Stretching single talin rod molecules activates vinculin binding

Armando Del Rio, Raul Perez-Jimenez, Ruchuan Liu, Pere Roca-Cusachs, Julio M. Fernandez, Michael Sheetz

Research output: Contribution to journalArticle

818 Citations (Scopus)

Abstract

The molecular mechanism by which a mechanical stimulus is translated into a chemical response in biological systems is still unclear. We show that mechanical stretching of single cytoplasmic proteins can activate binding of other molecules. We used magnetic tweezers, total internal reflection fluorescence, and atomic force microscopy to investigate the effect of force on the interaction between talin, a protein that links liganded membrane integrins to the cytoskeleton, and vinculin, a focal adhesion protein that is activated by talin binding, leading to reorganization of the cytoskeleton. Application of physiologically relevant forces caused stretching of single talin rods that exposed cryptic binding sites for vinculin. Thus in the talin-vinculin system, molecular mechanotransduction can occur by protein binding after exposure of buried binding sites in the talin-vinculin system. Such protein stretching may be a more general mechanism for force transduction.

Original languageEnglish (US)
Pages (from-to)638-641
Number of pages4
JournalScience
Volume323
Issue number5914
DOIs
StatePublished - Jan 30 2009
Externally publishedYes

Fingerprint

Talin
Vinculin
Cytoskeleton
Binding Sites
Proteins
Focal Adhesions
Atomic Force Microscopy
Protein Binding
Integrins
Fluorescence
Membranes

ASJC Scopus subject areas

  • General

Cite this

Del Rio, A., Perez-Jimenez, R., Liu, R., Roca-Cusachs, P., Fernandez, J. M., & Sheetz, M. (2009). Stretching single talin rod molecules activates vinculin binding. Science, 323(5914), 638-641. https://doi.org/10.1126/science.1162912

Stretching single talin rod molecules activates vinculin binding. / Del Rio, Armando; Perez-Jimenez, Raul; Liu, Ruchuan; Roca-Cusachs, Pere; Fernandez, Julio M.; Sheetz, Michael.

In: Science, Vol. 323, No. 5914, 30.01.2009, p. 638-641.

Research output: Contribution to journalArticle

Del Rio, A, Perez-Jimenez, R, Liu, R, Roca-Cusachs, P, Fernandez, JM & Sheetz, M 2009, 'Stretching single talin rod molecules activates vinculin binding', Science, vol. 323, no. 5914, pp. 638-641. https://doi.org/10.1126/science.1162912
Del Rio A, Perez-Jimenez R, Liu R, Roca-Cusachs P, Fernandez JM, Sheetz M. Stretching single talin rod molecules activates vinculin binding. Science. 2009 Jan 30;323(5914):638-641. https://doi.org/10.1126/science.1162912
Del Rio, Armando ; Perez-Jimenez, Raul ; Liu, Ruchuan ; Roca-Cusachs, Pere ; Fernandez, Julio M. ; Sheetz, Michael. / Stretching single talin rod molecules activates vinculin binding. In: Science. 2009 ; Vol. 323, No. 5914. pp. 638-641.
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