Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

Bei wei Zhu, Dong mei Li, Da yong Zhou, Song Han, Jing feng Yang, Tao Li, Wen xiu Ye, George H. Greeley

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

Original languageEnglish (US)
Pages (from-to)1273-1278
Number of pages6
JournalFood Chemistry
Volume125
Issue number4
DOIs
StatePublished - Apr 15 2011
Externally publishedYes

Keywords

  • Abalone (Haliotis Discus Hannai Ino)
  • Cholecystokinin
  • Polysaccharide
  • Viscera

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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