Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

Bei wei Zhu, Dong mei Li, Da yong Zhou, Song Han, Jing feng Yang, Tao Li, Wen xiu Ye, George H. Greeley

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

Original languageEnglish (US)
Pages (from-to)1273-1278
Number of pages6
JournalFood Chemistry
Volume125
Issue number4
DOIs
StatePublished - Apr 15 2011

Fingerprint

Haliotis discus hannai
cholecystokinin
Viscera
Cholecystokinin
abalone
animal organs
Galactose
Structural analysis
galactose
Polysaccharides
Rhamnose
Fucose
fucose
polysaccharides
rhamnose
Xylose
Gels
Chromatography
xylose
Gel Chromatography

Keywords

  • Abalone (Haliotis Discus Hannai Ino)
  • Cholecystokinin
  • Polysaccharide
  • Viscera

ASJC Scopus subject areas

  • Food Science
  • Analytical Chemistry

Cite this

Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera. / Zhu, Bei wei; Li, Dong mei; Zhou, Da yong; Han, Song; Yang, Jing feng; Li, Tao; Ye, Wen xiu; Greeley, George H.

In: Food Chemistry, Vol. 125, No. 4, 15.04.2011, p. 1273-1278.

Research output: Contribution to journalArticle

Zhu, Bei wei ; Li, Dong mei ; Zhou, Da yong ; Han, Song ; Yang, Jing feng ; Li, Tao ; Ye, Wen xiu ; Greeley, George H. / Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera. In: Food Chemistry. 2011 ; Vol. 125, No. 4. pp. 1273-1278.
@article{8a83c16c975e429684ea2862c823196c,
title = "Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera",
abstract = "A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.",
keywords = "Abalone (Haliotis Discus Hannai Ino), Cholecystokinin, Polysaccharide, Viscera",
author = "Zhu, {Bei wei} and Li, {Dong mei} and Zhou, {Da yong} and Song Han and Yang, {Jing feng} and Tao Li and Ye, {Wen xiu} and Greeley, {George H.}",
year = "2011",
month = "4",
day = "15",
doi = "10.1016/j.foodchem.2010.10.065",
language = "English (US)",
volume = "125",
pages = "1273--1278",
journal = "Food Chemistry",
issn = "0308-8146",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

AU - Zhu, Bei wei

AU - Li, Dong mei

AU - Zhou, Da yong

AU - Han, Song

AU - Yang, Jing feng

AU - Li, Tao

AU - Ye, Wen xiu

AU - Greeley, George H.

PY - 2011/4/15

Y1 - 2011/4/15

N2 - A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

AB - A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

KW - Abalone (Haliotis Discus Hannai Ino)

KW - Cholecystokinin

KW - Polysaccharide

KW - Viscera

UR - http://www.scopus.com/inward/record.url?scp=78649324080&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649324080&partnerID=8YFLogxK

U2 - 10.1016/j.foodchem.2010.10.065

DO - 10.1016/j.foodchem.2010.10.065

M3 - Article

VL - 125

SP - 1273

EP - 1278

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

IS - 4

ER -