Stress signaler p38 mitogen-activated kinase activation: a cause for concern?

Enkhtuya Radnaa, Lauren Richardson, Brett Goldman, Jared K. Burks, Tuvshintugs Baljinnyam, Natasha Vora, Hui Juan Zhang, Elizabeth A. Bonney, Arum Han, Ramkumar Menon

Research output: Contribution to journalArticlepeer-review

Abstract

Oxidative stress (OS) induced activation of p38 mitogen-activated kinase (MAPK) and cell fate from p38 signaling was tested using the human fetal membrane's amnion epithelial cells (AEC). We created p38 KO AEC using the CRISPR/Cas9 approach and tested cell fate in response to OS on an AEC-free fetal membrane extracellular matrix (ECM). Screening using image CyTOF indicated OS causing epithelial-mesenchymal transition (EMT). Further testing revealed p38 deficiency prevented AEC senescence, EMT, cell migration, and inflammation. To functionally validate in vitro findings, fetal membrane-specific conditional KO (cKO) mice were developed by injecting Cre-recombinase encoded exosomes intra-amniotically into p38αloxP/loxP mice. Amnion membranes from p38 cKO mice had reduced senescence, EMT, and increased anti-inflammatory IL-10 compared with WT animals. Our study suggested that overwhelming activation of p38 in response to OS inducing risk exposures can have an adverse impact on cells, cause cell invasion, inflammation, and ECM degradation detrimental to tissue homeostasis.

Original languageEnglish (US)
Pages (from-to)1591-1614
Number of pages24
JournalClinical Science
Volume136
Issue number22
DOIs
StatePublished - Nov 30 2022
Externally publishedYes

Keywords

  • EMT
  • Senescence
  • pregnancy

ASJC Scopus subject areas

  • Medicine(all)

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