Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor

Evelyn Tiffany-Castiglioni, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    Resistance of human neuroblastoma cell line SK-N-SH-SY5Y (SY5Y) to the neurotoxin 6-hydroxydopamine (6-OHDA) was established by exposure of the cells to nerve growth factor (NGF). SY5Y cells display several properties of immature sympathetic nerve cells, including morphological responses to nerve growth factor and susceptibility to cytolysis by 6-OHDA. High resistance to 6-OHDA was achieved by culturing SY5Y cells with NGF. Protection persisted when NGF was absent for 24 h or when cells were treated with colcemid. In contrast, dibutyryl cyclic AMP did not stimulate resistance to 6-OHDA. NGF-treated cells were also resistant to H2O2, a toxic product of 6-OHDA autoxidation.

    Original languageEnglish (US)
    Pages (from-to)157-161
    Number of pages5
    JournalNeuroscience Letters
    Volume26
    Issue number2
    DOIs
    StatePublished - Oct 23 1981

    Fingerprint

    Oxidopamine
    Nerve Growth Factor
    Neuroblastoma
    Cell Line
    Demecolcine
    Bucladesine
    Poisons
    Neurotoxins
    Neurons

    Keywords

    • 6-hydroxydopamine
    • cytotoxicity
    • hydrogen peroxide
    • nerve growth factor
    • neuroblastoma

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor. / Tiffany-Castiglioni, Evelyn; Perez-Polo, J. Regino.

    In: Neuroscience Letters, Vol. 26, No. 2, 23.10.1981, p. 157-161.

    Research output: Contribution to journalArticle

    Tiffany-Castiglioni, Evelyn ; Perez-Polo, J. Regino. / Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor. In: Neuroscience Letters. 1981 ; Vol. 26, No. 2. pp. 157-161.
    @article{1925f825d02d45abb573c1792783b473,
    title = "Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor",
    abstract = "Resistance of human neuroblastoma cell line SK-N-SH-SY5Y (SY5Y) to the neurotoxin 6-hydroxydopamine (6-OHDA) was established by exposure of the cells to nerve growth factor (NGF). SY5Y cells display several properties of immature sympathetic nerve cells, including morphological responses to nerve growth factor and susceptibility to cytolysis by 6-OHDA. High resistance to 6-OHDA was achieved by culturing SY5Y cells with NGF. Protection persisted when NGF was absent for 24 h or when cells were treated with colcemid. In contrast, dibutyryl cyclic AMP did not stimulate resistance to 6-OHDA. NGF-treated cells were also resistant to H2O2, a toxic product of 6-OHDA autoxidation.",
    keywords = "6-hydroxydopamine, cytotoxicity, hydrogen peroxide, nerve growth factor, neuroblastoma",
    author = "Evelyn Tiffany-Castiglioni and Perez-Polo, {J. Regino}",
    year = "1981",
    month = "10",
    day = "23",
    doi = "10.1016/0304-3940(81)90342-6",
    language = "English (US)",
    volume = "26",
    pages = "157--161",
    journal = "Neuroscience Letters",
    issn = "0304-3940",
    publisher = "Elsevier Ireland Ltd",
    number = "2",

    }

    TY - JOUR

    T1 - Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor

    AU - Tiffany-Castiglioni, Evelyn

    AU - Perez-Polo, J. Regino

    PY - 1981/10/23

    Y1 - 1981/10/23

    N2 - Resistance of human neuroblastoma cell line SK-N-SH-SY5Y (SY5Y) to the neurotoxin 6-hydroxydopamine (6-OHDA) was established by exposure of the cells to nerve growth factor (NGF). SY5Y cells display several properties of immature sympathetic nerve cells, including morphological responses to nerve growth factor and susceptibility to cytolysis by 6-OHDA. High resistance to 6-OHDA was achieved by culturing SY5Y cells with NGF. Protection persisted when NGF was absent for 24 h or when cells were treated with colcemid. In contrast, dibutyryl cyclic AMP did not stimulate resistance to 6-OHDA. NGF-treated cells were also resistant to H2O2, a toxic product of 6-OHDA autoxidation.

    AB - Resistance of human neuroblastoma cell line SK-N-SH-SY5Y (SY5Y) to the neurotoxin 6-hydroxydopamine (6-OHDA) was established by exposure of the cells to nerve growth factor (NGF). SY5Y cells display several properties of immature sympathetic nerve cells, including morphological responses to nerve growth factor and susceptibility to cytolysis by 6-OHDA. High resistance to 6-OHDA was achieved by culturing SY5Y cells with NGF. Protection persisted when NGF was absent for 24 h or when cells were treated with colcemid. In contrast, dibutyryl cyclic AMP did not stimulate resistance to 6-OHDA. NGF-treated cells were also resistant to H2O2, a toxic product of 6-OHDA autoxidation.

    KW - 6-hydroxydopamine

    KW - cytotoxicity

    KW - hydrogen peroxide

    KW - nerve growth factor

    KW - neuroblastoma

    UR - http://www.scopus.com/inward/record.url?scp=0019805906&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0019805906&partnerID=8YFLogxK

    U2 - 10.1016/0304-3940(81)90342-6

    DO - 10.1016/0304-3940(81)90342-6

    M3 - Article

    VL - 26

    SP - 157

    EP - 161

    JO - Neuroscience Letters

    JF - Neuroscience Letters

    SN - 0304-3940

    IS - 2

    ER -