Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor

Evelyn Tiffany-Castiglioni; J. Regino Perez-Polo

doi:10.1016/0304-3940(81)90342-6

Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor

Evelyn Tiffany-Castiglioni, J. Regino Perez-Polo

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Resistance of human neuroblastoma cell line SK-N-SH-SY5Y (SY5Y) to the neurotoxin 6-hydroxydopamine (6-OHDA) was established by exposure of the cells to nerve growth factor (NGF). SY5Y cells display several properties of immature sympathetic nerve cells, including morphological responses to nerve growth factor and susceptibility to cytolysis by 6-OHDA. High resistance to 6-OHDA was achieved by culturing SY5Y cells with NGF. Protection persisted when NGF was absent for 24 h or when cells were treated with colcemid. In contrast, dibutyryl cyclic AMP did not stimulate resistance to 6-OHDA. NGF-treated cells were also resistant to H₂O₂, a toxic product of 6-OHDA autoxidation.

Original language	English (US)
Pages (from-to)	157-161
Number of pages	5
Journal	Neuroscience Letters
Volume	26
Issue number	2
DOIs	https://doi.org/10.1016/0304-3940(81)90342-6
State	Published - Oct 23 1981
Externally published	Yes

Keywords

6-hydroxydopamine
cytotoxicity
hydrogen peroxide
nerve growth factor
neuroblastoma

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(81)90342-6

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title = "Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor",

abstract = "Resistance of human neuroblastoma cell line SK-N-SH-SY5Y (SY5Y) to the neurotoxin 6-hydroxydopamine (6-OHDA) was established by exposure of the cells to nerve growth factor (NGF). SY5Y cells display several properties of immature sympathetic nerve cells, including morphological responses to nerve growth factor and susceptibility to cytolysis by 6-OHDA. High resistance to 6-OHDA was achieved by culturing SY5Y cells with NGF. Protection persisted when NGF was absent for 24 h or when cells were treated with colcemid. In contrast, dibutyryl cyclic AMP did not stimulate resistance to 6-OHDA. NGF-treated cells were also resistant to H2O2, a toxic product of 6-OHDA autoxidation.",

keywords = "6-hydroxydopamine, cytotoxicity, hydrogen peroxide, nerve growth factor, neuroblastoma",

author = "Evelyn Tiffany-Castiglioni and Perez-Polo, {J. Regino}",

note = "Funding Information: We thank I. Schulze for helpful discussion of the manuscript; C. Beck and K. Werrbach-Perez for excellent technical assistance and B. Dzambo for manuscript preparation. This work was supported by an NIH National Research Service Award GM07204 to E.T.C., and NINCDS Grants NS14034 and NS15324, Robert A. Welch Grant H698, and an RCDA (NS00213) to J.R.P.-P.",

year = "1981",

month = oct,

day = "23",

doi = "10.1016/0304-3940(81)90342-6",

language = "English (US)",

volume = "26",

pages = "157--161",

journal = "Neuroscience Letters",

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T1 - Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor

AU - Tiffany-Castiglioni, Evelyn

AU - Perez-Polo, J. Regino

N1 - Funding Information: We thank I. Schulze for helpful discussion of the manuscript; C. Beck and K. Werrbach-Perez for excellent technical assistance and B. Dzambo for manuscript preparation. This work was supported by an NIH National Research Service Award GM07204 to E.T.C., and NINCDS Grants NS14034 and NS15324, Robert A. Welch Grant H698, and an RCDA (NS00213) to J.R.P.-P.

PY - 1981/10/23

Y1 - 1981/10/23

N2 - Resistance of human neuroblastoma cell line SK-N-SH-SY5Y (SY5Y) to the neurotoxin 6-hydroxydopamine (6-OHDA) was established by exposure of the cells to nerve growth factor (NGF). SY5Y cells display several properties of immature sympathetic nerve cells, including morphological responses to nerve growth factor and susceptibility to cytolysis by 6-OHDA. High resistance to 6-OHDA was achieved by culturing SY5Y cells with NGF. Protection persisted when NGF was absent for 24 h or when cells were treated with colcemid. In contrast, dibutyryl cyclic AMP did not stimulate resistance to 6-OHDA. NGF-treated cells were also resistant to H2O2, a toxic product of 6-OHDA autoxidation.

AB - Resistance of human neuroblastoma cell line SK-N-SH-SY5Y (SY5Y) to the neurotoxin 6-hydroxydopamine (6-OHDA) was established by exposure of the cells to nerve growth factor (NGF). SY5Y cells display several properties of immature sympathetic nerve cells, including morphological responses to nerve growth factor and susceptibility to cytolysis by 6-OHDA. High resistance to 6-OHDA was achieved by culturing SY5Y cells with NGF. Protection persisted when NGF was absent for 24 h or when cells were treated with colcemid. In contrast, dibutyryl cyclic AMP did not stimulate resistance to 6-OHDA. NGF-treated cells were also resistant to H2O2, a toxic product of 6-OHDA autoxidation.

KW - 6-hydroxydopamine

KW - cytotoxicity

KW - hydrogen peroxide

KW - nerve growth factor

KW - neuroblastoma

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JF - Neuroscience Letters

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ER -

Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor

Abstract

Keywords

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