Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

Bei wei Zhu, Dong mei Li, Da yong Zhou, Song Han, Jing feng Yang, Tao Li, Wen xiu Ye, George H. Greeley

    Research output: Contribution to journalArticlepeer-review

    28 Scopus citations

    Abstract

    A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

    Original languageEnglish (US)
    Pages (from-to)1273-1278
    Number of pages6
    JournalFood Chemistry
    Volume125
    Issue number4
    DOIs
    StatePublished - Apr 15 2011

    Keywords

    • Abalone (Haliotis Discus Hannai Ino)
    • Cholecystokinin
    • Polysaccharide
    • Viscera

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Food Science

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