretagogue-induced cellular depletion of neurotensin was followed by a replenishment of cellular neurotensin stores and an upregulation of PC-1 mRNA levels. Together, these data indicate that PC-1 mRNA expression is increased with peptide secretion and coordinated with maintenance of cellular stores of peptides.

AB - The purpose of this study was to investigate the effects of activation of various second messenger signaling systems on gene expression (ie. mRNA levels) of a peptide hormone processing enzyme called prohormone convertase-1 (PC-1, also called PC-3) in a human pancreatic carcinoid cell line (BON) that expresses several endocrine peptides (chromogranin A, pancreastatin, neurotensin). Pharmacologic activation of adenylate cyclase-cAMP, protein kinase-C and Ca2+ mobilization pathways increased PC-1 mRNA levels and neurotensin secretion. Elevations in PC-1 mRNA levels were dose and time-related. Secretagogue-induced cellular depletion of neurotensin was followed by a replenishment of cellular neurotensin stores and an upregulation of PC-1 mRNA levels. Together, these data indicate that PC-1 mRNA expression is increased with peptide secretion and coordinated with maintenance of cellular stores of peptides.

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DO - 10.1006/bbrc.1998.8643

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AN - SCOPUS:0032546494

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VL - 246

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EP - 465

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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ER -

Stimulation of prohormone convertase-1 mRNA expression by second messenger signaling systems

Abstract

ASJC Scopus subject areas

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