Stromelysins in placental membranes and amniotic fluid with premature rupture of membranes

Stephen J. Fortunato, Ramkumar Menon, Salvatore J. Lombardi

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Objective: To determine the expression and site of production of stromelysins in fetal membranes and to measure stromelysin 1 levels in amniotic fluid and amniochorion culture media. Methods: Amniochorionic membranes were cultured from organ explant. Membranes were stimulated with lipopolysaccharide for 24 hours after a 48-hour preincubation period. Membranes were also collected from women after vaginal deliveries. RNA samples from those tissues were subjected to reverse transcriptase-polymerase chain reaction using primers specific for stromelysin 1, stromelysin 2, stromelysin 3, and matrilysin. In situ hybridization and immunohistochemistry were used to localize stromelysin mRNA and peptide. Levels of stromelysin 1 in culture media and amniotic fluid collected from women with preterm premature rupture of membranes (PROM) and at term with intact membranes were compared using enzyme-linked immunosorbant assay. Results: Amniochorion in culture and from laboring and nonlaboring women expressed all three stromelysins. In situ hybridization showed stromelysin mRNA in amnion, chorion, and extracellular matrix. Immunohistochemical analysis localized stromelysin 1 protein to those same regions. Amniotic fluid levels of stromelysin 1 were higher in preterm PROM amniotic fluids (median 3.2 ng/mL) compared with term deliveries with intact membranes (median 1.3 ng/mL) (P = .02). Lipopolysaccharide stimulation in culture increased the release of stromelysin 1 from fetal membranes compared with control (median 70.35 versus 15.8 ng/mL, respectively, P = .05). Conclusion: Human fetal membranes are a source of stromelysins 1, 2, and 3. Increased stromelysin 1 during preterm PROM and in vitro after lipopolysaccharide stimulation suggests a possible effect of that matrix metalloproteinase in PROM. Copyright (C) 1999 The American College of Obstetricians and Gynecologists.

Original languageEnglish (US)
Pages (from-to)435-440
Number of pages6
JournalObstetrics and Gynecology
Volume94
Issue number3
DOIs
StatePublished - Sep 1999
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 3
Amniotic Fluid
Rupture
Membranes
Extraembryonic Membranes
Matrix Metalloproteinase 10
Matrix Metalloproteinase 11
Lipopolysaccharides
In Situ Hybridization
Culture Media
Matrix Metalloproteinase 7
Chorion
Messenger RNA
Amnion
Reverse Transcriptase Polymerase Chain Reaction
Matrix Metalloproteinases
Extracellular Matrix

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Stromelysins in placental membranes and amniotic fluid with premature rupture of membranes. / Fortunato, Stephen J.; Menon, Ramkumar; Lombardi, Salvatore J.

In: Obstetrics and Gynecology, Vol. 94, No. 3, 09.1999, p. 435-440.

Research output: Contribution to journalArticle

Fortunato, Stephen J. ; Menon, Ramkumar ; Lombardi, Salvatore J. / Stromelysins in placental membranes and amniotic fluid with premature rupture of membranes. In: Obstetrics and Gynecology. 1999 ; Vol. 94, No. 3. pp. 435-440.
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