Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

Bei wei Zhu; Dong mei Li; Da yong Zhou; Song Han; Jing feng Yang; Tao Li; Wen xiu Ye; George H. Greeley

doi:10.1016/j.foodchem.2010.10.065

Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

Bei wei Zhu
, Dong mei Li
, Da yong Zhou
, Song Han
, Jing feng Yang
, Tao Li
, Wen xiu Ye
, George H. Greeley

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PW_XL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

Original language	English (US)
Pages (from-to)	1273-1278
Number of pages	6
Journal	Food Chemistry
Volume	125
Issue number	4
DOIs	https://doi.org/10.1016/j.foodchem.2010.10.065
State	Published - Apr 15 2011
Externally published	Yes

Keywords

Abalone (Haliotis Discus Hannai Ino)
Cholecystokinin
Polysaccharide
Viscera

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2010.10.065

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@article{8a83c16c975e429684ea2862c823196c,

title = "Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera",

abstract = "A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.",

keywords = "Abalone (Haliotis Discus Hannai Ino), Cholecystokinin, Polysaccharide, Viscera",

author = "Zhu, \{Bei wei\} and Li, \{Dong mei\} and Zhou, \{Da yong\} and Song Han and Yang, \{Jing feng\} and Tao Li and Ye, \{Wen xiu\} and Greeley, \{George H.\}",

note = "Funding Information: This work was financially supported by “ The National High Technology Research and Development Program of China (863 Program) (No. 2007AA091804 )”, “The National Great Project of Scientific and Technical Supporting Programs Funded by Ministry of Science \& Technology of China During the 11th Five-year Plan (Nos. 2008BAD94B07 ; 2008BAD94B03 )”, and “The General Research Project of Scientific and Technical Supporting Programs Funded by Liaoning Education Department (No. 2009A082 )”.",

year = "2011",

month = apr,

day = "15",

doi = "10.1016/j.foodchem.2010.10.065",

language = "English (US)",

volume = "125",

pages = "1273--1278",

journal = "Food Chemistry",

issn = "0308-8146",

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number = "4",

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TY - JOUR

T1 - Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

AU - Zhu, Bei wei

AU - Li, Dong mei

AU - Zhou, Da yong

AU - Han, Song

AU - Yang, Jing feng

AU - Li, Tao

AU - Ye, Wen xiu

AU - Greeley, George H.

N1 - Funding Information: This work was financially supported by “ The National High Technology Research and Development Program of China (863 Program) (No. 2007AA091804 )”, “The National Great Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China During the 11th Five-year Plan (Nos. 2008BAD94B07 ; 2008BAD94B03 )”, and “The General Research Project of Scientific and Technical Supporting Programs Funded by Liaoning Education Department (No. 2009A082 )”.

PY - 2011/4/15

Y1 - 2011/4/15

N2 - A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

AB - A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

KW - Abalone (Haliotis Discus Hannai Ino)

KW - Cholecystokinin

KW - Polysaccharide

KW - Viscera

UR - https://www.scopus.com/pages/publications/78649324080

UR - https://www.scopus.com/pages/publications/78649324080#tab=citedBy

U2 - 10.1016/j.foodchem.2010.10.065

DO - 10.1016/j.foodchem.2010.10.065

M3 - Article

AN - SCOPUS:78649324080

SN - 0308-8146

VL - 125

SP - 1273

EP - 1278

JO - Food Chemistry

JF - Food Chemistry

IS - 4

ER -

Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

Abstract

Keywords

ASJC Scopus subject areas

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