Stimulation of specific Aeromonas antibody secretion in rat intestine by γ-aminobutyric acid

Gui Fang Jin, Yan Shi Guo, Clifford W. Houston

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study was undertaken to assess a possible role for γ-aminobutyric acid (GABA) in the regulation of intestinal secretion of IgA and IgG antibodies. Rats were immunized with culture supernatant of Aeromonas hydrophila isolate SSU. This culture supernatant contains a number of toxins that may be considered virulence factors. After 24 days of immunization, rats were anesthetized and a 10-cm intestinal segment was intubated and ligated at both ends in situ. The intestinal loop was perfused with phosphate-buffered saline (PBS). The effluents were collected for measurement of IgA and IgG by the ELISA. When compared with the effect of intravenous administration of normal saline in the control group, intravenous injection of GABA (30 mg/kg) resulted in a significant increase of IgA and IgG secretion in the experimental group. These stimulatory effects of GABA on secretion of IgA and IgG were abolished by bicuculline, a GABA-receptor antagonist, and by atropine, indicating that the GABA-stimulated secretion of IgA and IgG was mediated via the GABA receptors and cholinergic muscarinic receptors. These results suggest that GABA may participate in the nervous regulation of intestinal secretion of IgA and IgG antibodies in the rat.

Original languageEnglish (US)
Pages (from-to)617-622
Number of pages6
JournalDigestive Diseases and Sciences
Volume34
Issue number4
DOIs
StatePublished - Apr 1989

Fingerprint

Aminobutyrates
Aeromonas
Immunoglobulin A
Intestines
gamma-Aminobutyric Acid
Immunoglobulin G
Antibodies
Intestinal Secretions
GABA Antagonists
Aeromonas hydrophila
GABA Receptors
Bicuculline
Cholinergic Receptors
Muscarinic Receptors
Virulence Factors
Atropine
Intravenous Injections
Intravenous Administration
Immunization
Enzyme-Linked Immunosorbent Assay

Keywords

  • Aeromonas hydrophila
  • GABA
  • IgA
  • IgG

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Stimulation of specific Aeromonas antibody secretion in rat intestine by γ-aminobutyric acid. / Jin, Gui Fang; Guo, Yan Shi; Houston, Clifford W.

In: Digestive Diseases and Sciences, Vol. 34, No. 4, 04.1989, p. 617-622.

Research output: Contribution to journalArticle

Jin, Gui Fang ; Guo, Yan Shi ; Houston, Clifford W. / Stimulation of specific Aeromonas antibody secretion in rat intestine by γ-aminobutyric acid. In: Digestive Diseases and Sciences. 1989 ; Vol. 34, No. 4. pp. 617-622.
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