Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

Bei wei Zhu; Dong mei Li; Da yong Zhou; Song Han; Jing feng Yang; Tao Li; Wen xiu Ye; George H. Greeley

doi:10.1016/j.foodchem.2010.10.065

Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

Bei wei Zhu, Dong mei Li, Da yong Zhou, Song Han, Jing feng Yang, Tao Li, Wen xiu Ye, George H. Greeley

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PW_XL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

Original language	English (US)
Pages (from-to)	1273-1278
Number of pages	6
Journal	Food Chemistry
Volume	125
Issue number	4
DOIs	https://doi.org/10.1016/j.foodchem.2010.10.065
State	Published - Apr 15 2011
Externally published	Yes

Keywords

Abalone (Haliotis Discus Hannai Ino)
Cholecystokinin
Polysaccharide
Viscera

ASJC Scopus subject areas

Analytical Chemistry
Food Science

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10.1016/j.foodchem.2010.10.065

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@article{8a83c16c975e429684ea2862c823196c,

title = "Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera",

abstract = "A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.",

keywords = "Abalone (Haliotis Discus Hannai Ino), Cholecystokinin, Polysaccharide, Viscera",

author = "Zhu, \{Bei wei\} and Li, \{Dong mei\} and Zhou, \{Da yong\} and Song Han and Yang, \{Jing feng\} and Tao Li and Ye, \{Wen xiu\} and Greeley, \{George H.\}",

note = "Funding Information: This work was financially supported by “ The National High Technology Research and Development Program of China (863 Program) (No. 2007AA091804 )”, “The National Great Project of Scientific and Technical Supporting Programs Funded by Ministry of Science \& Technology of China During the 11th Five-year Plan (Nos. 2008BAD94B07 ; 2008BAD94B03 )”, and “The General Research Project of Scientific and Technical Supporting Programs Funded by Liaoning Education Department (No. 2009A082 )”.",

year = "2011",

month = apr,

day = "15",

doi = "10.1016/j.foodchem.2010.10.065",

language = "English (US)",

volume = "125",

pages = "1273--1278",

journal = "Food Chemistry",

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T1 - Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

AU - Zhu, Bei wei

AU - Li, Dong mei

AU - Zhou, Da yong

AU - Han, Song

AU - Yang, Jing feng

AU - Li, Tao

AU - Ye, Wen xiu

AU - Greeley, George H.

N1 - Funding Information: This work was financially supported by “ The National High Technology Research and Development Program of China (863 Program) (No. 2007AA091804 )”, “The National Great Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China During the 11th Five-year Plan (Nos. 2008BAD94B07 ; 2008BAD94B03 )”, and “The General Research Project of Scientific and Technical Supporting Programs Funded by Liaoning Education Department (No. 2009A082 )”.

PY - 2011/4/15

Y1 - 2011/4/15

N2 - A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

AB - A fraction of water-soluble sulphated polysaccharide conjugate, termed AHP-2, was obtained from abalone (Haliotis Discus Hannai Ino) viscera by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-gel G4000PWXL column and gel filtration chromatography on Sepharose CL-6B indicated AHP-2 is homogenous with an average molecular weight (MW) of about 11.0kDa. The structure of AHP-2 was revealed by chemical methods, Fourier transform infrared (FTIR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated AHP-2 is a heteroglycan consisting of glucose, fucose, xylose, rhamnose and galactose with molar ratio of 1.0:2.0:3.9:6.7:7.4. The backbone of AHP-2 consists of 1,3-linked rhamnose and 1,3,6-linked galactose, with glucose, fucose, xylose and galactose of different linkage types distributing in branched chains. Meanwhile, AHP-2 was found to increase cholecystokinin (CCK) release in CCK-secreting STC-1 cells.

KW - Abalone (Haliotis Discus Hannai Ino)

KW - Cholecystokinin

KW - Polysaccharide

KW - Viscera

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DO - 10.1016/j.foodchem.2010.10.065

M3 - Article

AN - SCOPUS:78649324080

SN - 0308-8146

VL - 125

SP - 1273

EP - 1278

JO - Food Chemistry

JF - Food Chemistry

IS - 4

ER -

Structural analysis and CCK-releasing activity of a sulphated polysaccharide from abalone (Haliotis Discus Hannai Ino) viscera

Abstract

Keywords

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