Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment

Iryna Pinchuk, Katherine T. Morris, Robert A. Nofchissey, Rachel B. Earley, Jeng Yih Wu, Thomas Y. Ma, Ellen J. Beswick

Research output: Contribution to journalArticle

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Abstract

Gastric cancer is associated with chronic inflammation and Helicobacter pylori infection. Th17 cells are CD4+ T cells associated with infections and inflammation; but their role and mechanism of induction during carcinogenesis is not understood. Gastric myofibroblasts/fibroblasts (GMF) are abundant class II MHC expressing cells that act as novel antigen presenting cells. Here we have demonstrated the accumulation of Th17 in H. pylori-infected human tissues and in the gastric tumor microenvironment. GMF isolated from human gastric cancer and H. pylori infected tissues co-cultured with CD4+ T cells induced substantially higher levels of Th17 than GMF from normal tissues in an IL-6, TGF-β, and IL-21 dependent manner. Th17 required interaction with class II MHC on GMF for activation and proliferation. These studies suggest that Th17 are induced during both H. pylori infection and gastric cancer in the inflammatory milieu of gastric stroma and may be an important link between inflammation and carcinogenesis.

Original languageEnglish (US)
Article numbere53798
JournalPLoS One
Volume8
Issue number1
DOIs
StatePublished - Jan 30 2013

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Tumor Microenvironment
stomach neoplasms
Helicobacter pylori
stromal cells
Helicobacter Infections
Fibroblasts
Stromal Cells
Tumors
Stomach
stomach
Myofibroblasts
fibroblasts
T-cells
Tissue
infection
inflammation
Stomach Neoplasms
carcinogenesis
Inflammation
T-lymphocytes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Pinchuk, I., Morris, K. T., Nofchissey, R. A., Earley, R. B., Wu, J. Y., Ma, T. Y., & Beswick, E. J. (2013). Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment. PLoS One, 8(1), [e53798]. https://doi.org/10.1371/journal.pone.0053798

Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment. / Pinchuk, Iryna; Morris, Katherine T.; Nofchissey, Robert A.; Earley, Rachel B.; Wu, Jeng Yih; Ma, Thomas Y.; Beswick, Ellen J.

In: PLoS One, Vol. 8, No. 1, e53798, 30.01.2013.

Research output: Contribution to journalArticle

Pinchuk, I, Morris, KT, Nofchissey, RA, Earley, RB, Wu, JY, Ma, TY & Beswick, EJ 2013, 'Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment', PLoS One, vol. 8, no. 1, e53798. https://doi.org/10.1371/journal.pone.0053798
Pinchuk, Iryna ; Morris, Katherine T. ; Nofchissey, Robert A. ; Earley, Rachel B. ; Wu, Jeng Yih ; Ma, Thomas Y. ; Beswick, Ellen J. / Stromal Cells Induce Th17 during Helicobacter pylori Infection and in the Gastric Tumor Microenvironment. In: PLoS One. 2013 ; Vol. 8, No. 1.
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