Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by nerve growth factor

Evelyn Tiffany-Castiglioni, J. Regino Perez-Polo

    Research output: Contribution to journalArticle

    30 Scopus citations

    Abstract

    Resistance of human neuroblastoma cell line SK-N-SH-SY5Y (SY5Y) to the neurotoxin 6-hydroxydopamine (6-OHDA) was established by exposure of the cells to nerve growth factor (NGF). SY5Y cells display several properties of immature sympathetic nerve cells, including morphological responses to nerve growth factor and susceptibility to cytolysis by 6-OHDA. High resistance to 6-OHDA was achieved by culturing SY5Y cells with NGF. Protection persisted when NGF was absent for 24 h or when cells were treated with colcemid. In contrast, dibutyryl cyclic AMP did not stimulate resistance to 6-OHDA. NGF-treated cells were also resistant to H2O2, a toxic product of 6-OHDA autoxidation.

    Original languageEnglish (US)
    Pages (from-to)157-161
    Number of pages5
    JournalNeuroscience Letters
    Volume26
    Issue number2
    DOIs
    StatePublished - Oct 23 1981

    Keywords

    • 6-hydroxydopamine
    • cytotoxicity
    • hydrogen peroxide
    • nerve growth factor
    • neuroblastoma

    ASJC Scopus subject areas

    • Neuroscience(all)

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